Fabrizio Ortolano
A5054357566- Traumatic Brain Injury and Neurovascular Disturbances
- Traumatic Brain Injury Research
- Trauma and Emergency Care Studies
- Cardiac Arrest and Resuscitation
- S100 Proteins and Annexins
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurosurgical Procedures and Complications
- Sepsis Diagnosis and Treatment
- Cerebrospinal fluid and hydrocephalus
- Immune Response and Inflammation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Complement system in diseases
- Intracranial Aneurysms: Treatment and Complications
- Immune cells in cancer
- Vitamin K Research Studies
- Thermal Regulation in Medicine
- Respiratory Support and Mechanisms
- Radiation Dose and Imaging
- Anesthesia and Neurotoxicity Research
- Long-Term Effects of COVID-19
- Infectious Encephalopathies and Encephalitis
- Optical Imaging and Spectroscopy Techniques
- Plant-based Medicinal Research
- Neuroscience and Neuropharmacology Research
- Intracerebral and Subarachnoid Hemorrhage Research
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2015-2025
University of Cambridge
2024
Addenbrooke's Hospital
2024
University of Groningen
2022
University Hospital of North Norway
2022
University Hospital Heidelberg
2021
Heidelberg University
2021
Charité - Universitätsmedizin Berlin
2020
Humboldt-Universität zu Berlin
2020
Freie Universität Berlin
2020
The studies on fractalkine and its unique receptor CX3CR1 in neurological disorders yielded contrasting results. We have explored the consequences of deletion ischemic (30′ MCAo) mice on: (1) brain infarct size; (2) microglia dynamism morphology; (3) expression markers microglia/macrophages (M/M) activation polarization. observed smaller infarcts cx3cr1 −/− (26.42 ± 7.41 mm 3 , mean sd) compared to wild type (36.29 11.57) −/+ (34.49 8.91) mice. longitudinally analyzed by vivo two‐photon...
Abstract Chronic post-concussive symptoms are common after mild traumatic brain injury (mTBI) and difficult to predict or treat. Thalamic functional integrity is particularly vulnerable in mTBI may be related long-term outcomes but requires further investigation. We compared structural MRI resting state 108 patients with a Glasgow Coma Scale (GCS) of 13–15 normal CT, 76 controls. examined whether acute changes thalamic connectivity were early markers for persistent explored neurochemical...
High intracranial pressure (HICP) may be a very early event after traumatic brain injury (TBI), but in most cases, especially when contusions and edema develop over time, HICP will worsen succeeding days. This study describes the incidence severity of elevated (ICP) TBI attempts to document its time course. In this prospective study, 201 patients whom ICP was monitored for more than 12 h were evaluated. measured, digitalized, analyzed manual filtering. The number episodes mean value every...
We investigated the occurrence of endotoxin (lipopolysaccharide, LPS) preconditioning in traumatic brain injury (TBI), evaluating time window LPS-induced protection, its persistence, and associated molecular mechanisms. Mice received 0.1 mg/kg LPS or saline intraperitoneally subsequently TBI (by controlled cortical impact injury) at various intervals. receiving 3, 5, 7 days before showed attenuated motor deficits 1 week after compared with mice saline. Those 5 had also a reduced contusion...
An impaired ability to regulate microglia activation by fractalkine (CX3CL1) leads chronic sub-activation. How this condition affects outcome after acute brain injury is still debated, with studies showing contrasting results depending on the timing and pathology. Here, we investigated early delayed consequences of receptor (CX3CR1) deletion neurological phenotypical features myeloid cells present in lesions mice traumatic (TBI). Wild type (WT) CX3CR1(-/-) C57Bl/6 were subjected sham or...
The incidence of Traumatic Brain Injury (TBI) is increasingly common in older adults aged ≥65 years, forming a growing public health problem. However, are underrepresented TBI research. Therefore, we aimed to provide an overview health-care utilization, and six-month outcomes after their determinants who sustained TBI.We used data from the prospective multi-center Collaborative European NeuroTrauma Effectiveness Research (CENTER-TBI) study. In-hospital post-hospital care utilization were...
The aim of the study was to evaluate effects C1-inhibitor (C1-INH), an endogenous inhibitor complement and kinin systems, on neurobehavioral histological outcome following controlled cortical impact brain injury.Experimental prospective randomized in mice.Experimental laboratory.Male C57Bl/6 mice (n = 81).Mice were subjected injury followed by intravenous bolus either C1-INH (15 U at 10 minutes or 1 hour postinjury) saline (equal volume, 150 microl postinjury). Sham-operated received...
