A5028962045- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fermentation and Sensory Analysis
- Algal biology and biofuel production
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Protein Kinase Regulation and GTPase Signaling
- Yeasts and Rust Fungi Studies
- Wastewater Treatment and Nitrogen Removal
- RNA Research and Splicing
- Plant biochemistry and biosynthesis
- biodegradable polymer synthesis and properties
- Enzyme-mediated dye degradation
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Fungal Biology and Applications
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
University of Milano-Bicocca
2016-2025
University of Palermo
2023
University of Milan
1997-2002
Abstract Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for controlled production foreign proteins scientific, pharmacological or industrial interest. A major obstacle protein processes a limit to overall success has abundance misfolded polypeptides, which fail reach their native conformation. The presence folding-reluctant causes considerable stress host cells. characterization such adverse conditions elicited cell...
The sustainable production of biofuels remains one the major issues upcoming years. Among number most desirable molecules to be produced, butanol and isobutanol deserve a prominent place. They have superior liquid-fuel features in respect ethanol. Particularly, has similar properties gasoline thus it potential used as substitute for currently running engines. Clostridia are recognized natural good producers employed industrial-scale solvents. Due their complex metabolic characteristics...
ABSTRACT Yeast strains expressing heterologous l -lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, acid exerts a high level stress on the cells. In present study we analyzed intracellular pH (pH i ) and viability by staining with cSNARF-4F ethidium bromide, respectively, two lactic-acid-producing Saccharomyces cerevisiae , CEN.PK m850 RWB876. The results showed strain producing more acid, m850, has...
Oleaginous microorganisms, such as different yeast and algal species, can represent a sustainable alternative to plant oil for the production of biodiesel. They accumulate fatty acids (FA) up 70% their dry weight with predominance (mono)unsaturated similarly what plants do, but differently from animals. In addition, growth is not in competition either food, feed crops, or agricultural land.Despite these advantages, exploitation single cell system still at an early developmental stage....
Microbial lipids can represent a valuable alternative feedstock for biodiesel production in the context of viable bio-based economy. This be driven by cultivating some oleaginous microorganisms on crude-glycerol, 10 % (w/w) by-product produced during transesterification process from oils into biodiesel. Despite attractive, perspective is still economically unsustainable, mainly because impurities crude glycerol negatively affect microbial performances. In this view, selection best cell...
Many interspecies hybrids have been discovered in yeasts, but most of these are asexual and can replicate only mitotically. Whole-genome duplication has proposed as a mechanism by which regain fertility, restoring their ability to perform meiosis sporulate. Here, we show that this process occurred naturally during the evolution Zygosaccharomyces parabailii, an hybrid was formed mating between 2 parents differed 7% genome sequence many interchromosomal rearrangements. Surprisingly, Z....
In Saccharomyces cerevisiae the subcellular distribution of Bcy1 is carbon source dependent. glucose-grown cells, almost exclusively nuclear, while it appears more evenly distributed between nucleus and cytoplasm in source-derepressed cells. Here we show that phosphorylation its N-terminal domain directs to cytoplasm. Biochemical fractionation revealed cytoplasmic fraction contains mostly phosphorylated Bcy1, whereas unmodified predominantly present nuclear fraction. Site-directed...
ABSTRACT Intracellular pH has an important role in the maintenance of normal functions yeast cells. The ability cell to maintain this homeostasis also response environmental changes gained more and interest both basic applied research. In study we describe a protocol which allows rapid determination intracellular Saccharomyces cerevisiae method is based on flow cytometry employs pH-dependent fluorescent probe carboxy SNARF-4F. attempts minimize perturbation system under study, thus leading...
In industrial large scale bio-reactions micro-organisms are generally exposed to a variety of environmental stresses, which might be detrimental for growth and productivity. Reactive oxygen species (ROS) play key role among the common stress factors--directly--through incomplete reduction O(2) during respiration, or indirectly--caused by other stressing factors. Vitamin C L-ascorbic acid acts as scavenger ROS, thereby potentially protecting cells from harmful oxidative products. While most...
Metabolic pathway manipulation for improving the properties and productivity of microorganisms is becoming a well established concept. For production important metabolites, but also better understanding fundamentals cell biology, detailed studies are required. In this work we analysed lactate from metabolic engineered Saccharomyces cerevisiae cells expressing heterologous dehydrogenase (LDH) gene. The LDH gene expression in budding yeast introduces novel alternative NAD+ regeneration,...
The yeast Kluyveromyces marxianus features specific traits that render it attractive for industrial applications. These include production of ethanol which, together with thermotolerance and the ability to grow a high growth rate on wide range substrates, could make an alternative Saccharomyces cerevisiae as producer. However, its co-ferment C5 C6 sugars under oxygen-limited conditions is far from being fully characterized. In present study, K. CBS712 strain was cultivated in defined medium...
Research on bioconversion based insects is intensifying as it addresses the problem of reducing and reusing food industrial waste. To reach this goal, we need to find more means pairing waste insects. With brewers' spent grains (BSG) - a brewing industry paired with oleaginous biomass thraustochytrid Schizochytrium limacinum cultivated crude glycerol major biodiesel production were successfully used grow Hermetia illucens larvae. Combining BSG S. in diet an attempt design lipid profile H....
ABSTRACT Yeasts do not possess an endogenous biochemical pathway for the synthesis of vitamin C. However, incubated with l -galactose, -galactono-1,4-lactone, or -gulono-1,4-lactone intermediates from plant animal leading to -ascorbic acid, Saccharomyces cerevisiae and Zygosaccharomyces bailii cells accumulate intracellularly. Overexpression S. enzymes d -arabinose dehydrogenase -arabinono-1,4-lactone oxidase enhances this ability significantly. In fact, respective recombinant yeast strains...
Microorganisms encounter diverse stress conditions in their native habitats but also during fermentation processes, which have an impact on industrial process performance. These environmental stresses and the physiological reactions they trigger, including changes protein folding/secretion machinery, are highly interrelated. Thus, investigation of factors, influence expression secretion is still great importance. Among all possible stresses, temperature appears particularly important for...
Abstract Background Saccharomyces cerevisiae and Pichia pastoris are two of the most relevant microbial eukaryotic platforms for production recombinant proteins. Their known genome sequences enabled several transcriptomic profiling studies under many different environmental conditions, thus mimicking not only perturbations adaptations which occur in their natural surroundings, but also industrial processes. Notably, majority such transcriptome analyses were performed using non-engineered...
The industrial relevance of organic acids is high; because their chemical properties, they can be used as building blocks well single-molecule agents with a huge annual market. Organic acid platforms derive from fossil sources by petrochemical refining processes, but most them also represent natural metabolites produced many cells. They are the products, by-products or co-products primary metabolic processes microbial Thanks to potential cell factories and development biotechnology, last...