A5062140006- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Poxvirus research and outbreaks
- