A5056725317- Bone health and osteoporosis research
- Bone health and treatments
- Bone Metabolism and Diseases
- Growth Hormone and Insulin-like Growth Factors
- Parathyroid Disorders and Treatments
- Ion channel regulation and function
- Vitamin D Research Studies
- Metabolism and Genetic Disorders
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Estrogen and related hormone effects
- Magnesium in Health and Disease
- Neuropeptides and Animal Physiology
- Bone and Joint Diseases
- Mitochondrial Function and Pathology
- Regulation of Appetite and Obesity
- Renal function and acid-base balance
- Muscle activation and electromyography studies
- Planarian Biology and Electrostimulation
- Kidney Stones and Urolithiasis Treatments
- Lipid Membrane Structure and Behavior
- Orthopaedic implants and arthroplasty
- Protein Hydrolysis and Bioactive Peptides
- Body Composition Measurement Techniques
- HIV-related health complications and treatments
IRCCS Ospedale San Raffaele
2002-2025
Vita-Salute San Raffaele University
2013-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2003-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2003-2023
San Raffaele University of Rome
2003-2019
United States Bone and Joint Initiative
1999-2010
University of Milan
1984-1992
University of Pennsylvania
1988
KU Leuven
1986
Bisphosphonates are effective agents for the management of osteoporosis. Their low bioavailability and potency necessitate frequent administration on an empty stomach, which may reduce compliance. Gastrointestinal intolerance limits maximal dosing. Although intermittent intravenous treatments have been used, optimal doses dosing interval not systematically explored.
Growing evidence suggests that vitamin D deficiency may be one of the most important environmental factors for development multiple sclerosis (MS).The objectives this paper are to evaluate serum 25-hydroxyvitamin (25(OH)D) levels in patients with clinically isolated syndromes (CIS) and examine whether they related MS risk.This is a retrospective study 100 CIS hospitalized from 2000 2009 at San Raffaele Hospital, Milan, Italy. We evaluated baseline 25(OH)D level as well clinical, brain...
Transgenic mice over-expressing calcitonin gene-related peptide (CGRP) in osteoblasts have increased bone density due to formation, thus suggesting that CGRP plays a role metabolism. In this study we determined the relationship between CGRP, canonical Wnt signaling and apoptosis human (hOBs) consideration of well-documented involvement pathway cells. Primary cultures hOBs were treated with 10(-8) M. Levels β-catenin, which is cytoplasmic protein mediator signaling, mRNA determined. increases...
The calcitonin peptides [calcitonin (CT), gene-related peptide (CGRP), amylin] share many biological actions, including activity on bone cells. In the present study, CT (10 −11 to 10 −9 M) stimulated [ 3 H]thymidine incorporation in primary cultures of human osteoblasts (hOB), as already demonstrated for CGRP and amylin. RT-PCR analysis showed that receptor receptor-like are both expressed hOB. these cells, −10 amylin M), contrast −8 did not increase cAMP production. All three protein kinase...
To evaluate the potential role of quantitative ultrasound (QUS) investigation in assessing osteopenia prematurity.QUS parameters measured at time discharge were related to anthropometric characteristics and age (postnatal gestational) 51 (34 female 17 male) preterm infants fed fortified human milk.QUS evaluation was performed humerus (h) by measuring two parameters: velocity (hSOS, m/s) bone transmission (hBTT, micros). A group 43 term (29 14 also evaluated.In infants, significant...
The uPAR and its ligand uPA are expressed by both osteoblasts osteoclasts. Their function in bone remodeling is unknown. We report that uPAR-lacking mice display increased BMD, osteogenic potential of osteoblasts, decreased osteoclasts formation, altered cytoskeletal reorganization mature osteoclasts.Urokinase receptor (uPAR) actively involved the regulation important cell functions, such as proliferation, adhesion, migration. It was previously shown major players remodeling, osteoclasts,...
Bone fragility and associated fracture risk are major problems in aging. Oxidative stress mitochondrial dysfunction play a key role the development of bone fragility. Mitochondrial is closely with excessive production reactive oxygen species (ROS). L-Carnitine (L-C), fundamental cofactor lipid metabolism, has an important antioxidant property. Several studies have shown how L-C enhances osteoblastic proliferation activity. In current study, we investigated potential effects on activity, ROS...
Trabecular titanium implants are widely used in orthopedic surgery and known to promote osseointegration. In this study, we investigated whether primary human osteoblast-like cells grown inside a 3D trabecular scaffold undergo changes migration capacity, transcriptomic profile, cellular phenotype as compared the same osteoblasts not scaffold. Scratch tests have shown that of from external environment into Next generation sequencing analysis demonstrated modified expression genes involved...
The osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL)/receptor (RANK) system was evaluated as a potential target CGRP anabolic activity on bone. Primary cultures human osteoblast-like cells (hOB) express calcitonin receptor-like receptor (CLR) and modifying protein 1, and, because stimulates cAMP (one the modulators OPG production in osteoblasts), it investigated whether affects secretion expression hOB. treatment hOB (10 −11 M–10 −7 M) dose-dependently inhibited...
To characterize osteoprotegerin (OPG) levels, bone remodelling and mineral density (BMD) in heavily pretreated HIV-infected patients on antiretroviral therapy, to evaluate the clinical factors associated with decline.Heavily (> 5 years) HIV-positive were enrolled this cross-sectional, observational study, which was based a total body densitometry examination comprehensive evaluation of parameters.Sixty-eight (55 male 13 female) median age 41 years (range 25-60 included study. Their treatment...
Hypophosphatasia (HPP) is a rare autosomal dominant or recessive metabolic disorder caused by mutations in the tissue nonspecific alkaline phosphatase gene (ALPL). To date, over 300 different ALPL have been identified. Disease severity widely variable with severe forms usually manifesting during perinatal and/or infantile periods while mild are sometimes only diagnosed adulthood remain undiagnosed. Common clinical features of HPP defects bone and tooth mineralization along biochemical...
While multiple myeloma (MM) is almost invariably preceded by asymptomatic monoclonal gammopathy of undetermined significance (MGUS) and/or smoldering MM (SMM), the alterations bone marrow (BM) microenvironment that establish progression to symptomatic disease are circumstantial. Here we show in Vk*MYC mice harboring oncogene-driven plasma cell proliferative disorder, appearance associated with substantial modifications BM microenvironment, including a progressive accumulation both CD8+ and...
It is presently thought that osteoprotegerin (OPG) a cytokine involved in the regulation of osteoblast/osteoclast crosstalk and maintenance bone mass. Recent studies showed GH replacement therapy GH-deficient patients was able to induce significant increase OPG plasma, as well cortical trabecular bone. In order determine whether could directly modulate secretion, effect on human osteoblast-like cells (hOB) primary culture studied. After detecting presence mRNA for receptor (GHR) by RT-PCR,...
Isolated populations are a useful resource for mapping complex traits due to shared stable environment, reduced genetic complexity and extended Linkage Disequilibrium (LD) compared the general population. Here we describe large isolate from North West Apennines, mountain range that runs through Italy Alps South.The study involved 1,803 people living in 7 villages of upper Borbera Valley. For this population cohort, data genealogy reconstruction, medical questionnaires, blood, anthropometric...