A5042626370- Spectroscopy Techniques in Biomedical and Chemical Research
- Protein Structure and Dynamics
- Spectroscopy and Chemometric Analyses
- Alzheimer's disease research and treatments
- thermodynamics and calorimetric analyses
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Protein purification and stability
- Proteins in Food Systems
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Endoplasmic Reticulum Stress and Disease
- Metabolomics and Mass Spectrometry Studies
- Heat shock proteins research
- Viral Infectious Diseases and Gene Expression in Insects
- Analytical Chemistry and Chromatography
- Microbial Inactivation Methods
- Synthesis and properties of polymers
- Bee Products Chemical Analysis
- Fibroblast Growth Factor Research
- Protein Interaction Studies and Fluorescence Analysis
- Biofuel production and bioconversion
- Transgenic Plants and Applications
University of Milano-Bicocca
2015-2025
National Interuniversity Consortium for the Physical Sciences of Matter
2006-2016
University of Milan
2000-2014
Italian Institute of Technology
2013
Center for Nanotechnology Innovation
2013
Policlinico San Matteo Fondazione
2011
Istituti di Ricovero e Cura a Carattere Scientifico
2011
IFOM
2008-2009
Istituto Nazionale per la Fisica della Materia
2004-2005
ABSTRACT Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one leading causes nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] Raman) could provide potential alternatives to conventional typing methods, because they are fast, easy perform, economical. We present a comparative study using phenotypic, genotypic, vibrational for collection 18 Enterococcus...
ABSTRACT Fourier transform infrared and Raman microspectroscopy are currently being developed as new methods for the rapid identification of clinically relevant microorganisms. These involve measuring spectra from microcolonies which have been cultured little 6 h, followed by nonsubjective microorganisms through use multivariate statistical analyses. To examine biological heterogeneity microorganism growth is reflected in spectra, measurements were acquired various positions within...
The secondary structure of lipase 1 from Candida rugosa, a model system for large monomeric enzymes, has been studied by FTIR (Fourier-transform infrared) spectroscopy in water and 2H2O. content, determined the analysis amide I band absorption through second derivative curve fitting procedures, is agreement with that estimated X-ray data predicts, addition, existence two classes α-helices. We have also investigated enzyme stability aggregation at high temperature following protein unfolding....
The production of synthetic plastics, especially polyethylene, has reached a crucial level, making highly challenging plastic waste management. This paper aims to elucidate the role two novel laccase-like multicopper oxidases in polyethylene oxidative degradation based on transcriptomic data from Rhodococcus opacus R7 grown polyethylene. purification recombinant proteins, LMCO2 and LMCO3 belonging respectively three- two-domains laccase families, showed that they are endowed with different...
AL amyloidosis is a life-threatening disease caused by deposition of immunoglobulin light chains. Whilst the mechanisms underlying chains amyloidogenesis in vivo remain unclear, several studies have highlighted role that tissue environment and structural amyloidogenicity individual pathogenesis.AL natural deposits contain both full-length fragments encompassing variable domain (VL) as well different length segments constant region (CL), thus highlighting relevance proteolysis may...
Neural cell adhesion molecule (NCAM) associates with fibroblast growth factor (FGF) receptor-1 (FGFR1). However, the biological significance of this interaction remains largely elusive. In study, we show that NCAM induces a specific, FGFR1-mediated cellular response is remarkably different from elicited by FGF-2. contrast to FGF-induced degradation endocytic FGFR1, promotes stabilization receptor, which recycled surface in Rab11- and Src-dependent manner. turn, FGFR1 recycling required for...
We describe the rapid isolation of single-domain recombinant antibodies in VHH format from a pre-immune llama library created our laboratory. Such naïve has demonstrated to be versatile tool and enabled several different for any six proteins panned parallel. The binders specific human fibroblast growth factor receptor 1 (FGFR1) were successively analyzed more detail resulted suitable both western blot immunofluorescence analyses. Several milligrams per liter purified by affinity...
