A5034358422- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Pluripotent Stem Cells Research
- Pancreatic and Hepatic Oncology Research
- Immune Cell Function and Interaction
- Genetics and Neurodevelopmental Disorders
- Neuroendocrine Tumor Research Advances
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- Organ Transplantation Techniques and Outcomes
- Phagocytosis and Immune Regulation
- Neuroblastoma Research and Treatments
- Congenital heart defects research
- Endoplasmic Reticulum Stress and Disease
- Renal Transplantation Outcomes and Treatments
- Diabetes Treatment and Management
- Advanced Breast Cancer Therapies
- RNA regulation and disease
- Graphene and Nanomaterials Applications
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2016-2025
Vita-Salute San Raffaele University
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
IRCCS Ospedale San Raffaele
2010-2024
San Raffaele University of Rome
2004-2022
University of Chieti-Pescara
2022
Foundation for Human Potential
2009
Diabetes Australia
2006
The San Raffaele Telethon Institute for Gene Therapy
2005
University of Milano-Bicocca
2005
Objective Type 1 diabetes (T1D) is characterised by islet autoimmunity and beta cell destruction. A gut microbiota–immunological interplay involved in the pathophysiology of T1D. We studied microbiota-mediated effects on disease progression patients with type using faecal microbiota transplantation (FMT). Design Patients recent-onset (<6 weeks) T1D (18–30 years age) were randomised into two groups to receive three autologous or allogenic (healthy donor) FMTs over a period 4 months. Our...
Abstract Purpose: We aimed to assess the safety and efficacy of metformin for treating patients with metastatic pancreatic cancer identify endocrine metabolic phenotypic features or tumor molecular markers associated sensitivity antineoplastic action. Experimental Design: designed an open-label, randomized, phase II trial adding a standard systemic therapy cisplatin, epirubicin, capecitabine, gemcitabine (PEXG) in cancer. Patients ages 18 years older were randomly assigned (1:1) receive PEXG...
Mesenchymal stem cells (MSCs) are the stromal component of bone marrow (BM) and, at moment, most promising prospect for tissue regeneration and repair. MSCs easily obtained from BM, have potential to differentiate into several cell types, show immunomodulatory properties. The use therapies relies on capacity these home engraft long term appropriate target tissue. During past decade, MSC homing BM other organs has been reported. Although mechanisms by which recruited tissues cross endothelial...
Plasticity is a hallmark of macrophages, and in response to environmental signals these cells undergo different forms polarized activation, the extremes which are called classic (M1) alternative (M2). Rapamycin (RAPA) crucial for survival functions myeloid phagocytes, but its effects on macrophage polarization not yet studied. To address this issue, human macrophages obtained from six normal blood donors were M1 or M2 vitro by lipopolysaccharide plus interferon-γ interleukin-4 (IL-4),...
Although long considered a promising treatment option for type 1 diabetes, pancreatic islet cell transformation has been hindered by immune system rejection of engrafted tissue. The identification pathways that regulate post-transplant detrimental inflammatory events would improve management and outcome transplanted patients. Here, we found CXCR1/2 chemokine receptors their ligands are crucial negative determinants survival after transplantation. Pancreatic islets released abundant (CXCL1...
Increasing evidences suggest a correlation between gut and type 1 diabetes (T1D). The objective of this study is to evaluate the inflammatory profile microbiota in patients with T1D compared healthy control (CTRL) subjects celiac disease (CD) as controls. status microbiome composition were evaluated biopsies duodenal mucosa (n = 19), CD CTRL 16) recruited at San Raffaele Scientific Institute, Milan, Italy, 2009 2015. Inflammation was by gene expression immunohistochemistry. Microbiome...
Objectives: Our study aims at producing acellular extracellular matrix scaffolds from the human pancreas (hpaECMs) as a first critical step toward production of new-generation, fully human-derived bioartificial endocrine pancreas. In this pancreas, hardware will be represented by hpaECMs, whereas software consist in cellular compartment generated patient's own cells. Background: Extracellular (ECM)-based obtained through decellularization native organs have become favored platform field...
Long-term clinical outcome of islet transplantation is hampered by the rejection and recurrence autoimmunity. Accurate monitoring may allow for early detection treatment these potentially compromising immune events. Islet transplant was analyzed in 59 consecutive pancreatic recipients whom baseline de novo posttransplant autoantibodies (GAD antibody, insulinoma-associated protein 2 antigen, zinc transporter type 8 antigen) donor-specific alloantibodies (DSA) were quantified. Thirty-nine...
