A5084242240- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Immune cells in cancer
- Periodontal Regeneration and Treatments
- Cancer Immunotherapy and Biomarkers
- Liver physiology and pathology
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Genetics, Aging, and Longevity in Model Organisms
- Bone Tissue Engineering Materials
- Air Quality and Health Impacts
- Immunotherapy and Immune Responses
- Mast cells and histamine
- Pain Mechanisms and Treatments
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
- Chemokine receptors and signaling
- Acupuncture Treatment Research Studies
- Cholesterol and Lipid Metabolism
- Cytokine Signaling Pathways and Interactions
- Cleft Lip and Palate Research
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- interferon and immune responses
- Advanced Glycation End Products research
Vita-Salute San Raffaele University
2020-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2020-2023
University of Milan
2012-2017
Istituto Ortopedico Galeazzi
2016
Abstract Painful neuropathy is one of the complications diabetes mellitus that adversely affects patients’quality life. Pharmacological treatments are not fully satisfactory, and novel approaches needed. In a preclinical mouse model effect both human mesenchymal stromal cells from adipose tissue (hASC) their conditioned medium (hASC-CM) was evaluated. Diabetes induced by streptozotocin. After neuropathic hypersensitivity established, mice were intravenously injected with either 1 × 10 6 hASC...
Gingival tissue is composed of cell types that contribute to the body's defense against many agents in oral environment, wound healing and regeneration. Thanks their easy scarcely invasive withdrawal procedure, interdental papilla provide a good source mesenchymal stromal cells (GinPa-MSCs). We isolated GinPa-MSCs verified ability uptake/release anticancer agent Paclitaxel (PTX).In vitro expanded were characterized for CD markers by FACS, tested differentiation analyzed TEM. Their PTX was...
Acute inflammation is a complex biological response of tissues to harmful stimuli, such as pathogens or cell damage, and essential for immune defence proper healing. However, unresolved can lead chronic disorders, including cancer fibrosis. The High Mobility Group Box 1 (HMGB1) protein Damage-Associated Molecular Pattern (DAMP) molecule that orchestrates key events in by switching among mutually exclusive redox states. Fully reduced HMGB1 (fr-HMGB1) supports recruitment tissue regeneration,...
Over the last years, advancements in use of nanoparticles for biomedical applications have clearly showcased their potential preparation improved imaging and drug-delivery systems. However, compared to vast number currently studied such applications, only a few successfully translate into clinical practice. A common "barrier" that prevents from efficiently delivering payload target site after administration is related liver filtering, mainly due nanoparticle uptake by macrophages. This work...
Abstract Introduction Adipose-derived stem cells (ASCs) are progenitor used in bone tissue engineering and regenerative medicine. Despite subcutaneous adipose being more abundant, the buccal fat pad (BFP) is easily accessible for dentists maxillofacial surgeons. For this reason, considering need preclinical study swine as an optimal animal model applications, we compared features of porcine ASCs (pASCs) from both tissue-harvesting sites. Methods were isolated interscapular (ScI) pads six...
Abstract Exogenous IgE acts as an adjuvant in tumor vaccination mice, and therefore a direct role of endogenous immunosurveillance was investigated. By using genetically engineered we found that ablation rendered mice more susceptible to the growth transplantable tumors. Conversely, strengthened response provided with partial or complete resistance growth, depending on type. genetic crosses, showed IgE-mediated protection mostly lost lacking FcεRI. Tumor also after depletion CD8+ T cells,...
Adipose-derived stem/stromal cells (ASCs) are progenitor used in bone tissue engineering and regenerative medicine. Since Bichat's fat pad is easily accessible for dentists maxillo-facial surgeons, we compared the features of ASCs from (BFP-ASCs) with human subcutaneous adipose (SC-ASCs). BFP-ASCs isolated a small amount were characterized their stemness multidifferentiative ability. They showed an important clonogenic ability typical mesenchymal stem cell immunophenotype. Moreover, when...
Food-grade titanium dioxide (E171) contains variable percentages of (TiO
Abstract Lipid and cholesterol metabolism play a crucial role in tumor cell behavior shaping the microenvironment. In particular, enzymatic non-enzymatic metabolism, derived metabolites control dendritic (DC) functions, ultimately impacting antigen presentation within outside mass, dampening immunity immunotherapeutic attempts. The mechanisms accounting for such events remain largely to be defined. Here we perturbed (oxy)sterol genetically pharmacologically analyzed lipidome landscape...
In the study, we assess a rapid prototyped scaffold composed of 30/70 hydroxyapatite (HA) and beta-tricalcium-phosphate (β-TCP) loaded with human adipose-derived stem cells (hASCs) to determine cell proliferation, differentiation toward osteogenic lineage, adhesion penetration on/into scaffold.In this in vitro hASCs isolated from fat tissue discarded after plastic surgery were expanded, characterized, then onto scaffold. Cells tested for: viability assay (Alamar Blue at days 3, 7 Live/Dead...
Hepatic metastases are a poor prognostic factor of colorectal carcinoma (CRC) and new strategies to reduce the risk liver CRC colonization highly needed. Herein, we used mouse models hepatic metastatization demonstrate that continuous infusion therapeutic doses interferon-alpha (IFNα) controls invasion by acting on endothelial cells (HECs). Mechanistically, IFNα promoted development vascular antimetastatic niche characterized sinusoidal (LSECs) defenestration extracellular matrix glycocalyx...
Food-grade titanium dioxide (E171) contains variable percentages of TiO2 nanoparticles (NPs) posing awareness about its potential effects on human and animal health. Despite many studies, the actual relationship between physicochemical properties E171 NPs their interaction with biological targets is still far from clear. We evaluated acute toxicity in invertebrate vertebrate animals. In nematode C. elegans, up to 1.0 mg/mL did not affect worm’s viability lifespan but significantly...
Nowadays, the repair of large bone defects is an important goal in orthopaedic and dental fields. Tissue engineering, applied to increase regeneration process, combines suitable scaffolds with either terminally differentiated cells or Mesenchymal Stromal Cells. In vitro studies Adipose-derived Cells (ASCs) may identify new bioactive supports, be tested preclinical model. this study, we evaluated biocompatibility osteoinductive properties two substitutes, RegenOSS™ (RO-1) a generation...
Abstract Hepatic metastases are a poor prognostic factor of colorectal carcinoma (CRC) and new strategies to reduce the risk liver CRC colonization highly needed. Herein, we used mouse models hepatic metastatization demonstrate that continuous infusion therapeutic doses interferon-alpha (IFNα) controls invasion by acting on endothelial cells (HECs) . Mechanistically, IFNα promoted development vascular antimetastatic niche characterized sinusoidal (LSECs) defenestration extracellular matrix...