A5063579573- Bone Tissue Engineering Materials
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Pluripotent Stem Cells Research
- Microbial Metabolic Engineering and Bioproduction
- Neurogenetic and Muscular Disorders Research
- Dental Implant Techniques and Outcomes
- Gene Regulatory Network Analysis
- Wound Healing and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Bone and Dental Protein Studies
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Adipose Tissue and Metabolism
- Anatomy and Medical Technology
- Magnetic and Electromagnetic Effects
- Orthopaedic implants and arthroplasty
University of Pavia
2015-2024
Technologies pour la Santé
2016-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2001-2009
Sapienza University of Rome
1989-2005
University of Milan
2005
Mario Negri Institute for Pharmacological Research
2005
IFOM
2005
Ospedale Maggiore
2003
Howard Hughes Medical Institute
2003
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2003
Growth and repair of skeletal muscle are normally mediated by the satellite cells that surround fibers. In regenerating muscle, however, number myogenic precursors exceeds resident cells, implying migration or recruitment undifferentiated progenitors from other sources. Transplantation genetically marked bone marrow into immunodeficient mice revealed marrow-derived migrate areas induced degeneration, undergo differentiation, participate in regeneration damaged Genetically modified, could...
Preclinical or clinical trials for muscular dystrophies have met with modest success, mainly because of inefficient delivery viral vectors donor cells to dystrophic muscles. We report here that intra-arterial wild-type mesoangioblasts, a class vessel-associated stem cells, corrects morphologically and functionally the phenotype virtually all downstream muscles in adult immunocompetent alpha-sarcoglycan (alpha-SG) null mice, model organism limb-girdle dystrophy. When mesoangioblasts isolated...
The concept of tissue-restricted differentiation postnatal stem cells has been challenged by recent evidence showing pluripotency for hematopoietic, mesenchymal, and neural cells. Furthermore, rare but well documented examples exist already differentiated in developing mammals that change fate trans-differentiate into another cell type. Here, we report endothelial cells, either freshly isolated from embryonic vessels or established as homogenous culture, differentiate beating cardiomyocytes...
Abstract In this study, we have observed dental pulp stem cells (SBP‐DPSCs) performances on different scaffolds, such as PLGA 85:15, hydroxyapatite chips (HA) and titanium. Stem were challenged with each engineered surface, either in plane cultures or a rotating apparatus, for month. Gingival fibroblasts used controls. Results showed that exerted response, depending the type of textured surface: fact, microconcavities significantly affected SBP‐DPSC differentiation into osteoblasts, both...
Ex vivo gene therapy of primary myopathies, based on autologous transplantation genetically modified myogenic cells, is seriously limited by the number cells that can be isolated, expanded, transduced, and reimplanted into patient's muscles. We explored possibility using MyoD to induce conversion nonmuscle, in a quantitatively relevant fashion. Primary human murine fibroblasts from skin, muscle, or bone marrow were infected an E1-deleted adenoviral vector carrying retroviral long terminal...
ABSTRACT Embryonic and fetal skeletal myoblasts were grown in culture the presence of TGFβ. Under conditions employed, TGFβ inhibited differentiation but not embryonic myoblasts. To investigate possible relevance these data to muscle histogenesis vivo, we studied proliferation/differentiation state mesodermal cells proximal region limb bud at time primary fiber formation. BrdU labeling immunostaining for myosin heavy chains revealed that very few enter S phase cycle when differentiated...
Human mesenchymal stem cells (MSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications. Exposure of MSCs to physical stimuli favors early rapid activation the repair process. In this study we investigated in vitro effects pulsed electromagnetic field (PEMF) treatment on proliferation osteogenic differentiation bone marrow (BM-MSCs) adipose-tissue (ASCs), assess if both types could be indifferently used combination with PEMF exposure healing. We...
