A5073327110- Gastric Cancer Management and Outcomes
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Metastasis and carcinoma case studies
- MRI in cancer diagnosis
- Cancer Cells and Metastasis
- Prostate Cancer Diagnosis and Treatment
- Radiomics and Machine Learning in Medical Imaging
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- CAR-T cell therapy research
- Colorectal Cancer Surgical Treatments
- Colorectal Cancer Screening and Detection
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
- Gastrointestinal Tumor Research and Treatment
- Protease and Inhibitor Mechanisms
- Esophageal Cancer Research and Treatment
- Tuberculosis Research and Epidemiology
- Helicobacter pylori-related gastroenterology studies
- Genetic Neurodegenerative Diseases
- T-cell and B-cell Immunology
Vita-Salute San Raffaele University
2015-2024
University of Milan
2024
Centro di Ricerca per la Patologia Vegetale
2024
San Raffaele University of Rome
2006-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2009-2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2023
University Foundation
2022
IRCCS Ospedale San Raffaele
2020
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2016
The Technological College of Beer Sheva
2016
γ-Retroviral vectors (γRVs), which are commonly used in gene therapy, can trigger oncogenesis by insertional mutagenesis. Here, we have dissected the contribution of vector design and viral integration site selection (ISS) to using an vivo genotoxicity assay based on transplantation vector-transduced tumor-prone mouse hematopoietic stem/progenitor cells. By swapping genetic elements between γRV lentiviral (LVs), demonstrated that transcriptionally active long terminal repeats (LTRs) major...
In Brief Purpose: To investigate whether the ratio between metastatic and examined lymph nodes (N ratio) is a better prognostic factor as compared with traditional staging systems in patients gastric cancer regardless of extension node dissection. Patients & Methods: We retrospectively reviewed data 1853 who underwent radical resection for carcinoma at 6 Italian centers. >15 (group 1, n = 1421) those ≤15 2, 432) were separately analyzed. N categories 0, 0%; 1%–9%; 10%–25%; 3, >25%)...
Abstract The potential virucidal effects of UV-C irradiation on SARS-CoV-2 were experimentally evaluated for different illumination doses and virus concentrations (1000, 5, 0.05 MOI). At a density comparable to that observed in infection, an dose just 3.7 mJ/cm 2 was sufficient achieve more than 3-log inactivation without any sign viral replication. Moreover, complete at all with 16.9 . These results could explain the epidemiological trends COVID-19 are important development novel...
Severe coronavirus disease 2019 (COVID-19) is characterized by overproduction of immune mediators, but the role interferons (IFNs) type I (IFN-I) or III (IFN-III) families remains debated. We scrutinized production IFNs along respiratory tract COVID-19 patients and found that high levels IFN-III, to a lesser extent IFN-I, characterize upper airways with viral burden reduced risk severity. Production specific not members denotes mild pathology efficiently drives transcription genes protect...
Gene transfer into HSCs is an effective treatment for SCID, although potentially limited by the risk of insertional mutagenesis. We performed a genome-wide analysis retroviral vector integrations in genetically corrected and their multilineage progeny before up to 47 months after transplantation 5 patients with adenosine deaminase–deficient SCID. Gene-dense regions, promoters, transcriptionally active genes were preferred sites (RISs) both preinfusion transduced CD34+ cells vivo gene...
Research Article5 November 2015Open Access Source Data Intra-arterial transplantation of HLA-matched donor mesoangioblasts in Duchenne muscular dystrophy Giulio Cossu Corresponding Author Institute Inflammation and Repair, University Manchester, UK Search for more papers by this author Stefano C Previtali Experimental Neurology (InSpe), Division Neuroscience, IRCCS San Raffaele Scientific Institute, Milan, Italy Department Neurology, Sara Napolitano HSR/TIGET Pediatric Clinical Unit,...
The clinical utility of new optical coherence tomography (OCT) instruments strongly depends on measurements reproducibility. aim this study was to assess retinal nerve fiber layer (RNFL) thickness reproducibility using six different spectral-domain OCTs (SD-OCTs) and one time-domain OCT.RNFL (average four quadrant) from SD-OCTs (Spectral OCT/SLO OPKO/OTI, 3D-OCT 2000 Topcon, RS-3000 NIDEK, Cirrus HD-OCT Zeiss, RTVue-100 Optovue, Spectralis Heidelberg) OCT (Stratus Zeiss) measured twice in 38...
