A5016370550- Nitric Oxide and Endothelin Effects
- Cardiac Ischemia and Reperfusion
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Function and Risk Factors
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Cardiac Arrest and Resuscitation
- Cardiac electrophysiology and arrhythmias
- Cardiac Imaging and Diagnostics
- Cardiac Fibrosis and Remodeling
- Protein Tyrosine Phosphatases
- Cardiovascular Health and Disease Prevention
- Systemic Lupus Erythematosus Research
- Heart Rate Variability and Autonomic Control
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- European and International Law Studies
- Electron Spin Resonance Studies
- Pulmonary Hypertension Research and Treatments
- Anesthesia and Neurotoxicity Research
- Atherosclerosis and Cardiovascular Diseases
- European and International Contract Law
- Apelin-related biomedical research
- Cardiovascular Disease and Adiposity
- Heart Failure Treatment and Management
- Cardiac Valve Diseases and Treatments
Inserm
2015-2024
Université de Rouen Normandie
2015-2024
Centre Hospitalier Universitaire de Rouen
2002-2024
Université Laval
2000-2024
Endothélium, valvulopathies et insuffisance cardiaque
2017-2023
L'Hôpital Nord Franche-Comté
2023
Normandie Université
2013-2022
Université de franche-comté
2016-2022
Centre Hospitalier Universitaire de Besançon
2014-2022
ORCID
2021
Background Experimental evidence suggests that flow-dependent dilatation of conduit arteries is mediated by nitric oxide (NO) and/or prostacyclin. The present study was designed to assess whether NO or prostacyclin also contributes in humans. Methods and Results Radial artery internal diameter (ID) measured continuously 16 healthy volunteers (age, 24±1 years) with a transcutaneous A-mode echo-tracking system coupled Doppler device for the measurement radial blood flow. In 8 subjects,...
We have shown previously that preconditioning myocardium with four 5-minute episodes of ischemia and reperfusion dramatically limited the size infarcts caused by a subsequent 40-minute episode sustained ischemia. The current study was undertaken to assess whether same protocol slowed loss high energy phosphates, catabolite accumulation, and/or delayed ultrastructural damage during ischemic episode. Myocardial metabolites ultrastructure in severely subendocardial regions were compared between...
Endothelin-1 is an endothelium-derived vasoconstrictor peptide. Its circulating levels are below those known to evoke direct vascular effects. To study whether low concentrations of endothelin-1 potentiate the effects other hormones, we suspended isolated human internal mammary and left anterior descending coronary artery rings in organ chambers for isometric tension recording. In rings, contractions norepinephrine (3 x 10(-8) M) were potentiated by threshold 10(-10) (10(-9) (96 +/- 35% 149...
Background— The lymphatic system regulates interstitial tissue fluid balance, and malfunction causes edema. heart has an extensive network displaying a dynamic range of lymph flow in physiology. Myocardial edema occurs many cardiovascular diseases, eg, myocardial infarction (MI) chronic failure, suggesting that cardiac transport may be insufficient pathology. Here, we investigate rats the impact MI subsequent failure on network. Further, evaluate for first time functional effects selective...
Background— Obesity and diabetes mellitus are independently associated with the development of heart failure. In this study, we determined respective effects obesity, insulin resistance, on intrinsic contraction mitochondrial function human myocardium before onset cardiomyopathy. Methods Results— Right atrial was obtained from 141 consecutive patients presenting no sign We investigated ex vivo isometric contraction, respiration calcium retention capacity, respiratory chain complex activities...
Plasma levels of the vasoconstrictor peptide endothelin (ET) are increased in chronic heart failure (CHF), and ET a major predictor mortality this disease. Thus, may play deleterious role CHF. The purpose study was to assess effects treatment with receptor antagonist bosentan rat model CHF.Rats were subjected coronary artery ligation treated for 2 or 9 months placebo (30 100 mg x kg(-1) d(-1)). Bosentan markedly survival (after months: untreated, 47%; bosentan, 65%; P<.01). Throughout...
Heart rate reduction (HRR) improves left ventricular (LV) filling, increases myocardial O2 supply, and reduces consumption, which are all beneficial in congestive heart failure (CHF). However, the long-term effects of HRR on cardiac function remodeling unknown.We assessed, rats with CHF, induced by selective I(f) current inhibitor ivabradine (as food admix for 90 days starting 7 after coronary artery ligation). To assess intrinsic modifications LV tissue HRR, parameters were reassessed 3...
Preconditioning myocardium with brief episodes of ischemia reduces energy demand and delays cell death during a subsequent ischemic episode. We hypothesized that postischemic contractile dysfunction after the ("stunning") causes this reduced demand. If hypothesis is correct, then cardioprotection should persist as long mechanical function still depressed at onset sustained ischemia. To analyze temporal relationship between preconditioning stunning, infarct size was compared in two groups...
