A5049100353- Cancer, Hypoxia, and Metabolism
- Alzheimer's disease research and treatments
- Cancer, Lipids, and Metabolism
- Cardiovascular Function and Risk Factors
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Amyotrophic Lateral Sclerosis Research
- Protein Kinase Regulation and GTPase Signaling
- Angiogenesis and VEGF in Cancer
- Nanoparticles: synthesis and applications
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Trace Elements in Health
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac Ischemia and Reperfusion
- Cardiac electrophysiology and arrhythmias
- Mesenchymal stem cell research
- Prion Diseases and Protein Misfolding
- Cardiac Arrest and Resuscitation
- Cholinesterase and Neurodegenerative Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Extracellular vesicles in disease
- Heart Failure Treatment and Management
- Hormonal Regulation and Hypertension
- Nanoparticle-Based Drug Delivery
- ATP Synthase and ATPases Research
Mario Negri Institute for Pharmacological Research
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
Laboratoire d’Imagerie Biomédicale
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2014
New York Medical College
2000-2005
University of Milano-Bicocca
2004-2005
University of Milan
2004-2005
Sapienza University of Rome
2004-2005
Molecular Oncology (United States)
2005
European Institute of Oncology
2005
Cardiac myocytes have been traditionally regarded as terminally differentiated cells that adapt to increased work and compensate for disease exclusively through hypertrophy. However, in the past few years, compelling evidence has accumulated suggesting heart regenerative potential. Recent studies even surmised existence of resident cardiac stem cells, endothelial generating cardiomyocytes by cell contact or extracardiac progenitors cardiomyocytes, but these findings are still controversial....
The cytokine erythropoietin (Epo) is tissue-protective in preclinical models of ischemic, traumatic, toxic, and inflammatory injuries. We have recently characterized Epo derivatives that do not bind to the receptor (EpoR) yet are tissue-protective. For example, carbamylated (CEpo) does stimulate erythropoiesis, it prevents tissue injury a wide variety vivo vitro models. These observations suggest another responsible for actions Epo. Notably, prior investigation suggests EpoR physically...
Amyotrophic lateral sclerosis is the most common motor neuron disease and still incurable. The mechanisms leading to selective vulnerability are not known. interplay between neurons astrocytes crucial in outcome of disease. We show that mutant copper-zinc superoxide dismutase (SOD1) overexpression primary astrocyte cultures associated with decreased levels proteins involved secretory pathways. This linked a general reduction total secreted proteins, except for specific enrichment number...
Engineered nanoparticles offer the chance to improve drug transport and delivery through biological barriers, exploiting possibility leave blood circulation traverse endothelial vascular bed, blood–brain barrier (BBB) included, reach their target. It is known that gather molecules on surface upon contact with fluids, forming "protein corona", which can affect fate therapeutic/diagnostic performance, yet no information corona's evolution across has been gathered so far. Using a cellular model...
Stimulation of the local renin-angiotensin system and apoptosis characterize diabetic heart. Because IGF-1 reduces angiotensin (Ang) II apoptosis, we tested whether streptozotocin-induced cardiomyopathy was attenuated in transgenic mice (TGM). Diabetes progressively depressed ventricular performance wild-type (WTM) but had no hemodynamic effect on TGM. Myocyte measured at 7 30 days after onset diabetes twofold higher WTM than necrosis apparent only more severe WTM. Diabetic nontransgenic...
To determine whether enzymatic p53 glycosylation leads to angiotensin II formation followed by phosphorylation, prolonged activation of the renin-angiotensin system, and apoptosis, ventricular myocytes were exposed levels glucose mimicking diabetic hyperglycemia. At a high concentration, O-glycosylation occurred between 10 20 min, reached its peak at 1 h, then decreased with time. Angiotensin synthesis increased 45 min resulting in p38 mitogen-activated protein (MAP) kinase–driven...
The cytokine erythropoietin (EPO) protects the heart from ischemic injury, in part by preventing apoptosis. However, EPO administration can also raise hemoglobin concentration, which, increasing oxygen delivery, confounds assignment of cause and effect. availability analogs that do not bind to dimeric receptor lack erythropoietic activity, e.g., carbamylated (CEPO), provides an opportunity determine whether possesses direct cardioprotective activity. In vivo , cardiomyocyte loss after...