Abstract Dendritic cells (DC) initiate immunity by the activation of naive T and control through their ability to induce unresponsiveness lymphocytes mechanisms that include deletion induction regulatory cells. An inadequate presentation tumor-induced “regulatory” DC, among several mechanisms, can explain tolerance tumor-associated Ags. In this study, we show tumor-derived mucin profoundly affects cytokine repertoire monocyte-derived DC switch them into IL-10highIL-12low APCs with a limited...
The liver is the current site of choice for pancreatic islet transplantation, even though it far from being ideal. We recently have shown in mice that bone marrow (BM) may be a valid alternative to liver, and here we report pilot study test feasibility safety BM as transplantation humans. Four patients who developed diabetes after total pancreatectomy were candidates autologous islet. Because had contraindications intraportal infusion, islets infused BM. In all recipients, engrafted...
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, characterized by studying their secretome before after priming with paclitaxel (PTX). The did not affect the growth cancer cell lines tested vitro, whereas primed (GinPaMSCs/PTX) exerted a significant anticancer effect. able uptake then release amounts pharmacologically effective against cells, as demonstrated...
Over the last few years, human microfragmented adipose tissue (MFAT), containing significant levels of mesenchymal stromal cells (MSCs) and obtained from fat lipoaspirate (LP) through a minimal manipulation in closed system device, has been successfully used aesthetic medicine as well orthopedic general surgery. Interestingly, diseases, this ready-to-use cell derivative seems to have prolonged time efficacy even upon single shot injection into osteoarthritic tissues. Here, we investigated...
Abstract Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts differentiate β from precursors. A thorough characterization these mesenchymal has not yet completed, and there are no conclusive data about their origin. We demonstrated that the outgrowing cultured stem (pMSC) propagate contaminating pMSC. The origin pMSC is partly extrapancreatic both humans mice, by using green fluorescent protein (GFP+) bone...
Abstract Enterovirus 94 (EV‐94) is an enterovirus serotype described recently which, together with EV‐68 and EV‐70, forms human D species. This study investigates the seroprevalences of these three serotypes their abilities to infect, replicate, damage cell types considered be essential for enterovirus‐induced diseases. The studied included leukocyte lines, primary endothelial cells, pancreatic islets. High prevalence neutralizing antibodies against EV‐94 was found in Finnish population....
High levels of donor-derived CCL2 have been associated with poor islet allograft outcome in patients type 1 diabetes. The aim our work was to determine whether secreted by the has independent proinflammatory effects that influence engraftment and graft acceptance. Both mice humans is significantly positively other cytokines/chemokines, particular highly released “proinflammatory” IL-6 CXCL8 or CXCL1. Transplantation CCL2-/- islets into syngenic recipients did not improve transplant function....
Multiple myeloma is an aggressive tumour able to suppress osteoblastogenesis probably mediated by bone marrow mesenchymal stromal cells (BM-MSCs) that can also support plasma cell growth/survival. The use of MSCs for multiple therapy a controversial topic because the contradictory results on capacity inhibit or promote cancer growth. Our previous studies demonstrated could be loaded with Paclitaxel (PTX) and used deliver drug in situ amount affecting growth (in vitro vivo). Therefore,...
Reparixin is an inhibitor of CXCR1/2 chemokine receptor shown to be effective anti-inflammatory adjuvant in a pilot clinical trial allotransplant recipients.A phase 3, multicenter, randomized, double-blind, parallel-assignment study (NCT01817959) was conducted recipients islet allotransplants randomized (2:1) reparixin or placebo addition immunosuppression. Primary outcome the area under curve (AUC) for C-peptide during mixed-meal tolerance test at day 75 ± 5 after first and 365 14 last...
Background. Appropriate recruitment of dendritic cells (DC) at sites inflammation and migration to secondary lymphoid organs is critical importance for the initiation Ag-specific immune responses. The proper localization DC in selected tissues guided primarily by coordinated expression chemokine receptors (CKR). Here we show that immunosuppressive drugs have divergent effects on modulation CKR maturing DC. Methods Results. Dexamethazone (DEX) IL-10 inhibited human CCL19 vitro mouse lymph...