A5087715933- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Muscle Physiology and Disorders
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Congenital heart defects research
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Viral-associated cancers and disorders
- Occupational and environmental lung diseases
- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Sarcoma Diagnosis and Treatment
- Lipid metabolism and disorders
- Nanoplatforms for cancer theranostics
- Adipose Tissue and Metabolism
University of Milan
2021-2024
University of Parma
2009-2024
University of Brescia
1999-2022
Fondazione IRCCS Istituto Neurologico Carlo Besta
2012-2021
Institute of Neurological Sciences
2014-2020
Multidisciplinary Digital Publishing Institute (Switzerland)
2019
Institute for the Chemistry of OrganoMetallic Compounds
2019
Sapienza University of Rome
2019
John Wiley & Sons (United States)
2018
University of Turin
1990-2015
We investigated in vitro the properties of selected populations cancer stem-like cells defined as tumorospheres that were obtained from human glioblastoma. also assessed their potential and capability differentiating into mature central nervous system. In vivo, tumorigenicity was confirmed after transplantation brain non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. The angiogenic glioblastoma-derived grown adherent revealed by evaluating release factors such vascular...
We evaluated the healing potential of human fetal aorta-derived CD133 + progenitor cells and their conditioned medium (CD133 CCM) in a new model ischemic diabetic ulcer. Streptozotocin-induced mice underwent bilateral limb ischemia wounding. One wound was covered with collagen containing 2×10 4 or − vehicle. The contralateral wound, only collagen, served as control. Fetal expressed high levels wingless ( Wnt ) genes, which were downregulated following differentiation into along upregulation...
Ceruloplasmin, Copper Ions, and Angiogenesis Get access Katari S. Raju, Raju Search for other works by this author on: Oxford Academic PubMed Google Scholar Giulio Alessandri, Alessandri Marina Ziche, Ziche Pietro M. Gullino 4 4Address reprint requests to Dr. Gullino, Building 10, Room 5B36, National Institutes of Health, Bethesda, Md. 20205. JNCI: Journal the Cancer Institute, Volume 69, Issue 5, November 1982, Pages 1183–1188, https://doi.org/10.1093/jnci/69.5.1183 Published: 01 1982...
Background: Malignant glial tumors, including glioblastoma multiforme, account for 15 – 20% of pediatric CNS malignancies. They are most resistant to therapy and associated with a poor prognosis.Objective: Given the ability mesenchymal stem cells (MSCs) affect glioma growth, we investigated effects extracellular vesicles (EVs) derived from MSCs on U87MG line.Methods: EVs were isolated culture media different sources, bone marrow (BM), umbilical cord (UC) adipose tissue (AT) added culture....
Mesenchymal stromal cells may represent an ideal candidate to deliver anti-cancer drugs. In a previous study, we demonstrated that exposure of mouse bone marrow derived Doxorubicin led them acquire anti-proliferative potential towards co-cultured haematopoietic stem (HSCs). We thus hypothesized whether freshly isolated human (hMSCs) and mature murine (SR4987 line) primed in vitro with drugs then localized near cancer cells, could inhibit proliferation.Paclitaxel (PTX) was used prime culture...
Mesenchymal stem cells (MSCs) are multipotent able to differentiate into different cell lineages. However, MSCs represent a subpopulation of more complex composition stroma contained in mesenchymal tissue. Due lack specific markers, it is difficult distinguish from other mature stromal such as fibroblasts, which, conversely, abundant In order find distinguishing features between and we studied the phenotypic functional human adipose-derived (AD-MSCs) side by with normal dermal fibroblasts...
Abstract Introduction Silk fibroin (SF) scaffolds have been shown to be a suitable substrate for tissue engineering and improve regeneration when cellularized with mesenchymal stromal cells (MSCs). We here demonstrate, the first time, that electrospun nanofibrous SF patches human adipose-derived MSCs (Ad-MSCs-SF), or decellularized (D-Ad-MSCs-SF), are effective in treatment of skin wounds, improving db/db diabetic mice. Methods The conformational structural analyses D-Ad-MSCs-SF were...