Alzheimer's disease is characterized by the accumulation and deposition of plaques β-amyloid (Aβ) peptide in brain. Given its pivotal role, new therapies targeting Aβ are demand. We rationally designed liposomes brain promoting disaggregation assemblies evaluated their efficiency reducing burden mouse models. Liposomes were bifunctionalized with a derived from apolipoprotein-E receptor-binding domain for blood–brain barrier phosphatidic acid binding. Bifunctionalized display unique ability...
Much evidence shows that acute and chronic inflammation in spinal cord injury (SCI), characterized by immune cell infiltration release of inflammatory mediators, is implicated development the secondary phase occurs after trauma worsening damage. Activation microglia/macrophages associated response appears to be a self-propelling mechanism leads progressive neurodegeneration persisting pain state. Recent advances polymer science have provided huge amount innovations leading increased interest...
Traumatic brain injury is a risk factor for subsequent neurodegenerative disease, including chronic traumatic encephalopathy, tauopathy mostly associated with repetitive concussion and blast, but not well recognized as consequence of severe injury. Here we show that single trauma the emergence widespread hyperphosphorylated tau pathology in proportion humans surviving late after In parallel experimental studies, model wild-type mice, found progressive pathology, replicating findings humans....
Podocyte injury leading to albuminuria is a characteristic feature of diabetic nephropathy (DN). Hyperglycemia and advanced glycation end products (AGEs) are major determinants DN. However, the underlying mechanisms podocyte remain poorly understood. The cytosolic protein TNFAIP2/M-Sec required for tunneling nanotubes (TNTs) formation, which membrane channels that transiently connect cells, allowing organelle transfer. Podocytes express TNFAIP2 form TNTs, but potential relevance TNFAIP2-TNT...
This review focuses on the correlation between physico-chemical characteristics of TiO 2 nanoparticles, their passage through biological barriers, and impact this nanomaterial human environmental health.
Systemic light chain (LC) amyloidosis (AL) is a disease where organs are damaged by an overload of misfolded patient-specific antibody-derived LC, secreted abnormal B cell clone. The high LC concentration in the blood leads to amyloid deposition at organ sites. Indeed, cryogenic electron microscopy (cryo-EM) has revealed unique folds for heart-derived fibrils taken from different patients. Here, we present cryo-EM structure AL (AL59) another patient with severe cardiac involvement....
Abstract Macrophages are crucial drivers of innate immunity. Reprogramming macrophages to a restorative phenotype in cancer or autoimmune diseases can stop their cancer‐promoting activity trigger anti‐inflammatory Glycans have emerged as key components for immunity they involved many pathophysiological disorders. Previous studies demonstrated that supraphysiological amounts mannose (Man) sialic acid (Sia) inhibit tumor growth and stimulate differentiation regulatory T cells. Man is known...
Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or heart upon ischemia-reperfusion injury. The defect was not observed mice with endothelium-restricted deficiency but still detectable transplanted bone marrow from -/- donors. Microscopic examination mesenteric microvasculature showed...
Fatal familial insomnia (FFI) and a genetic form of Creutzfeldt-Jakob disease (CJD178) are clinically different prion disorders linked to the D178N protein (PrP) mutation. The phenotype is determined by 129 M/V polymorphism on mutant allele, which thought influence PrP misfolding, leading formation distinctive strains with specific neurotoxic properties. However, mechanism misfolded variants cause diseases not known. We generated transgenic (Tg) mice expressing mouse homolog FFI These...
Titanium dioxide (TiO2) is widely used in pharmaceuticals preparations, cosmetics, and as a food additive (E171). It contains microparticles fraction of nanoparticles (NPs) which can be absorbed systemically by humans after ingestion. Increasing concern has been aroused about the impact oral exposure to TiO2 NPs from dietary non-dietary sources on human health. In spite several toxicological studies conducted recent years, solid risk assessment E171 not satisfactorily achieved. We...