Pyrazinamide (PZA) is a prodrug that converted to pyrazinoic acid by the enzyme pyrazinamidase, encoded pncA gene in Mycobacterium tuberculosis. Molecular identification of mutations offers potential for rapid detection pyrazinamide resistance (PZA(r)). However, genetic variants are highly variable and scattered over full length pncA, complicating development molecular test. We performed large multicenter study assessing sequence variations 1,950 clinical isolates, including 1,142...
T-cell therapy after hematopoietic stem cell transplantation (HSCT) has been used alone or in combination with immunosuppression to cure hematologic malignancies and prevent disease recurrence. Here, we describe the outcome of patients high-risk/advanced stage malignancies, who received depleted (TCD) haploidentical-HSCT (haplo-HSCT) combined donor T lymphocytes pretreated IL-10 (ALT-TEN trial). IL-10-anergized cells (IL-10-DLI) contained regulatory type 1 (Tr1) specific for host...
Alzheimer's disease (AD) and frontotemporal dementia (FTD) are leading causes of cognitive decline. Optical coherence tomography (OCT) allows the measurement thickness retinal neuroaxonal layers. While in AD mild impairment (MCI), nerve fiber layer (RNFL) thinning is frequently reported, less information available on ganglion cell layer-inner plexiform (GCL-IPL). Data FTD lacking.To obtain cross-sectional RNFL GCL-IPL among MCI, AD, FTD, healthy controls (HC), their correlations with...
Antigen exposure and differentiation phenotype influence long-term persistence of memory T cells after hematopoietic stem cell transplant.
The aim of this study was to evaluate factors associated with pathologic tumor response following pre-operative chemoradiation therapy, and the prognostic impact on overall disease-free survival.Between 1994 2002, 132 patients underwent therapy followed by surgery for middle lower rectal cancer. After excluding 26 cases (metastatic cancer, n = 13; nonradical surgery, 6; local excision procedure, 4; non-5-fluorouracil-based chemotherapy, 2; incomplete data preoperative regimen used, 1),...
Cystic changes in metastatic cervical lymph nodes (CLN) from papillary thyroid cancer (PTC) may be a diagnostic pitfall fine-needle aspiration biopsy (FNAB) cytology. We investigated series of CLN metastases cancers (TC), including cystic PTC, and wide spectrum extrathyroidal malignancies, the role for TC rapid detection thyroglobulin eluates FNAB (FNAB-Tg) CLN. The study was carried out group 79 subjects (22/57 M/F; median age, 56 years; range, 20-86 years) with enlarged nodules (TN),...
See also: Commentaire de travail S. Carrara et al., pp. 321Endoscopy 2008; 40(04): 453-453DOI: 10.1055/s-0032-1306829
To describe the magnetic resonance (MR) imaging and diffusion-weighted (DW) features of ocular adnexal lymphomas (OALs), to determine diagnostic accuracy apparent diffusion coefficient (ADC) for discriminating OALs from other orbital mass lesions, assess whether variations in ADC constitute a reliable biomarker OAL response therapy.Institutional ethical committee approval informed consent were obtained. In this prospective study, 114 white subjects (65 females 49 males) enrolled....
The analysis of genomic distribution retroviral vectors is a powerful tool to monitor 'vector-on-host' effects in gene therapy (GT) trials but also provides crucial information about 'host-on-vector' influences based on the target cell genetic and epigenetic state. We had unique occasion compare insertional profile same therapeutic moloney murine leukemia virus (MLV) vector context adenosine deaminase-severe combined immunodeficiency (ADA-SCID) background two GT infusions transduced mature...
Only few small RNAs (sRNAs) have been characterized in Mycobacterium tuberculosis and their role regulatory networks is still poorly understood. Here we report a genome-wide characterization of sRNAs M. integrating experimental computational analyses. Global RNA-seq analysis exponentially growing cultures H37Rv had previously identified 1373 sRNA species. In the present show that 258 (19%) these were also by microarray expression. This set included 22 intergenic sRNAs, 84 mapping within...