A5033698326- Pancreatic function and diabetes
- Diabetes and associated disorders
- Pancreatic and Hepatic Oncology Research
- Diabetes Management and Research
- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- Neuroendocrine Tumor Research Advances
- Genetics and Neurodevelopmental Disorders
- COVID-19 Clinical Research Studies
- T-cell and B-cell Immunology
- Cannabis and Cannabinoid Research
- Organ Transplantation Techniques and Outcomes
- Pancreatitis Pathology and Treatment
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Adipokines, Inflammation, and Metabolic Diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Phagocytosis and Immune Regulation
- Mesenchymal stem cell research
- Influenza Virus Research Studies
- Tissue Engineering and Regenerative Medicine
- Diabetes Treatment and Management
- Epigenetics and DNA Methylation
- Organ Donation and Transplantation
- Immune cells in cancer
IRCCS Ospedale San Raffaele
2013-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2014-2019
Vita-Salute San Raffaele University
2012-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2019
San Raffaele University of Rome
2003-2013
Diabetes Australia
2006
The San Raffaele Telethon Institute for Gene Therapy
2005
University of Milano-Bicocca
2005
Foundation Center
2004
Plasticity is a hallmark of macrophages, and in response to environmental signals these cells undergo different forms polarized activation, the extremes which are called classic (M1) alternative (M2). Rapamycin (RAPA) crucial for survival functions myeloid phagocytes, but its effects on macrophage polarization not yet studied. To address this issue, human macrophages obtained from six normal blood donors were M1 or M2 vitro by lipopolysaccharide plus interferon-γ interleukin-4 (IL-4),...
Although long considered a promising treatment option for type 1 diabetes, pancreatic islet cell transformation has been hindered by immune system rejection of engrafted tissue. The identification pathways that regulate post-transplant detrimental inflammatory events would improve management and outcome transplanted patients. Here, we found CXCR1/2 chemokine receptors their ligands are crucial negative determinants survival after transplantation. Pancreatic islets released abundant (CXCL1...
Long-term clinical outcome of islet transplantation is hampered by the rejection and recurrence autoimmunity. Accurate monitoring may allow for early detection treatment these potentially compromising immune events. Islet transplant was analyzed in 59 consecutive pancreatic recipients whom baseline de novo posttransplant autoantibodies (GAD antibody, insulinoma-associated protein 2 antigen, zinc transporter type 8 antigen) donor-specific alloantibodies (DSA) were quantified. Thirty-nine...
The aim of the study was to characterise humoral response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with diabetes. Demonstrating ability mount an appropriate antibody presence hyperglycaemia is relevant for comprehension mechanisms related observed worse clinical outcome disease 2019 (COVID-19) pneumonia diabetes and development any future vaccination campaign prevent SARS-CoV-2 infection. Using a highly specific sensitive measurement antibodies by...
Background. Rapamycin is a recently introduced immunosuppressive agent. Its effect on lymphocytes has been extensively studied. Whether it can also modulate dendritic cell (DC) function unknown. Methods. The of rapamycin differentiation, antigen uptake, and the immunostimulatory capacity human DC was examined. were derived from monocytes upon culture with interleukin (IL)-4 granulocyte-macrophage colony-stimulating factor in presence or absence (0.1–100 ng/mL). Surface phenotype uptake...
OBJECTIVE—Leptin and tumor necrosis factor (TNF)-α are associated with insulin resistance cardiovascular disease. In vitro studies suggested that these effects may be mediated via overproduction of monocyte chemoattracting protein (MCP)-1/CCL2, which is a chemokine involved in the pathogenesis atherosclerosis. RESEARCH DESIGN AND METHODS—In this study, fasting plasma leptin, soluble TNF-α receptor 2 (TNF-α-R2), MCP-1/CCL2 concentrations were measured 207 middle-aged women (age 61 ± 12 years,...
Abstract Dendritic cells (DC) initiate immunity by the activation of naive T and control through their ability to induce unresponsiveness lymphocytes mechanisms that include deletion induction regulatory cells. An inadequate presentation tumor-induced “regulatory” DC, among several mechanisms, can explain tolerance tumor-associated Ags. In this study, we show tumor-derived mucin profoundly affects cytokine repertoire monocyte-derived DC switch them into IL-10highIL-12low APCs with a limited...
The liver is the current site of choice for pancreatic islet transplantation, even though it far from being ideal. We recently have shown in mice that bone marrow (BM) may be a valid alternative to liver, and here we report pilot study test feasibility safety BM as transplantation humans. Four patients who developed diabetes after total pancreatectomy were candidates autologous islet. Because had contraindications intraportal infusion, islets infused BM. In all recipients, engrafted...
Monocyte chemoattractant protein-1 (MCP-1/CCL2) is a chemokine involved into the pathogenesis of atherosclerosis and has prognostic value in acute chronic phases patients with coronary syndromes.MCP-1/CCL2 concentration was measured plasma fractions 363 middle-aged overweight/obese individuals (aged 61 +/- 12 years, BMI 30.1 6.6 kg/m(2), 15% type 2 diabetes, 12% impaired glucose tolerance) population survey carried out 1990-1991 Lombardy, Italy (Cremona Study), cardiovascular disease (CVD)...
