A5067274552- Pain Mechanisms and Treatments
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- T-cell and B-cell Immunology
- Ophthalmology and Eye Disorders
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Anesthesia and Pain Management
- Cancer, Stress, Anesthesia, and Immune Response
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Atherosclerosis and Cardiovascular Diseases
- Thyroid Disorders and Treatments
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytokine Signaling Pathways and Interactions
- IL-33, ST2, and ILC Pathways
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Menopause: Health Impacts and Treatments
- Trigeminal Neuralgia and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Ocular Surface and Contact Lens
- Migraine and Headache Studies
- Pain Management and Placebo Effect
University of Milan
2017-2025
Istituto Nazionale Genetica Molecolare
2021-2025
Institute of Molecular Genetics
2023
Politecnico di Milano
2016
Three-dimensional (3D) structured organoids are the most advanced in vitro models for studying human health effects, but their application to evaluate biological effects associated with microplastic exposure was neglected until now. Fibers from synthetic clothes and fabrics a major source of airborne microplastics, release dryer machines is poorly understood. We quantified characterized fibers (MPFs) released exhaust filter household tested on airway (1, 10, 50 µg mL-1) by optical...
Abstract Painful neuropathy is one of the complications diabetes mellitus that adversely affects patients’quality life. Pharmacological treatments are not fully satisfactory, and novel approaches needed. In a preclinical mouse model effect both human mesenchymal stromal cells from adipose tissue (hASC) their conditioned medium (hASC-CM) was evaluated. Diabetes induced by streptozotocin. After neuropathic hypersensitivity established, mice were intravenously injected with either 1 × 10 6 hASC...
Introduction: Impaired immune function during the perioperative period may be associated with worse short- and long-term outcomes. Morphine is considered a major contributor to modulation. Patients methods: We performed pilot study investigate postoperative by analyzing peripheral blood mononuclear cells' functionality cytokine production in 16 patients undergoing abdominal surgery. All were treated intravenous (i.v.) patient-controlled analgesia morphine continuous wound infusion...
Chronic neuropathic pain constitutes a serious public health problem, but the disease mechanisms are only partially understood. The involvement of different brain regions like medial prefrontal cortex has already been established, comparison role subregions and layers is still inconclusive. In current study, we performed patch-clamp recordings followed by anatomical reconstruction pyramidal cells from prelimbic infralimbic in (spared nerve injury, SNI) control mice. We found that cortex,...
The urgent need for safer and innovative antitubercular agents remains a priority the scientific community. In pursuit of this goal, we designed evaluated novel 5-phenylfuran-2-carboxylic acid derivatives targeting Mycobacterium tuberculosis (Mtb) salicylate synthase (MbtI), key enzyme, absent in humans, that plays crucial role Mtb virulence. Several potent MbtI inhibitors demonstrating significant activity favorable safety profile were identified. Structure-guided optimization yielded...
Neuropathy is a dose-limiting side effect of many chemotherapeutics, including bortezomib. The mechanisms underlying this condition are not fully elucidated even if contribution neuroinflammation was suggested. Here, we investigated the role chemokine family, prokineticins (PKs), in development bortezomib-induced peripheral neuropathy (BIPN), and used PK receptor antagonist to counteract progression pathology. induced male C57BL/6J mice by using protocol capable induce detectable neuropathic...
IFNγ-producing ex-Th17 cells ['Th1/17'] were shown to play a key pathogenic role in experimental colitis and are abundant the intestine. Here, we identified characterised novel, potentially colitogenic subset of Th17 intestine patients with Crohn's disease [CD]. Human expressing CCR5 ['pTh17'] co-expressed T-bet RORC/γt produced very high levels IL-17, together IFN-γ. They had gene signature effector distinct from established Th1/17 cells. pTh17 cells, but not associated intestinal...
Human CD4
Opioid drugs affect immunity, but not all opioid share the same immunomodulatory properties. Tapentadol is an analgesic drug with a dual synergistic mechanism of action: µ-opioid receptor agonism and noradrenaline reuptake inhibition. Weaker combined inhibition results in potent analgesia reduced side effects. We evaluated impact tapentadol on splenic cytokine normal hyperalgesia/allodynia mice, comparing it morphine reboxetine, inhibitor.Tapentadol, were injected subcutaneously into naïve...
Intracranial aneurysms (IAs) are very rare in children, and the characteristics of T-cells IA wall largely unknown. A comatose 7-years-old child was admitted to our center because a subarachnoid hemorrhage due ruptured giant aneurysm right middle cerebral artery. Two days after clipping patient fully awake with left hemiparesis. from peripheral blood this were analyzed by multi-dimensional flow cytometry. Unbiased analysis, based on use FlowSOM clustering dimensionality reduction technique...
Neurotoxicity is a common side effect of chemotherapeutics that often leads to the development chemotherapy-induced peripheral neuropathy (CIPN). The peptide Prokineticin 2 (PK2) has key role in experimental models CIPN and can be considered an insult-inducible endangering mediator. Since primary afferent sensory neurons are highly sensitive anticancer drugs, giving rise dysesthesias, aim our study was evaluate alterations induced by vincristine (VCR) bortezomib (BTZ) exposure neuron...
Abstract Recent research has shown that tau protein can be passed to neighboring cells, leading cellular senescence in the endothelial cells present central nervous system (CNS). This discovery could potentially open new doors for testing novel therapeutic compounds specifically target senescent (senolytics) or identifying biomarkers enable early detection of tauopathies and dementia.
Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used to treat migraine, but the mechanisms of their effects in this pathology not fully elucidated. The trigeminal ganglia and calcitonin gene-related peptide (CGRP) have been implicated pathophysiology migraine. release CGRP prostaglandin E 2 (PGE ) from freshly isolated rat was evaluated after oral administration nimesulide, etoricoxib, ketoprofen, NSAIDs with different pharmacological features. Thirty minutes administration, 10...
Summary So far it is thanks to antibiotics that illnesses, such as Tuberculosis (TB), are treatable. However, antimicrobial misuse combined with Mycobacterium tuberculosis phenotypic plasticity nullifying the effects of existing therapies. As a result, increasingly people dying (one person dies TB every 20 seconds), especially due rise multi-and extensively drug-resistant strains. There indeed urgent need for new and more effective therapies, which should match requirement avoiding in drug...
SARS-CoV-2 infections have been associated with the onset of thyroid disorders like classic subacute thyroiditis (SAT) or atypical SAT upon severe COVID disease (COV-A-SAT). Little is known about anti-viral immune responses. To define role T-cells in COV-A-SAT. from COV-A-SAT patients were analyzed by multi-dimensional flow cytometry, UMAP and DiffusionMap dimensionality reduction FlowSOM clustering. COVID-naïve healthy donors, autoimmune (ATD) following vaccination as controls. four eight...
ABSTRACT STAT3 is required for Th17-cell differentiation, and dominant-negative mutations cause Autosomal Dominant-Hyper-IgE Syndrome (AD-HIES), which characterized by a lack of Th17-cells recurrent infections with opportunistic extracellular pathogens. T-cell responses to pathogens in AD-HIES patients have however not been characterized. We previously identified an enigmatic population IL-10 producing CCR6 + Th-cells that were largely non-polarised lacked other subset-defining...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)