A5075657141- Immunotherapy and Immune Responses
- Cholesterol and Lipid Metabolism
- T-cell and B-cell Immunology
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Protist diversity and phylogeny
- Biocrusts and Microbial Ecology
- vaccines and immunoinformatics approaches
- Cancer, Lipids, and Metabolism
- Fungal Biology and Applications
- Photoreceptor and optogenetics research
- Reproductive Biology and Fertility
- Sphingolipid Metabolism and Signaling
- Cancer Research and Treatments
- Light effects on plants
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Slime Mold and Myxomycetes Research
- bioluminescence and chemiluminescence research
- Drug Transport and Resistance Mechanisms
- Animal Genetics and Reproduction
- Plant Pathogens and Fungal Diseases
Vita-Salute San Raffaele University
2005-2025
IRCCS Ospedale San Raffaele
2013-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
University of Naples Federico II
2024
The San Raffaele Telethon Institute for Gene Therapy
2009-2022
San Raffaele University of Rome
2007-2018
Gene Therapy Laboratory
2008-2014
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2014
Mylan (Switzerland)
2014
Thirty-nine tumor-bearing patients with metastatic melanoma were treated 3 subcutaneous injections of the MAGE-3.A1 peptide at monthly intervals. No significant toxicity was observed. Of 25 who received complete treatment, 7 displayed tumor regressions. All but one these regressions involved cutaneous metastases. Three and 2 led to a disease-free state, which persisted for more than years after beginning treatment. evidence cytolytic T lymphocyte (CTL) response found in blood 4 analyzed,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural and functional features of the .alpha.3 chain indicate a bridging role for chicken collagen VI in connective tissuesPaolo Bonaldo, Vincenzo Russo, Francesco Bucciotti, Roberto Doliana, Alfonso ColombattiCite this: Biochemistry 1990, 29, 5, 1245–1254Publication Date (Print):February 6, 1990Publication History Published online1 May 2002Published inissue 6 February...
Tumor-infiltrating immune cells can be conditioned by molecules released within the microenvironment to thwart antitumor responses, thereby facilitating tumor growth. Among cells, neutrophils play an important protumorigenic role favoring neoangiogenesis and/or suppressing responses. Tumor-derived oxysterols have recently been shown favor growth inhibiting dendritic cell migration toward lymphoid organs. We report that tumor-derived recruit protumor in a liver X receptor (LXR)–independent,...
Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene N. encoding carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis its structure regulation. The encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed level mRNA increased over 70-fold in photoinduced Transcription run-on studies indicated was at initiation transcription...
F R Lauter, V E Russo, and C Yanofsky Department of Biological Sciences, Stanford University, California 94305.
MAGE genes are expressed by many human tumors of different histological types but not normal cells, except for male germline cells. The Ags encoded and recognized T cells therefore strictly tumor-specific. Clinical trials involving therapeutic vaccination cancer patients with antigenic peptides or proteins in progress. To increase the range eligible therapy peptides, it is important to identify additional epitopes CTL. Candidate known bind a given HLA have been used stimulate lymphocytes...
In an attempt to transduce monocyte-derived dendritic cells (DCs) with a retroviral vector coding for intracytoplasmic tumor antigen (TAA), we were confronted by the evident dissociation between ability of treated DCs induce TAA-specific response, and presence integrated proviral DNA. The TAA, i.e., MAGE-3, was acquired presented immune effectors, thanks property uptake apoptotic bodies released irradiated vector-producing cells. Indeed, observed that upon irradiation underwent cell death,...
Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene N. encoding carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis its structure regulation. The encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed level mRNA increased over 70-fold in photoinduced Transcription run-on studies indicated was at initiation transcription...
Abstract Pentraxins (PTX) and complement belong to the humoral arm of innate immune system have essential functions in defense microbes scavenging cellular debris. The prototypic long PTX, PTX3, first component classical pathway, C1q, are opsonins involved disposal dying cells by phagocytes. Whether interaction between various impacts on their function is not fully understood. We show here that characterized Toll-like receptor (TLR) ligands elicit production C1q PTX3 immature dendritic (DC)....
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities.It is possible to extract more than 50 minipreps at once.
White collar (wc) mutants of Neurospora crassa are thought to be regulatory blocked in the photoinduction carotenogenesis. Eight new wc have been isolated after UV mutagenesis; their morphology and linear growth rate not altered, although blue light-induced carotenogenesis is completely blocked. All mutations fall into two complementation groups corresponding already-known wc-1 wc-2 loci. It shown that impair another light effect, protoperithecia formation, as well low constitutive...
The CXC chemokine CXCL8/IL-8 plays a major role in the activation and recruitment of polymorphonuclear (PMN) cells at inflammatory sites. CXCL8 activates PMNs by binding seven-transmembrane (7-TM) G-protein-coupled receptors receptor 1 (CXCR1) 2 (CXCR2). (R)-Ketoprofen (1) was previously reported to be potent specific noncompetitive inhibitor CXCL8-induced human chemotaxis. We report here molecular modeling studies showing putative interaction site TM region CXCR1. model confirmed alanine...
Neuroblastoma (NB), the most common solid extracranial cancer of childhood, displays a remarkable low expression Major Histocompatibility Complex class I (MHC-I) and Antigen Processing Machinery (APM) molecules, including Endoplasmic Reticulum (ER) Aminopeptidases, poorly presents tumor antigens to Cytotoxic T Lymphocytes (CTL). We have previously shown that this is due transcription factor NF-kB p65. Herein, we show not only p65, but also Interferon Regulatory Factor 1 (IRF1) certain APM...
By stimulating human CD8+ T lymphocytes with autologous dendritic cells infected an adenovirus encoding MAGE-3, we obtained a cytotoxic lymphocyte (CTL) clone that recognized new MAGE-3 antigenic peptide, AELVHFLLL, which is presented by HLA-B40. This peptide also encoded MAGE-12. The CTL MAGE-3–expressing tumor only when they were first treated IFN-γ. Since this treatment known to induce the exchange of three catalytic subunits proteasome form immunoproteasome, result suggested processing...
We have cloned the al-2 gene of Neurospora crassa and analyzed its structure regulation. The encodes a 603-residue polypeptide with segment homologous to prokaryotic other eukaryotic phytoene synthases. RNA measurements showed that level mRNA increased over 30-fold in photoinduced mycelia compared dark-grown mycelia. This observation is consistent fact carotenoid biosynthesis induced by blue light during growth N. increase levels was not observed two mutants, wc-1 wc-2, are defective all...