Skeletal muscle regeneration is one of the major areas interest in sport medicine as well trauma centers. Three-dimensional (3D) bioprinting (BioP) nowadays widely adopted to manufacture 3D constructs for regenerative but a comparison between available biomaterial-based inks (bioinks) missing. The present study aims assess impact different hydrogels on viability, proliferation, and differentiation murine myoblasts (C2C12) encapsulated bioprinted aided regeneration. We tested three...
The accumulation of two myogenic regulatory proteins, MyoD and myogenin, was investigated by double-immunocytochemistry correlated with myosin heavy chain expression in different classes myoblasts culture during early myogenesis vivo. During vitro differentiation fetal myoblasts, MyoD-positive cells were detected first, followed the appearance positive for both myogenin finally differentiated myocytes myotubes expressing (MHC). A similar pattern observed cultures embryonic satellite cells....
There is increasing interest in designing new biomaterials that could potentially be used the form of scaffolds as bone substitutes. In this study we a hydrophobic crosslinked polyurethane typical tissue-engineering approach, is, seeding and vitro culturing cells using porous scaffold. Using an electromagnetic bioreactor (magnetic field intensity, 2 mT; frequency, 75 Hz), investigated effect stimulation on SAOS-2 human osteoblast proliferation calcified matrix production. Cell was twice...
To test the potential of mesoangioblasts (Mabs) in reducing postischemic injury comparison with bone marrow progenitor cells (BMPCs), fibroblasts (Fbs), and embryonic stem cell-derived endothelial (ECs), to identify putative cellular protective mechanisms.Cells were injected percutaneously left ventricular (LV) chamber C57BL/6 mice, 3 6 hours after coronary ligation, detected hearts 2 days weeks later. Echocardiographic examinations performed at weeks. LV dilation was reduced shortening...
BackgroundScaffold surface features are thought to be important regulators of stem cell performance and endurance in tissue engineering applications, but details about these fundamental aspects biology remain largely unclear.Methodology FindingsIn the present study, smooth clinical-grade lactide-coglyolic acid 85:15 (PLGA) scaffolds were carved as membranes treated with NMP (N-metil-pyrrolidone) create controlled subtractive pits or microcavities. Scanning electron confocal microscopy...
Background Hepatocyte Growth Factor (HGF) is a pleiotropic cytokine of mesenchymal origin that mediates characteristic array biological activities including cell proliferation, survival, motility and morphogenesis. Its high affinity receptor, the tyrosine kinase Met, expressed by wide range tissues can be activated either paracrine or autocrine stimulation. Adult myogenic precursor cells, so called satellite express both HGF Met. Following muscle injury, HGF-Met stimulation plays key role in...
Several studies have shown that low-level laser irradiation (LLLI) has beneficial effects on bone regeneration. The objective of this study was to examine the in vitro LLLI proliferation and differentiation a human osteoblast-like cell line (Saos-2 line). Cultured cells were exposed different doses with semiconductor diode (659 nm; 10 mW power output). assessed daily up seven days culture irradiated once or for three consecutive 1 3 J/cm 2 . obtained results showed stimulation enhances...
Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by decreasing waste polluting potential, due its nutritional load, and producing biofuel renewable source at the same time. Wild type engineered microorganisms have been proposed as biocatalysts. However, they present different drawbacks (e.g., supplements requirement, high...
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are considered a great promise in the repair and regeneration of bone. Considerable efforts have been oriented towards uncovering best strategy to promote osteogenic differentiation. In previous studies, hBM-MSCs exposed physical stimuli such as pulsed electromagnetic fields (PEMFs) or directly seeded on nanostructured titanium surfaces (TiO2) were shown improve their differentiation osteoblasts condition. present study, effect...
Therapy for neurodegenerative lysosomal Tay–Sachs (TS) disease requires active hexosaminidase (Hex) A production in the central nervous system and an efficient therapeutic approach that can act faster than human progression. We combined efficacy of a non-replicating Herpes simplex vector encoding Hex alpha-subunit (HSV-T0alphaHex) anatomic structure brain internal capsule to distribute missing enzyme optimally. With this gene transfer strategy, first time, we re-established activity totally...