A5082117767- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- HIV-related health complications and treatments
- HIV/AIDS drug development and treatment
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Pneumocystis jirovecii pneumonia detection and treatment
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Impact on Reproduction
- COVID-19 Clinical Research Studies
- Infection Control and Ventilation
- Long-Term Effects of COVID-19
- Systemic Lupus Erythematosus Research
- Pediatric health and respiratory diseases
- Transplantation: Methods and Outcomes
- Tryptophan and brain disorders
- Monoclonal and Polyclonal Antibodies Research
- Reproductive System and Pregnancy
- Cervical Cancer and HPV Research
- COVID-19 epidemiological studies
- Respiratory viral infections research
- Immune Response and Inflammation
University of Milan
2016-2025
Luigi Sacco Hospital
2013-2022
ASST Fatebenefratelli Sacco
2021
Nerviano Medical Sciences
2016
Mylan (Switzerland)
2015
Milano University Press
2015
Don Carlo Gnocchi Foundation
1999-2012
Regione Puglia
2011
Istituto Nazionale di Fisica Nucleare, Laboratori Acceleratori e Superconduttività Applicata
2011
Abbott Fund
2009
Abstract Antigen- and mitogen-stimulated cytokine production by peripheral blood mononuclear cells (PBMC) of 50 pregnant women 31 age- sex-matched non-pregnant controls were analysed to determine whether changes in occur during normal pathologic human gestation. The women, consecutively enrolled a 3-month period, undergoing normal, non-pathologic pregnancy at the time entry into study, underwent ultrasound examination ascertain exact week vitality fetus. Forty pregnancies (80%) terminated...
Abstract The impact of SARS-CoV-2 infection during gestation remains unclear. Here, we analyse the viral genome on maternal and newborns nasopharyngeal swabs, vaginal umbilical cord plasma, placenta biopsies, amniotic fluids milk from 31 mothers with infection. In addition, also test specific anti-SARS-CoV-2 antibodies expression genes involved in inflammatory responses placentas, plasma. We detect one blood two at-term mucosa specimen. Furthermore, report presence IgM IgG Finally, three...
Abstract The potential virucidal effects of UV-C irradiation on SARS-CoV-2 were experimentally evaluated for different illumination doses and virus concentrations (1000, 5, 0.05 MOI). At a density comparable to that observed in infection, an dose just 3.7 mJ/cm 2 was sufficient achieve more than 3-log inactivation without any sign viral replication. Moreover, complete at all with 16.9 . These results could explain the epidemiological trends COVID-19 are important development novel...
Abstract COVID‐19 commonly leads to respiratory issues, yet numerous patients also exhibit a diverse range of neurological conditions, suggesting detrimental impact SARS‐CoV‐2 or the viral Spike protein on central nervous system. Nonetheless, molecular pathway behind pathology and presumed neurotropism remains largely unexplored. We generated human cortical organoids (HCOs) derived from induced pluripotent stem cells (hiPSC) assess: (1) expression main entry factors; (2) their vulnerability...
Objectives: Most HIV-1 transmission is sexual; therefore, immune responses in the genital mucosa may be important mediating protection against HIV infection. This study examined HIV-1-specific mucosal IgA a cohort of HIV-1-resistant Kenyan female sex workers. Methods: were compared and HIV-1-infected workers, lower risk uninfected women. Cervical vaginal samples from each group tested for IgG by enzyme immunoassay. Systemic T-helper lymphocyte cell to envelope peptide epitopes assayed using...
Background: Genital infection with certain strains of human papillomavirus (HPV) is associated a high risk malignant transformation, and HPV-associated cervical intraepithelial neoplasia (CIN) can become invasive cancer. Host factors are critical in regulating tumor growth, cytokines that modulate immunologic control may be particular importance. The type 1 interleukin 2 (IL-2) interferon gamma (IFN γ) immunostimulatory thus capable limiting growth. 4 (IL-4) 10 (IL-10) immunoinhibitory...
Intestinal mucosal immune system is an early target for human immunodeficiency virus type 1 (HIV-1) infection, resulting in CD4+ T-cell depletion, deterioration of gut lining, and fecal microbiota composition. We evaluated the effects a prebiotic oligosaccharide mixture highly active antiretroviral therapy (HAART)-naive HIV-1-infected adults. In pilot double-blind, randomized, placebo-controlled study, 57 HAART-naive patients received unique (15 or 30 g short chain...
Several cohort studies reported a relation of cardiovascular events and periodontal disease. In particular, Porphyromonas gingivalis is associated with the development atherosclerotic plaques. We verified in longitudinal study whether inflammation biomarkers, endothelial adhesion molecules, leukocyte activation markers, intima-media thickness could be beneficially modified by treatment alone. Thirty-five otherwise healthy individuals affected mild to moderate parodontopathy were enrolled...