Repetitive, brief periods of ischemia and reperfusion ("preconditioning") increase the resistance myocardial tissue to subsequent prolonged ischemic episodes limit infarct size. We investigated whether preconditioning also protects against coronary endothelial dysfunction induced by reperfusion.Experiments were performed in four groups rats (n = 8 each group): group 1 underwent sham surgery, 2 subjected 20 minutes left artery occlusion without reperfusion, 3 followed hour 4 (preconditioning...
Toll-like receptors (TLR) 2 are expressed in cardiac and inflammatory cells, regulate leukocyte function. Because adhesion is a critical event endothelial injury induced by ischemia/reperfusion (I/R), we assessed whether TLR2 were involved I/R-induced coronary injury.Ischemia-reperfusion markedly decreased NO-mediated relaxations to acetylcholine ex vivo. In contrast, deficient mice, I/R paradoxically improved the responses acetylcholine. To precise cellular compartment expressing which...
Microparticles (MPs) are small fragments generated from the plasma membrane after cell stimulation.Among candidate proteins harbored by MPs, we recently showed that sonic hedgehog (Shh) is present in MPs activated/apoptotic human T lymphocytes [Martı ´nez et al., Blood (2006) vol.108, 3012-3020].We show here Shh carried induces nitric oxide (NO) release endothelial cells, triggers changes expression and phosphorylation of enzymes related to NO pathway, decreases production reactive oxygen...
Cytochrome-derived epoxyeicosatrienoic acids may be important endothelium-derived hyperpolarizing factors, opening calcium-activated potassium channels, but their involvement in the regulation of arterial stiffness during changes blood flow humans is unknown. In healthy volunteers, we measured pressure, radial artery diameter, wall thickness, and (NIUS02) hand skin heating presence saline or inhibitors NO synthase (N(G)-monomethyl-L-arginine), channels (tetraethylammonium), cytochrome...
Lymphatics play an essential pathophysiological role in promoting fluid and immune cell tissue clearance. Conversely, cells may influence lymphatic function remodeling. Recently, cardiac lymphangiogenesis has been proposed as a therapeutic target to prevent heart failure after myocardial infarction (MI). We investigated the effects of gene therapy modulate post-MI rodents. Second, we determined impact cardiac-infiltrating T on remodeling heart. Approach Results: Comparing adenoviral versus...
We report here a unique example of an in situ generated aluminum initiator stabilized by C2-symmetric salen ligand which shows hitherto unknown high activity for the ROP rac-lactide at room temperature. Using simple and robust catalyst system, is prepared from complex onium salt, this convenient route employs readily available reagents that afford polylactide good yields with narrow polydispersity indices, without need time-consuming expensive processes are typically required preparation...
Continuous production of nitric oxide (NO) from endothelial cells permanently inhibits the synthesis and vasoconstrictor effects endothelin. Thus, inhibition NO might unmask a vasopressor response to To assess whether endothelin contributes pressor induced by synthesis, we tested bosentan, nonpeptide antagonist ETA ETB receptors, affected hypertensive synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME).Anesthetized rats received increasing doses L-NAME (0.1 3 mg.kg-1) in absence or...
BACKGROUND-Chronic heart failure (CHF) impairs the endothelium-dependent, flow-mediated dilation (FMD) of small arteries. However, whether chronic angiotensin-converting enzyme (ACE) inhibition affects impairment FMD in CHF is unknown. We investigated effects long-term ACE on peripheral arteries rats with and mechanism(s) involved. METHODS AND RESULTS-FMD was assessed isolated, perfused gracilis muscle from sham-operated, untreated or inhibitor-treated (perindopril 2 mg. kg(-1). day(-1) for...
The myocardial velocity gradient (MVG) is a recent index of regional function derived from endocardial and epicardial velocities obtained by tissue Doppler imaging (TDI). This might be useful for discriminating between physiological pathological left ventricular hypertrophy (LVH) documenting the early transition compensated LVH to heart failure. We sought compare MVG measured across posterior wall normal rats with (exercise) (pressure-overload) LVH.Wistar were assigned one following groups:...
Flow-mediated dilatation (FMD) of the peripheral arteries may be impaired in chronic heart failure (CHF), and this could contribute to increased resistance exercise intolerance that occur with disease. Physical improves FMD large conduit CHF, but whether a similar impairment also occurs smaller is unknown. The mechanisms changes after CHF or are unknown.FMD was assessed isolated, perfused, preconstricted gracilis muscle from sham-operated rats (coronary artery ligation) who were either...
Recent studies suggest that polymorphonuclear leukocytes (PMNs) may cause additional myocyte injury during reperfusion of ischemic myocardium. The present study was done to investigate whether PMNs accumulate in myocardium early after reversible or irreversible injury. Open-chest anesthetized dogs underwent circumflex coronary occlusions for 12 min (n = 5), 40 8), 90 followed by 1 h reperfusion. Autologous were radiolabeled with 111In and reinjected quantitate myocardial PMN influx reflow....