A5018614739- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Pancreatic function and diabetes
- Congenital heart defects research
- Diabetes and associated disorders
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Nitric Oxide and Endothelin Effects
- Adipose Tissue and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Mesenchymal stem cell research
- Viral Infections and Immunology Research
- Adipokines, Inflammation, and Metabolic Diseases
- Signaling Pathways in Disease
- Virus-based gene therapy research
- RNA regulation and disease
- Peroxisome Proliferator-Activated Receptors
Humanitas University
2012-2019
University of Naples Federico II
1991-2018
IRCCS Humanitas Research Hospital
2012-2016
University of Palermo
2002-2016
The Ohio State University
2016
University of Debrecen
2016
University of KwaZulu-Natal
2016
Fondazione Humanitas per la Ricerca
1992-2014
University of Milan
2013
MultiMedica
2007-2012
Cardiac myocytes have been traditionally regarded as terminally differentiated cells that adapt to increased work and compensate for disease exclusively through hypertrophy. However, in the past few years, compelling evidence has accumulated suggesting heart regenerative potential. Recent studies even surmised existence of resident cardiac stem cells, endothelial generating cardiomyocytes by cell contact or extracardiac progenitors cardiomyocytes, but these findings are still controversial....
The serine-threonine kinase Akt seems to be central in mediating stimuli from different classes of receptors. In fact, both IGF-1 and IL6-like cytokines induce hypertrophic antiapoptotic signals cardiomyocytes through PI3K-dependent activation. More recently, it was shown that is involved also the effects β-adrenergic stimulation. Thus, determine on cardiac function vivo , we generated a model cardiac-specific overexpression mice. Transgenic mice were by using E40K, constitutively active...
Mechanistic target of rapamycin (MTOR) plays a critical role in the regulation cell growth and response to energy state changes. Drugs inhibiting MTOR are increasingly used antineoplastic therapies. Myocardial activity changes during hypertrophy heart failure (HF). However, whether exerts positive or negative effect on myocardial function remains be fully elucidated. Here, we show that ablation Mtor adult mouse myocardium results fatal, dilated cardiomyopathy is characterized by apoptosis,...
Cardiac hypertrophy, initially an adaptive response of the myocardium to stress, can progress heart failure. The epigenetic signature underlying this phenomenon is poorly understood. Here, we report on genome-wide distribution seven histone modifications in adult mouse cardiomyocytes subjected a prohypertrophy stimulus vivo. We found set promoters with pattern that distinguishes specific functional classes genes regulated hypertrophy and identified 9,207 candidate active enhancers whose...
The concept of tissue-restricted differentiation postnatal stem cells has been challenged by recent evidence showing pluripotency for hematopoietic, mesenchymal, and neural cells. Furthermore, rare but well documented examples exist already differentiated in developing mammals that change fate trans-differentiate into another cell type. Here, we report endothelial cells, either freshly isolated from embryonic vessels or established as homogenous culture, differentiate beating cardiomyocytes...
AimsRecent studies have demonstrated the importance of chromatin remodelling via histone acetylation/deacetylation for control cardiac gene expression. Specific deacetylases (HDACs) can, in fact, play a positive or negative role determining myocyte (CM) size. Here, we report on effect hypertrophy development three inhibitors (HDACi) class I HDACs.
Abstract Methylation at 5-cytosine (5-mC) is a fundamental epigenetic DNA modification associated recently with cardiac disease. In contrast, the role of 5-hydroxymethylcytosine (5-hmC)—5-mC’s oxidation product—in biology and disease unknown. Here we assess hydroxymethylome in embryonic, neonatal, adult hypertrophic mouse cardiomyocytes, showing that dynamic modulation hydroxymethylated specific transcriptional networks during heart development failure. hydroxymethylation marks body highly...
Skeletal muscle tissue engineering is a promising approach for the treatment of muscular disorders. However, complex organization muscle, combined with difficulty in finding an appropriate source regenerative cells and providing adequate blood supply to engineered tissue, makes this hard task face. In present work, we describe innovative rejuvenate adult skeletal muscle-derived pericytes (MP) based on use PEG-based hydrogel scaffold. MP were isolated from young (piglet) (boar) pigs assess...
Cell-based regenerative therapies are significantly improved by engineering allografts to express factors that increase vascularization and engraftment, such as placental growth factor (PlGF) matrix metalloproteinase 9 (MMP9). Moreover, the seeding of therapeutic cells onto a suitable scaffold is utmost importance for tissue regeneration. On these premises, we sought assess reparative potential induced pluripotent stem (iPS) bioengineered secrete PlGF or MMP9 delivered infarcted myocardium...
Cardiomyocytes adapt to physical stress by increasing their size while maintaining cell function. The serine/threonine kinase Akt plays a critical role in this process of adaptation. We previously reported that transgenic overexpression an active form (Akt-E40K) mice results increased cardiac contractility and size, as well improved sarcoplasmic reticulum (SR) Ca2+ handling. Because it is not fully elucidated, we decided study the molecular mechanism which Akt-E40K improves SR To end, uptake...
Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of few embryonic transcription factors. The resulting induced stem (iPS) differentiate into all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack capacity proliferate. Here, we report neonatal CMs generate iPS express embryonic-specific markers and feature gene-expression profiles similar those mouse (mES) cell cardiac fibroblast (CF)-derived populations. CM-derived are able...