The role of tumor necrosis factor (TNF) and its receptors after traumatic brain injury (TBI) remains unclear. We evaluated the effects genetic deletion either p55 or p75 TNF receptor on neurobehavioral outcome, histopathology, DNA damage apoptosis-related cell death/survival gene expression (bcl-2/bax), microglia/macrophage (M/M) activation in wild-type (WT) knockout mice TBI. Injured (-/-) showed a significant attenuation while worsening sensorimotor deficits compared with WT over 4 weeks...
Traumatic brain injury (TBI) is a leading cause of death and long-term disability worldwide. In addition to primary damage, systemic immune alterations occur, with evidence for dysregulated responses in aggravating TBI outcome complications. However, dysfunction following has been only partially understood, especially the elderly who represent substantial proportion patients worst outcome. Therefore, we aimed conduct an in-depth immunological characterization patients, by evaluating both...
Objective: Mannose-binding lectin protein is the activator of complement pathway. Goals were 1) to investigate mannose-binding expression after human and experimental traumatic brain injury induced by controlled cortical impact 2) evaluate whether deletion associated with reduced sequelae impact. Design: Translational research, combining a human/experimental observational study prospective study. Setting: University hospital/research laboratory. Patients Subjects: Brain-injured patients,...
Abstract Background After traumatic brain injury (TBI), fever is frequent. Brain temperature (BT), which directly linked to body temperature, may influence physiology. Increased and/or BT cause secondary damage, with deleterious effects on intracranial pressure (ICP), cerebral perfusion (CPP), and outcome. Methods Collaborative European NeuroTrauma Effectiveness Research in Traumatic Injury (CENTER-TBI), a prospective multicenter longitudinal study TBI Europe Israel, includes high resolution...
BackgroundEven patients with normal computed tomography (CT) head imaging may experience persistent symptoms for months to years after mild traumatic brain injury (mTBI). There is currently no good way predict recovery and triage who benefit from early follow-up targeted intervention. We aimed assess if existing prognostic models can be improved by serum biomarkers or diffusion tensor metrics (DTI) MRI, identify DTI.MethodsWe included 1025 aged >18 a Glasgow Coma Score >12 CT the...
Background: Aneurysmal Subarachnoid Haemorrhages (aSAH) are a severe condition often followed by vasospasm, causing several neurological and cognitive impairments in survivors. Nowadays there is still an open debate on actual impact of aSAH vasospasm over abilities time. This study aims at describing functioning focusing the acute phases after bleeding for 18 months, to investigate immediate long-term effects vasospasm. Methods: seventy adult patients have been prospectively recruited were...
The presence of intracranial hypertension (HICP) after traumatic brain injury (TBI) affects patient outcome. Intracranial pressure (ICP) data from electronic monitoring equipment are usually calculated and recorded hourly in the clinical chart by trained nurses. Little is known, however, about how precisely this method reflects real patterns ICP severe TBI. In study, we compared manual recording with a validated continuous computerized reference standard.Thirty randomly selected patients TBI...
To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging cells by multiphoton microscopy middle cerebral artery occlusion.Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled underwent permanent left distal occlusion electrocoagulation (n=6) or sham surgery and then laser 72 hours later.Extravasated number significantly increased versus sham. Two populations existed occlusion, possibly driven 2...
We explored the involvement of lectin pathway complement in post-traumatic brain injury (TBI) pathophysiology humans. Brain samples were obtained from 28 patients who had undergone therapeutic contusion removal, within 12 h (early) or >12 until five days (late) injury, and non-TBI patients. Imaging analysis indicated that initiator molecules (MBL, ficolin-1, ficolin-2 ficolin-3), key enzymes MASP-2 MASP-3, downstream components (C3 fragments TCC) present inside outside vessels all...
The c-Jun N-terminal kinase (JNK) pathway is involved in cell stress and apoptosis. We tested the hypothesis that this plays a role traumatic brain injury (TBI) by assessing JNK activation human tissues brains of mice subjected to controlled cortical impact injury. also assessed effects specific inhibition cell-permeable inhibitor peptide, D-JNKI1, on neurobehavioral function posttraumatic loss mice. was administered intraperitoneally 10 minutes after showed robust both contusion specimens...
We describe the case of a 63-year-old woman who developed coronavirus disease 2019-associated acute encephalopathy with perivascular gadolinium enhancement.
Abstract Background Magnetic resonance imaging (MRI) carries prognostic importance after traumatic brain injury (TBI), especially when computed tomography (CT) fails to fully explain the level of unconsciousness. However, in critically ill patients, risk deterioration during transfer needs be balanced against benefit detecting prognostically relevant information on MRI. We therefore aimed assess if day serum protein biomarkers could identify TBI patients whom risks are compensated by...