A5077140689- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Cardiomyopathy and Myosin Studies
- Cardiac Ischemia and Reperfusion
- Muscle Physiology and Disorders
- Neuroscience and Neural Engineering
- RNA Research and Splicing
- Cardiac electrophysiology and arrhythmias
- Mesenchymal stem cell research
- Cardiac Structural Anomalies and Repair
- Cardiac Imaging and Diagnostics
- Cardiovascular Function and Risk Factors
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Biomedical Ethics and Regulation
- Cardiac Fibrosis and Remodeling
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Cardiac Arrest and Resuscitation
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
University of Washington
2015-2024
Southern California University for Professional Studies
2024
University of Southern California
2024
California Institute for Regenerative Medicine
2008-2023
Sana Biotechnology (United States)
2020-2023
Seattle University
1991-2023
Institute for Stem Cell Biology and Regenerative Medicine
2011-2022
Cardiovascular Research Center
2011-2021
Harvard University
2005-2017
Max Planck Institute for Heart and Lung Research
2017
We have previously shown that a brief episode of ischemia slows the rate ATP depletion during subsequent ischemic episodes. Additionally, intermittent reperfusion may be beneficial to myocardium by washing out catabolites accumulated ischemia. Thus, we proposed multiple episodes might actually protect heart from sustained insult. To test this hypothesis, two sets experiments were performed. In first set, one group dogs (n = 7) was preconditioned with four 5 min circumflex occlusions, each...
Abstract —Vascular calcification is a common finding in atherosclerosis and serious problem diabetic uremic patients. Because of the correlation hyperphosphatemia vascular calcification, ability extracellular inorganic phosphate levels to regulate human aortic smooth muscle cell (HSMC) culture mineralization vitro was examined. HSMCs cultured media containing normal physiological (1.4 mmol/L) did not mineralize. In contrast, comparable those seen hyperphosphatemic individuals (>1.4 showed...
Dilated cardiomyopathy and hypertrophic arise from mutations in many genes. TTN, the gene encoding sarcomere protein titin, has been insufficiently analyzed for because of its enormous size.
We have shown previously that preconditioning myocardium with four 5-minute episodes of ischemia and reperfusion dramatically limited the size infarcts caused by a subsequent 40-minute episode sustained ischemia. The current study was undertaken to assess whether same protocol slowed loss high energy phosphates, catabolite accumulation, and/or delayed ultrastructural damage during ischemic episode. Myocardial metabolites ultrastructure in severely subendocardial regions were compared between...
ABSTRACT Embryonic stem (ES) cells are promising for cardiac repair’ but directing their differentiation toward cardiomyocytes remains challenging. We investigated whether the heart guides ES in vivo and allogeneic were immunologically tolerated. Undifferentiated mouse consistently formed teratomas nude or immunocompetent syngeneic mice. Cardiac contained no more than hind‐limb teratomas’ suggesting lack of guided differentiation. also infarcted hearts’ indicating injury‐related signals did...
Rationale: The developing heart requires both mechanical load and vascularization to reach its proper size, yet the regulation of human growth by these processes is poorly understood. Objective: We seek elucidate responses immature myocardium using tissue engineering approaches. Methods Results: Using embryonic stem cell induced pluripotent cell–derived cardiomyocytes in a 3-dimensional collagen matrix, we show that uniaxial stress conditioning promotes 2-fold increases cardiomyocyte matrix...
We demonstrate here a cardiac tissue-engineering strategy addressing multicellular organization, integration into host myocardium, and directional cues to reconstruct the functional architecture of heart muscle. Microtemplating is used shape poly(2-hydroxyethyl methacrylate-co-methacrylic acid) hydrogel scaffold with architectures driving tissue integration. The construct contains parallel channels organize cardiomyocyte bundles, supported by micrometer-sized, spherical, interconnected pores...
Myocardial infarcts heal by scarring because myocardium cannot regenerate. To determine if skeletal myoblasts could establish new contractile tissue, hearts of adult inbred rats were injured freeze-thaw, and 3-4.5 x 10(6) neonatal muscle cells transplanted immediately thereafter. At 1 d the graft proliferating did not express myosin heavy chain (MHC). By 3 d, multinucleated myotubes present which expressed both embryonic fast fiber MHCs. 2 wk, electron microscopy demonstrated possible...
Understanding pathways controlling cardiac development may offer insights that are useful for stem cell-based repair. Developmental studies indicate the Wnt/β-catenin pathway negatively regulates differentiation, whereas with pluripotent embryonal carcinoma cells suggest this promotes cardiogenesis. This apparent contradiction led us to hypothesize signaling acts biphasically, either promoting or inhibiting cardiogenesis depending on timing. We used inducible promoters activate repress in...
The pervasive expression of circular RNA is a recently discovered feature gene in highly diverged eukaryotes, but the functions most RNAs are still unknown. Computational methods to discover and quantify essential. Moreover, discovering biological contexts where regulated will shed light on potential functional roles they may play.We present new algorithm that increases sensitivity specificity detection by quantifying linear splicing events at both annotated un-annotated exon boundaries,...
Background —Cardiomyocyte grafting augments myocyte numbers in the heart. We investigated (1) how developmental stage influences graft survival; (2) whether acutely necrotic or healing cardiac lesions support grafts; and (3) differentiation integration of cardiomyocyte grafts injured hearts. Methods Results —Cardiomyocytes from fetal, neonatal, adult inbred rats were grafted into normal myocardium, cryoinjured granulation tissue (6 days after injury). Adult cardiomyocytes did not survive...