A5015719345- Amyloidosis: Diagnosis, Treatment, Outcomes
- Alzheimer's disease research and treatments
- Soft tissue tumors and treatment
- Vascular Tumors and Angiosarcomas
- Intracerebral and Subarachnoid Hemorrhage Research
- Lysosomal Storage Disorders Research
- Medical Imaging and Pathology Studies
- Cardiomyopathy and Myosin Studies
- Dermatologic Treatments and Research
- Soft tissue tumor case studies
- Transplantation: Methods and Outcomes
- Glycogen Storage Diseases and Myoclonus
- Skin Diseases and Diabetes
- Porphyrin Metabolism and Disorders
- Neurogenetic and Muscular Disorders Research
- Cellular transport and secretion
- Optical Coherence Tomography Applications
- Vascular Malformations and Hemangiomas
- Atherosclerosis and Cardiovascular Diseases
- Genomics and Rare Diseases
- Muscle Physiology and Disorders
- COVID-19 epidemiological studies
- Parathyroid Disorders and Treatments
- Mechanical Circulatory Support Devices
- Occupational and environmental lung diseases
Istituti di Ricovero e Cura a Carattere Scientifico
2003-2024
Policlinico San Matteo Fondazione
2003-2024
University of Genoa
2023
Foundation University Islamabad
2020
Center for Inherited Blood Disorders
2015
University of Pavia
1996-2011
AL amyloidosis is a life-threatening disease caused by deposition of immunoglobulin light chains. Whilst the mechanisms underlying chains amyloidogenesis in vivo remain unclear, several studies have highlighted role that tissue environment and structural amyloidogenicity individual pathogenesis.AL natural deposits contain both full-length fragments encompassing variable domain (VL) as well different length segments constant region (CL), thus highlighting relevance proteolysis may...
We evaluated the role of electron microscopy and immuno-electron studies on abdominal fat fine-needle biopsy samples in diagnosis characterization cardiac amyloidosis.The series consists 15 patients with echocardiographic evidence "restrictive cardiomyopathy" suspected to be due amyloidosis. Patients underwent: clinical examination, electrocardiography, 2-D Doppler echocardiography, immunofixation serum urine for detection monoclonal immunoglobulins, biopsies that were investigated polarized...
Vulnerable plaques are characterized by a high macrophage content. We investigated the optical coherence tomography (OCT) capability of identifying coronary plaque presence using tissue property indexes.Fifteen epicardial arteries were imaged OCT and subsequently analysed histology. Correlating OCT-histological sections identified regions interest (ROIs) selected on both atherosclerotic normal appearing vessel tracts. OCT-derived indexes named normalized standard deviation (NSD), signal...
Abstract Objective . To examine the ultrastructure of human synovial mast cells in situ, to identify immunologic and nonimmunologic stimuli that activate these vitro, quantify a number preformed de novo–synthesized mediators. Methods We conducted an ultrastructural study situ performed immunoelectron microscopy localization tryptase chymase. Isolated were analyzed biochemically, immunologically, functionally vitro compared with from lung, heart, skin. Results Ultrastructural tissue revealed...
We aimed to explore whether variants of SARS-CoV-2 (Chinese-derived strain (D614, lineage A), Italian PV10734 (D614G, B.1.1) and Alpha (lineage B.1.1.7)) were able infect monocytes (MN) monocyte-derived macrophages (MDM) these infected cells may, in turn, be vectors infection. For this purpose, we designed an vitro study following the evolution MN MDM infection at different time points order confirm permissive for replication. Finally, investigated whether, regardless viral replication,...
The present study reports our optimized fixation and processing methods for the light electron microscopic immunohistochemical characterization of tissue amyloid. involved a series 25 abdominal fat aspirates 11 biopsy samples from different organs 36 patients. We tested short method enzymatic, acidic physical (microwave oven) pretreatments on paraffin slides microscopy, Congo red stain immunohistochemistry. Microwave antigen retrieval provided highest enhancement both reactions with all...
Tacrolimus (TAC) is an immunosuppressant drug approved both in the US and EU, widely used for prophylaxis of organ rejection after transplantation. This a critical dose drug: low levels whole blood can lead to exposure high risk acute rejection, whereas overexposure puts patients at toxicity infection. Both situations occur whole-blood concentrations considered be within narrow TAC therapeutic range. We assumed poor correlation between trough incidence rejection; therefore, we propose study...
Glomuvenous malformations (OMIM 138000) are hamartomas presenting in childhood as multiple, bluish papules and nodules the skin, which characterized histopathologically by irregular vascular spaces surrounded typical glomus cells. caused autosomal dominant mutations of GLMN gene. A 34-year-old woman her 16-year-old son presented with since childhood. Biopsy specimens from both patients showed histopathologic features glomuvenous malformations, unusually consistent close association smooth...
Abstract This paper is an report of the investigation in vitro binding 99m Tc-DPD for synthetic amyloid fibrils used diagnosis cardiac amyloidosis (CA), as compared with use Tc-HMDP and 99mTc-PPI. It also includes inquiry into role played by Ca 2+ ions serum proteins on to like materials, well saturability specificity DPD versus amorphous precipitates (AP). In work, insulin (SIF) AP were characterized Congo red staining TEM imaging. An equal amount three radiopharmaceuticals then added...
Xeroderma pigmentosum (XP) is a rare autosomal recessive disease characterized by severe cutaneous and ocular sensitivity to sunlight, leading skin cancer. Most XP patients belong the complementation groups (XP-A XP-G), due mutations in genes involved nucleotide excision repair (NER). On other hand, Variant type (XP-V, OMIM#278750), which accounts for about 20% of all patients, associated with normal NER function. The gene POLH, encodes polymerase η (pol η) allowing translesion synthesis...
Bateman’s purpura is a disease characterized by the presence of hemorrhagic non-inflammatory patches variable size, sometimes associated with disepithelisation, which arise in context chronically sun exposed skin elderly. The onset purpuric lesions has been referred to occasionally microtraumas on surface, leading vessel rupture for lack an impaired structural collagen support. There evidence that reduction content type I and III dermis, occurs during aging, unable account complexity...