Glycerol outflow from adipocytes has been considered for a decade to be mediated by aquaporin-7, an aquaglyceroporin highly expressed in the adipose tissue. Its involvement glycerol metabolism widely studied also humans. Recent studies different aquaporin-7 KO mice models pose two questions 1) exact localization of human white tissue; 2) existence other aquaglyceroporins that work with guarantee efflux and thus normal adiposity To this purpose we investigated expression, functioning...

Human mesenchymal stem cells (MSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications. Exposure of MSCs to physical stimuli favors early rapid activation the repair process. In this study we investigated in vitro effects pulsed electromagnetic field (PEMF) treatment on proliferation osteogenic differentiation bone marrow (BM-MSCs) adipose-tissue (ASCs), assess if both types could be indifferently used combination with PEMF exposure healing. We...

Different aquaporins (AQPs) are expressed in human sperm cells and with a different localization. Their function has been related to cell volume control response the osmotic changes encountered passing from epididymal fluid cervical mucus or involved end stage of cytoplasm removal during maturation. Recently, AQPs have also shown hydrogen peroxide (H₂O₂) permeability properties. Here, we investigate expression, localization functioning particular attention their role as peroxiporins reactive...

The use of stem cells in regenerative medicine is an appealing area research that has received a great deal interest recent years. population called human adipose tissue-derived (hASCs) share many the characteristic its counterpart marrow including extensive proliferative potential and ability to undergo multilineage differentiation along classical mesenchymal lineages: adipogenesis, chondrogenesis, osteogenesis, myogenesis. aim this study was evaluate with biochemical morphological methods...

Background information . In the gastrointestinal tract of mammals, water can either be secreted with digestive juices or absorbed by small and large intestine. Transcellular movement mediated transmembrane protein family AQPs (aquaporins), as has also been recently identified in tract. However, localization, expression functioning have not completely characterized. For present study, we investigated: (1) AQP7 some portions rat semiquantitative reverse transcriptase—PCR immunoblotting (2)...

Journal Article EFFECTS OF ACUTE AND CHRONIC ETHANOL ADMINISTRATION ON THIAMINE METABOLIZING ENZYMES IN SOME BRAIN AREAS OTHER ORGANS THE RAT Get access U. LAFORENZA, LAFORENZA Institute of Human Physiology, University PaviaPavia, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar C. PATRINI, PATRINI G. GASTALDI, GASTALDI RINDI * To whom correspondence should be addressed. Alcohol and Alcoholism, Volume 25, Issue 6, 1990, Pages 591–603,...

Celiac disease is a chronic inflammatory disorder of the small bowel induced in genetically susceptible subjects by gluten ingestion. Diarrhoea, weight loss and malabsorption represent major clinical presentation disease. Here we examined possible alteration expression localization water channels [AQPs (aquaporins)] some solute transporters duodenal mucosa celiac patients. Duodenal biopsies from untreated celiacs, treated healthy controls were considered present study. The expressions AQPs...

Pluripotent adipose tissue-derived stem cells (hASCs) can differentiate into various mesodermal cell types such as osteoblasts, chondroblasts, and myoblasts. We isolated hASCs from subcutaneous tissue during orthopaedic surgery induced the osteogenic differentiation for 28 days on three different synthetic scaffolds polylactide-co-glycolide (PLGA), polylactide-co-glycolide/hydroxyapatite (PLGA/HA), trabecular titanium (Ti6Al4V). Pore size influence certain criteria attachment, infiltration,...

Trabecular Titanium (TT) is an innovative highly porous structure that imitates the morphology of trabecular bone with good mechanical properties. Adipose-derived stem cells are a multipotent cell population can be used in regenerative medicine, particular, for therapeutic applications. The ability TT to induce osteogenic differentiation human adipose derived (hASCs) absence factors was evaluated using molecular biological, biochemical, and immunohistochemical methods. At 7 21 days from...

Some aquaporins (AQPs) have been recently demonstrated to facilitate the diffusion of hydrogen peroxide (H2O2) from producing cells extracellular fluid, and their reactive oxygen species scavenging properties defined. Nevertheless, identification different AQPs acting as peroxiporins, functional role in eustress distress, antioxidant compounds able regulate AQP gating, remain unsolved. This study aims investigate, HeLa cells: (1) expression AQPs; (2) evaluation naringenin, quercetin,...

Adipose tissue comprises both adipose and non-adipose cells such as mesenchymal stem cells. These show a surface antigenic profile similar to that of bone-marrow-derived MSC. The derived from the dedifferentiation mature adipocytes (DFAT) are another cell population with characteristics stemness. aim this study is provide evidence stemness, proliferation, differentiation human (hASC) DFAT obtained subcutaneous AT evaluate their potential use in regenerative medicine. Cell populations were...

Human sperm cells express different aquaporins (AQPs), AQP3, 7, 8, 11, which are localized both in the plasma membrane and intracellular structures. Besides cell volume regulation end stage of cytoplasm removal during maturation, role AQPs extends also to reactive oxygen species (ROS) elimination. Moreover, oxidative stress has been shown inhibit AQP-mediated H2O2 permeability. A decrease functionality is related a number motility. Here we investigate possible effect human Papillomavirus...

1. Microvillous vesicles obtained by a Ca2+ precipitation method from the intestine of adult Wistar albino rats were incubated at 25 degrees C with [35S]‐ or [3H]thiamine high specific activity. 2. The time course thiamine uptake was not influenced presence Na+ K+ nor absence alkaline cations in incubation medium. 3. At concentrations below 1.25 microM, taken up mainly saturable mechanism apparent Km = 0.8 microM and Vmax 0.35 pmol mg protein‐1 4 s‐1. higher concentrations, non‐saturable...

The effect of long-term ethanol administration on the membrane mechanism thiamine entry in rat enterocytes was investigated by using microvillous vesicles small intestine. Experiments were carried out three groups Wistar albino rats both sexes (290–400 g initial body wt). Group I did not receive any treatment, group II received 4.7 ethanol/kg wt (as a 50% hydroalcoholic solution) daily gastric gavage for 35 days and III (pair-fed controls) solution saccharose (isoenergetic with dose...

Several aquaporins (a family of integral membrane proteins) have been recently identified in the mammalian gastrointestinal tract, and their involvement movement fluid small solutes has suggested. In this direction we investigated, some regions rat presence localization aquaporin-6, given its peculiar function as an ion selective channel. RT-PCR immunoblotting experiments showed that aquaporin-6 was expressed all investigated portions tract. The transcript highly intestine rectum, less...

Water channels AQP7 and AQP8 may be involved in transcellular water movement the small intestine. We show that both mRNA are expressed rat Immunoblot immunohistochemistry experiments demonstrate proteins present apical brush border membrane of intestinal epithelial cells. investigated effect several metals pH on osmotic permeability (Pf) vesicles (BBMVs) a cell line. Hg2+, Cu2+, Zn2+ caused significant decrease BBMV Pf, whereas Ni2+ Li+ had no effect. AQP8-transfected cells showed reduction...

The objective of this study was to investigate the surface modification a natural bone substitute, Bio-Oss, coated with synthetic polymer poly-D,L-lactide (PLA), in order improve cell growth. Bio-Oss is substitute made m

Abstract Spine degenerative conditions are becoming increasingly prevalent, affecting about 5.7% of the population in Europe, resulting a significant reduction life’s quality. Up to now, many materials have been used manufacturing cage implants, as graft substitutes, achieve immediate and long-term spinal fixation. Particularly, titanium its alloys emerging valuable candidates develop new types cages. The aim this vitro study was evaluate adhesion, proliferation osteogenic differentiation...