Persistently reduced CD4(+) T-lymphocyte counts in the face of undetectable HIV viremia are seen a sizable percentage HIV-infected patients undergoing antiretroviral therapy (ART). We analyzed immune correlates this phenomenon.Sixty-seven with (<50 copies/microl) after more than 7 years ART were enrolled study and divided into two groups (CD4 cell >500 cells/microl or <500 cells/microl). Duration infection (>16 years) was comparable. Peripheral blood mononuclear stimulated gag+env...
Background: It has been suggested that probiotics modulate atopic dermatitis (AD) progression, but no data are actually available on their mechanisms of action and ability to act as immunomodulators in this pathology. Objective: The aim randomized double-blinded active treatment versus placebo study was evaluate clinical efficacy an intake a combination 2 (Lactobacillus salivarius LS01 Bifidobacterium breve BR03) for the adult AD patients. Methods: Forty-eight patients were enrolled...
Inflammasome-mediated activation of caspase-1 regulates inflammatory responses and pyroptosis. We analyzed possible associations between inflammasome-related genes immune reconstitution in HIV-infected antiretroviral therapy (ART)-treated patients.Cross-sectional, case-control study. patients on ART for ≥24 months with HIV-RNA<50 cp/mL ≥12 were enrolled defined as immunological responders (IR) or non-responders (INR) if CD4 count was ≥500 ≤350 cells/μL, respectively. Expression inflammasome...
The reason behind the high inter-individual variability in response to SARS-CoV-2 infection and patient’s outcome is poorly understood. present study targets sphingolipid profile of twenty-four healthy controls fifty-nine COVID-19 patients with different disease severity. Sera were analyzed by untargeted targeted mass spectrometry ELISA. Results indicated a progressive increase dihydrosphingosine, dihydroceramides, ceramides, sphingosine, decrease sphingosine-1-phosphate. These changes are...
Background Acquired HIV-specific cell-mediated immune responses have been observed in exposed–uninfected individuals, and it has inferred, but not demonstrated, that these constitute a part of natural protective immunity to HIV. This inference was tested prospectively the exposure setting maternal–infant HIV transmission predominantly breast-fed population. Methods Cord blood from infants HIV-seropositive women Durban, South Africa, were for vitro reactivity cocktail envelope peptides (Env)...
To provide insight into the dynamics and source of residual viremia in human immunodeficiency virus (HIV) patients successfully treated with antiretroviral therapy, 14 intensely monitored indinavir efavirenz sustaining HIV RNA at <50 copies/ml for >5 years were studied. Abacavir was added to regimen eight year 5. After first 9 months levels had reached a plateau ("residual viremia") that persisted over 5 years. Levels differed among ranged from 3.2 23 copies/ml. Baseline DNA only significant...
The presence and activity of human immunodeficiency virus (HIV)-specific antibodies were analyzed in the sera 15 sexually exposed seronegative persons who had systemic HIV-specific cell-mediated immunity IgA-mediated mucosal their HIV-infected partners. HIV-positive subjects serum IgG IgA; IgA absence IgG. Testing 1 year after interruption at-risk sex showed that no seroconversion occurred concentrations declined. Serum from HIV-exposed was for ability to neutralize primary HIV-1 isolates....
Rational design of DC-SIGN antagonists combines a selective glycomimetic ligand with trivalent dendrons spaced by rigid core and allows to exploit multiple multivalency effects.
Abstract Background Increasing evidence suggests a double-faceted role of alpha-synuclein (α-syn) following infection by variety viruses, including SARS-CoV-2. Although α-syn accumulation is known to contribute cell toxicity and the development and/or exacerbation neuropathological manifestations, it also key sustaining anti-viral innate immunity. Consistently with aggregation as hallmark Parkinson's disease, most studies investigating biological function focused on neural cells, while...
There is an urgent need for a universally effective HIV-1 vaccine, but whether vaccine will be able to protect against of different clades significant concern. IgA from HIV-1-exposed, persistently seronegative (HEPS) subjects has been shown neutralize and block epithelial transcytosis, it may target novel epitopes. We have tested the ability plasma mucosal purified HEPS primary isolates viral phenotypes. two groups was tested: sex workers Nairobi, Kenya, where A D predominate, heterosexual...
Background HIV infection in Africa is associated with immune activation and a cytokine profile that stimulates CCR5 expression. We investigated whether this environmentally driven; if dominant expression of could indeed be detected African individuals; R5 strains would prevalent population. Methods Freshly drawn peripheral blood mononuclear cells from HIV-uninfected Italian individuals living rural Africa, Africans Italians Italy, HIV-infected patients were analysed. Determinations...