A5015698650- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Long-Term Effects of COVID-19
- Diabetes Management and Research
- COVID-19 Clinical Research Studies
- Transplantation: Methods and Outcomes
- Intensive Care Unit Cognitive Disorders
- COVID-19 and Mental Health
- Neurological Complications and Syndromes
- Dialysis and Renal Disease Management
- Nutrition and Health in Aging
- Organ Donation and Transplantation
- Respiratory Support and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Organ and Tissue Transplantation Research
- Neurological and metabolic disorders
- Pancreatitis Pathology and Treatment
- Psychosomatic Disorders and Their Treatments
- Retinal Diseases and Treatments
- Liver Disease and Transplantation
- Pain Mechanisms and Treatments
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
Vita-Salute San Raffaele University
1998-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2020-2024
Istituti di Ricovero e Cura a Carattere Scientifico
1998-2024
IRCCS Ospedale San Raffaele
1990-2024
Weatherford College
2021
San Raffaele University of Rome
1991-2020
The San Raffaele Telethon Institute for Gene Therapy
1998-2002
University of Milan
1991-1998
Mortality of patients with coronavirus disease 2019 (COVID-19), acute respiratory distress syndrome (ARDS), and systemic inflammation is high. In areas pandemic outbreak, the number can exceed maximum capacity intensive care units (ICUs), and, thus, these individuals often receive non-invasive ventilation outside ICU. Effective treatments for this population are needed urgently. Anakinra a recombinant interleukin-1 receptor antagonist that might be beneficial in patient population.We...
The aim of the study was to characterise humoral response against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with diabetes. Demonstrating ability mount an appropriate antibody presence hyperglycaemia is relevant for comprehension mechanisms related observed worse clinical outcome disease 2019 (COVID-19) pneumonia diabetes and development any future vaccination campaign prevent SARS-CoV-2 infection. Using a highly specific sensitive measurement antibodies by...
Background and aim of the work The coronavirus disease-19 (COVID-19) outbreak is posing considerable challenges to healthcare systems societies worldwide. While knowledge on acute phase disease has rapidly expanded, little known consequences COVID-19 following clinical remission. We set up a multidisciplinary follow-up outpatient clinic identify address needs survivors. Here we describe features our programme. Methods assessment comprises complete physical examination, respiratory evaluation...
Coronavirus disease 2019 (COVID-19) may leave behind an altered health status early after recovery. We evaluated the clinical of COVID-19 survivors at three months hospital discharge.In this prospective observational cohort study, hospitalized patients aged ≥18 years, one (M1) and (M3) post-discharge were enrolled. 251 (71.3% males, median [IQR] age 61.8 [53.5-70.7] years) included. Median (IQR) time from discharge to M3 was 89 (79.5-101) days. Primary outcome residual respiratory...
Background UNISCREEN is a general population study aiming at evaluating feasibility and acceptability of universal screening for chronic autoimmune (type 1 diabetes celiac disease) metabolic/cardiovascular diseases (dysglycemia, type 2 diabetes, dyslipidemia, hypertension) across all age groups using capillary blood sampling by fingerprick to measure disease risk markers. Methods was conducted in the Cantalupo fraction, Cerro Maggiore, Milan, Italy, counting 3,061 inhabitants between 100...
Autoimmune destruction of pancreatic beta cells in type I, insulin-dependent diabetes mellitus (IDDM) results the loss endogenous insulin secretion, which is incompletely replaced by exogenous administration. The functional restoration provided allogeneic beta-cell transplantation limited adverse effects immunosuppression. To pursue an replacement therapy based on autologous, engineered human non-beta cells, we generated a retroviral vector encoding genetically modified proinsulin, cleavable...
A motley postacute symptomatology may develop after COVID-19, irrespective of the acute disease severity, age, and comorbidities. Frail individuals have reduced physiological reserves manifested a worse COVID-19 course, during setting. However, it is still unknown, whether frailty subtend some long manifestations. We explored prevalence disturbs in survivals. This was an observational study. Patients aged 65 years or older were followed-up 1, 3, 6 months hospitalization for pneumonia. total...
Background acute illnesses, like COVID-19, can act as a catabolic stimulus on muscles. So far, no study has evaluated muscle mass and quality through limb ultrasound in post-COVID-19 patients. Methods cross sectional observational study, including patients seen one month after hospital discharge for SARS-CoV-2 pneumonia. The underwent multidimensional evaluation. Moreover, we performed dominant medial gastrocnemius (US) to characterize their quality. Results two hundred fifty-nine...
Coronavirus disease 2019 (COVID-19) may lead to neuropsychiatric sequelae. Palmitoylethanolamide (PEA) is an anti-inflammatory and neuroprotective amide used in depressive syndromes. Here we investigate whether micronized/ultramicronized (m/um) PEA improves sequelae COVID-19 survivors. Patients evaluated at our post-COVID-19 outpatient clinic between February August 2021 presenting manifestations ( n = 98) were offered treatment with m/umPEA 600 mg twice daily for 3 months. Those accepting...
OBJECTIVE Recipients of solid organ transplants have an increased risk developing certain types malignancies as compared with the general population. The majority literature has reported on neoplasms in kidney and heart transplant recipients. RESEARCH DESIGN AND METHODS We describe 9 occurring 7 out 73 IDDM patients after simultaneous pancreas transplantation. No cases were recorded among 26 recipients RESULTS Among found 2 posttransplant lymphoproliferative disorder (PTLD), malignant...
Dercum's disease is a rare condition of painful subcutaneous growth adipose tissue. Etiology unknown and pain difficult to control. We report the case 57-year-old man with generalized diffuse disease, who improved after treatment transcutaneous frequency rhythmic electrical modulation system (FREMS). Treatment consisted in 4 cycles 30 minutes FREMS sessions over 6-month period. Measures efficacy included assessment (visual analogue scale, VAS), tissue thickness by magnetic resonance imaging,...
Persistent symptoms including dyspnea and functional impairment are common in COVID-19 survivors. Poor muscle quality (myosteatosis) associates with poor short-term outcomes patients. The aim of this observational study was to assess the relationship between myosteatosis diagnosed during acute patient-reported at 6 months after discharge.Myosteatosis based on CT-derived skeletal radiation attenuation (SM-RA) measured hospitalization 97 survivors who had available anthropometric clinical data...
Abstract Capillary electrophoresis (CE) for the simultaneous and precise quantification of human insulin (hI), proinsulin (hPI) intermediate forms (des 31, 32; split 65–66 des 64, 65), released in culture media by engineered cells, is described. Analytical conditions standard proteins were optimized using a bare silica capillary (20 cm × 50 μm internal diameter). Proteins monitored at 200 nm separated constant voltage. Culture supernatants (12–24 mL) purified on Sep‐Pak Vac C 18 cartridges,...
The aim of this study was to compare the metabolic pathway mature insulin through intermediate forms (32-33 split, 65-66 des31,32 and des64,65) in human or murine cells engineered for release wild-type proinsulin a genetically mutated one, search new approach an insulin-dependent diabetes mellitus cure by gene therapy. Primary fibroblasts, myoblasts stabilized cell lines (HepG2 NIH3T3) were transduced either with retroviral vector coding carrying cleavage sites sensitive furin. pattern all...