Chemokines and their receptors have been associated with or implicated in the pathogenesis of type 1 diabetes (T1D), but identification a single specific chemokine/receptor pathway that may constitute suitable target for development therapeutic interventions is still lacking. Here, we used multiple low-dose (MLD) streptozotocin (STZ) injections NOD mouse model to investigate potency CXCR1/2 inhibition prevent inflammation- autoimmunity-mediated damage pancreatic islets. Reparixin ladarixin,...
Abstract Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts differentiate β from precursors. A thorough characterization these mesenchymal has not yet completed, and there are no conclusive data about their origin. We demonstrated that the outgrowing cultured stem (pMSC) propagate contaminating pMSC. The origin pMSC is partly extrapancreatic both humans mice, by using green fluorescent protein (GFP+) bone...
High levels of donor-derived CCL2 have been associated with poor islet allograft outcome in patients type 1 diabetes. The aim our work was to determine whether secreted by the has independent proinflammatory effects that influence engraftment and graft acceptance. Both mice humans is significantly positively other cytokines/chemokines, particular highly released “proinflammatory” IL-6 CXCL8 or CXCL1. Transplantation CCL2-/- islets into syngenic recipients did not improve transplant function....
To assess metabolic and oncologic outcomes of islet autotransplantation (IAT) in patients undergoing pancreatic surgery for either benign or malignant disease.IAT is performed to improve glycemic control after extended pancreatectomy, almost exclusively with chronic pancreatitis. Limited experience available other indications malignancy.In addition pancreatitis, IAT were grade C fistula (treated completion left as indicated); total pancreatectomy an alternative high-risk anastomosis during...
ABSTRACT Influenza A viruses commonly cause pancreatitis in naturally and experimentally infected animals. In this study, we report the results of vivo investigations carried out to establish whether influenza virus infection could metabolic disorders linked pancreatic infection. addition, vitro tests human islets cell lines were performed evaluate viral growth damage. Infection an avian model with two low-pathogenicity isolates caused damage resulting hyperlipasemia over 50% subjects, which...
Islet autotransplantation (IAT) is usually performed in patients undergoing pancreatic surgery for chronic pancreatitis. In the present series, IAT was offered also to both nonmalignant and malignant diseases, having either completion pancreatectomy as treatment severe fistulas (n = 21) or extensive distal neoplasms of neck 19) pancreatoduodenectomy because high risk fistula 32). Fifty-eight 72 who were eligible this broader spectrum indication actually received IAT. There no evidence a...
Reparixin is an inhibitor of CXCR1/2 chemokine receptor shown to be effective anti-inflammatory adjuvant in a pilot clinical trial allotransplant recipients.A phase 3, multicenter, randomized, double-blind, parallel-assignment study (NCT01817959) was conducted recipients islet allotransplants randomized (2:1) reparixin or placebo addition immunosuppression. Primary outcome the area under curve (AUC) for C-peptide during mixed-meal tolerance test at day 75 ± 5 after first and 365 14 last...
To assess whether dysglycemia diagnosed during severe acute respiratory syndrome coronavirus 2 pneumonia may become a potential public health problem after resolution of the infection. In an adult cohort with suspected disease 2019 (COVID-19) pneumonia, we integrated glucose data upon hospital admission fasting blood (FBG) in year prior to COVID-19 and postdischarge follow-up.From February 25 May 15, 2020, 660 adults were admitted San Raffaele Hospital (Milan, Italy). Through structured...
To compare pancreaticoduodenectomy (PD) and total pancreatectomy (TP) with islet autotransplantation (IAT) in patients at high risk of postoperative pancreatic fistula (POPF).
Pancreatogenic diabetes, a consequence of pancreatic tissue loss following pancreatectomy, poses significant challenge for patients undergoing surgery. Islet autotransplantation (IAT) offers promising approach to prevent or alleviate pancreatogenic but its application has been limited individuals with painful chronic pancreatitis.
Background. Appropriate recruitment of dendritic cells (DC) at sites inflammation and migration to secondary lymphoid organs is critical importance for the initiation Ag-specific immune responses. The proper localization DC in selected tissues guided primarily by coordinated expression chemokine receptors (CKR). Here we show that immunosuppressive drugs have divergent effects on modulation CKR maturing DC. Methods Results. Dexamethazone (DEX) IL-10 inhibited human CCL19 vitro mouse lymph...
Islet auto transplantation (IAT) is a potential therapeutic option for patients undergoing pancreatectomy to preserve endocrine function, but its role in with intraductal papillary mucinous neoplasms (IPMN) remains controversial due oncological concerns. This study evaluates the feasibility, safety, and metabolic outcomes of IAT seven focal IPMN who underwent between 2008 2023, following Milan protocol. Primary included technical success islet isolation absence tumor dissemination. Secondary...