Oleaginous microorganisms, such as different yeast and algal species, can represent a sustainable alternative to plant oil for the production of biodiesel. They accumulate fatty acids (FA) up 70% their dry weight with predominance (mono)unsaturated similarly what plants do, but differently from animals. In addition, growth is not in competition either food, feed crops, or agricultural land.Despite these advantages, exploitation single cell system still at an early developmental stage....
Microbial lipids can represent a valuable alternative feedstock for biodiesel production in the context of viable bio-based economy. This be driven by cultivating some oleaginous microorganisms on crude-glycerol, 10 % (w/w) by-product produced during transesterification process from oils into biodiesel. Despite attractive, perspective is still economically unsustainable, mainly because impurities crude glycerol negatively affect microbial performances. In this view, selection best cell...
Abstract Light chain (AL) amyloidosis, caused by deposition of amyloidogenic immunoglobulin light chains (LCs), is the most common systemic form in industrialized countries. Still open questions, and premises for developing targeted therapies, concern mechanisms amyloid formation vivo bases organ targeting dysfunction. Investigating material its natural environment crucial to obtain new insights on molecular features fibrillar deposits at individual level. To this aim, we used Fourier...
Abstract Plastic waste management has become a global issue. Polyethylene (PE) is the most abundant synthetic plastic worldwide, and one of resistant to biodegradation. Indeed, few bacteria can degrade polyethylene. In this paper, transcriptomic analysis unveiled for first time Rhodococcus opacus R7 complex genetic system based on diverse oxidoreductases polyethylene The RNA-seq allowed uncovering genes putatively involved in step oxidation. In-depth investigations through preliminary...
The aggregation of a recombinant lipase as inclusion bodies (IBs) was studied directly within intact Escherichia coli cells by FT‐IR microspectroscopy. Through this approach, it possible to monitor in real time the different kinetics IB formation at 37 and 27 °C, excellent agreement with results SDS–PAGE analysis. Furthermore, insights on residual native‐like structure expressed protein – both isolated inside were obtained secondary analysis Amide I band spectra.
Broadly used in biocatalysis as acyl acceptors or (co)-solvents, short-chain alcohols often cause irreversible loss of enzyme activity. Understanding the mechanisms inactivation is a necessary step toward optimization biocatalytic reactions and design enzyme-based sustainable processes. The functional structural responses an immobilized enzyme, Novozym 435 (N-435), exposed to methanol, ethanol, tert-butanol, are explored this work. N-435 consists Candida antarctica lipase B (CALB) adsorbed...
This study investigates the extraction, characterization, and cosmetic application of silk sericin, a protein derived from Bombyx mori silkworm cocoons, with focus on its potential in sustainable biodegradable formulations. Sericin was extracted using high-temperature, high-pressure autoclave degumming method spray-dried into stable powder. The molecular weight distribution sericin analyzed, revealing fractions ranging 10 to 37 kDa Elution 1A 25–40 1B. Electrospinning led increased β-sheet...
In this work the effect of betaine on structure and aggregation GST-GFP fluorescent fusion protein was studied by different complementary techniques, including electron microscopy, dynamic light scattering, circular dichroism, FTIR spectroscopy. Although osmolytes are known to be stabilizers in vivo, our model found strictly concentration dependent. We demonstrated that, changing concentration, it possible tune formation soluble assemblies insoluble aggregates, as well disaggregate preformed...
Abstract Protein misfolding and aggregation are associated with a number of human degenerative diseases. In spite the enormous research efforts to develop effective strategies aimed at interfering pathogenic cascades induced by misfolded/aggregated peptides/proteins, necessary detailed understanding molecular bases amyloid formation toxicity is still lacking. To this aim, approaches able provide global insight in amyloid-mediated physiological alterations importance. study, we exploited...
Lacrimal fluid is an attractive source of noninvasive biomarkers, the main limitation being small sample amounts typically collected. Advanced analytical methods to allow for proteomics profiling from a few microliters are needed develop innovative with perspectives applications precision medicine. This work describes effective, pipeline single-tear analysis by ultrahigh-resolution, shotgun 23 healthy human volunteers, leading high-confidence identification total 890 proteins. Highly...