Pericytes play an important role in modulating angiogenesis, but the origin of these cells is poorly understood. To evaluate whether mature vessel wall contains pericyte progenitor cells, nonendothelial mesenchymal isolated from rat aorta were cultured a serum-free medium optimized for stem cells. This method led to isolation anchorage-independent that proliferated slowly suspension, forming spheroidal colonies. process required basic fibroblast growth factor (bFGF) culture medium, because...
Mesenchymal stromal cells (MSCs), as advanced therapy products, must satisfy all the requirements for human use of medicinal aiming to maintain quality and safety cells. The MSC manufacturing process clinical should comply with principles Good Manufacturing Practice (GMP). This ensures that cell preparations are produced controlled, from collection manipulation raw materials, through processing intermediate controls, storage, labeling packaging, release. objective this document is provide...
Vascular diseases supported by aberrant angiogenesis have increased incidence in HIV-1-infected patients. Several data suggest that endothelium dysfunction relies on action of HIV-1 proteins rather than a direct effect the virus itself. The matrix protein p17 is known to deregulate biological activity different immune cells. Recently, was found mimic IL-8 chemokine binding receptor CXCR1. Here we show binds with high affinity CXCR2, CXCR1-related receptor, and promotes formation...
Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for advanced cell-based therapies. They routinely obtained enzymatically from fat lipoaspirate (LP) as SVF, and may undergo prolonged ex vivo expansion, with significant senescence decline in multipotency. Besides, these techniques have complex regulatory issues, thus incurring the compelling requirements of GMP guidelines. Hence, availability minimally manipulated, autologous adipose tissue would remarkable biomedical...
Summary Current leukaemia therapy focuses on increasing chemotherapy efficacy. Mesenchymal stromal cells ( MSC s) have been proposed for carrying and delivery drugs to improve killing of cancer cells. We shown that s loaded with Paclitaxel PTX ) acquire a potent anti‐tumour activity. investigated the effect human hMSC mouse SR 4987 hMSCsPTX MOLT ‐4 L 1210, two cell LCs lines origin, respectively. showed strong anti‐LC , co‐injected or intra‐tumour injected into established subcutaneous...
Mesenchymal stem/stromal cells (MSCs) represent an attractive tool for cell-based cancer therapy mainly because of their ability to migrate tumors and release bioactive molecules. However, the impact MSCs on tumor growth has not been fully established. We previously demonstrated that murine show a strong tropism towards glioblastoma (GBM) brain xenografts these are able uptake chemotherapeutic drug paclitaxel (PTX), maintaining tumor. Here, we address therapy-relevant issue using from human...
Abstract Human mesenchymal stromal cells (MSCs) have been widely investigated both for regenerative medicine and their antinflammatory/immunomodulatory capacity. However, ability to home pathological tissues suggested the development of strategies using MSCs as carrier deliver drug into tumor microenvironment. obtained from different can be loaded in vitro with anti-cancer drugs by a simple procedures. In this report, we studied isolated expanded gingival papilla (GinPa-MSCs), testing uptake...
In the context of drug delivery, mesenchymal stromal cells (MSCs) from bone marrow and adipose tissue have emerged as interesting candidates due to their homing abilities capacity carry toxic loads, while at same time being highly resistant effects. Amongst many sources MSCs which been identified, human term placenta has attracted particular interest its unique, tissue-related characteristics, including high cell yield virtually absent expression leukocyte antigens co-stimulatory molecules....
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, characterized by studying their secretome before after priming with paclitaxel (PTX). The did not affect the growth cancer cell lines tested vitro, whereas primed (GinPaMSCs/PTX) exerted a significant anticancer effect. able uptake then release amounts pharmacologically effective against cells, as demonstrated...
Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in closed system device, has been successfully used aesthetic medicine as well orthopedic general surgery. Interestingly, diseases, this ready-to-use cell derivative seems to have prolonged time efficacy even upon single shot injection into osteoarthritic tissues. Here, we investigated...