A5045980519- Bone Metabolism and Diseases
- Growth Hormone and Insulin-like Growth Factors
- Neuropeptides and Animal Physiology
- Bone health and osteoporosis research
- Receptor Mechanisms and Signaling
- Bone health and treatments
- Mast cells and histamine
- Metabolism and Genetic Disorders
- Hemoglobinopathies and Related Disorders
- Protein Hydrolysis and Bioactive Peptides
- Neuroendocrine regulation and behavior
- Planarian Biology and Electrostimulation
- Estrogen and related hormone effects
- Erythrocyte Function and Pathophysiology
- Organ Transplantation Techniques and Outcomes
- Multiple Myeloma Research and Treatments
- Circadian rhythm and melatonin
- Mesenchymal stem cell research
- GDF15 and Related Biomarkers
- Birth, Development, and Health
- Advanced X-ray and CT Imaging
- Regulation of Appetite and Obesity
- Wnt/β-catenin signaling in development and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- TGF-β signaling in diseases
Vita-Salute San Raffaele University
2013-2024
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2023
IRCCS Ospedale San Raffaele
1988-2022
San Raffaele University of Rome
2000-2014
University of Milan
1986-2006
United States Bone and Joint Initiative
1999
University of Pavia
1994
The Neurological Institute
1994
Transgenic mice over-expressing calcitonin gene-related peptide (CGRP) in osteoblasts have increased bone density due to formation, thus suggesting that CGRP plays a role metabolism. In this study we determined the relationship between CGRP, canonical Wnt signaling and apoptosis human (hOBs) consideration of well-documented involvement pathway cells. Primary cultures hOBs were treated with 10(-8) M. Levels β-catenin, which is cytoplasmic protein mediator signaling, mRNA determined. increases...
The molecular basis underlying the clinical phenotype in bone diseases is customarily associated with abnormal extracellular matrix structure and/or properties. More recently, cellular malfunction has been identified as a concomitant causative factor and increased attention focused on stem cells differentiation. Classic osteogenesis imperfecta (OI) prototype for heritable dysplasias: it dominant genetic transmission caused by mutations genes coding collagen I, most abundant protein bone....
The calcitonin peptides [calcitonin (CT), gene-related peptide (CGRP), amylin] share many biological actions, including activity on bone cells. In the present study, CT (10 −11 to 10 −9 M) stimulated [ 3 H]thymidine incorporation in primary cultures of human osteoblasts (hOB), as already demonstrated for CGRP and amylin. RT-PCR analysis showed that receptor receptor-like are both expressed hOB. these cells, −10 amylin M), contrast −8 did not increase cAMP production. All three protein kinase...
Bone fragility and associated fracture risk are major problems in aging. Oxidative stress mitochondrial dysfunction play a key role the development of bone fragility. Mitochondrial is closely with excessive production reactive oxygen species (ROS). L-Carnitine (L-C), fundamental cofactor lipid metabolism, has an important antioxidant property. Several studies have shown how L-C enhances osteoblastic proliferation activity. In current study, we investigated potential effects on activity, ROS...
The osteoprotegerin (OPG)/receptor activator of nuclear factor-κB ligand (RANKL)/receptor (RANK) system was evaluated as a potential target CGRP anabolic activity on bone. Primary cultures human osteoblast-like cells (hOB) express calcitonin receptor-like receptor (CLR) and modifying protein 1, and, because stimulates cAMP (one the modulators OPG production in osteoblasts), it investigated whether affects secretion expression hOB. treatment hOB (10 −11 M–10 −7 M) dose-dependently inhibited...
While multiple myeloma (MM) is almost invariably preceded by asymptomatic monoclonal gammopathy of undetermined significance (MGUS) and/or smoldering MM (SMM), the alterations bone marrow (BM) microenvironment that establish progression to symptomatic disease are circumstantial. Here we show in Vk*MYC mice harboring oncogene-driven plasma cell proliferative disorder, appearance associated with substantial modifications BM microenvironment, including a progressive accumulation both CD8+ and...
It is presently thought that osteoprotegerin (OPG) a cytokine involved in the regulation of osteoblast/osteoclast crosstalk and maintenance bone mass. Recent studies showed GH replacement therapy GH-deficient patients was able to induce significant increase OPG plasma, as well cortical trabecular bone. In order determine whether could directly modulate secretion, effect on human osteoblast-like cells (hOB) primary culture studied. After detecting presence mRNA for receptor (GHR) by RT-PCR,...
Though extensive studies have been conducted, questions regarding the molecular effectors and pathways underlying regulatory role of 1,25(OH)(2)D(3) in human osteoblasts other than cell differentiation matrix protein production remain unanswered. This study aims to identify genes that are modulated by treatment osteoblasts. Primary osteoblast cultures obtained from bone tissue samples were treated with (10(-7) M) for 24 h their transcritptomes profiled microarray analysis using Affymetrix...
Abstract The present investigation was carried out to analyse, immunohistochemically, in vivo leptin expression cartilage and bone cells, the latter restricted elements of osteogenic system (stromal osteoblasts, osteocytes, lining cells). Observations were performed on first lumbar vertebra, tibia femur four rats humerus, acromion patients. Histological sections paraffin‐embedded samples immunostained using antibody leptin. results showed that, growing rat bone, is expressed chondrocytes...
Betaine (BET), a component of many foods, is an essential osmolyte and source methyl groups; it also shows antioxidant activity. Moreover, BET stimulates muscle differentiation via insulin like growth factor I (IGF-I). The processes myogenesis osteogenesis involve common mechanisms with skeletal cells osteoblasts sharing the same precursor. Therefore, we have hypothesized that might be effective on osteoblast cell differentiation. effect was tested in human (hObs) derived from trabecular...
Clinical evidence highlights a relationship between the blood and bone, but underlying mechanism linking these two tissues is not fully elucidated. Here, we used β-thalassemia as model of congenital anemia with bone marrow (BM) niche defects. We demonstrate that fibroblast growth factor 23 (FGF23) increased in patients mice because erythropoietin induces FGF23 overproduction BM erythroid cells via ERK1/2 STAT5 pathways. show vivo inhibition signaling by carboxyl-terminal peptide safe...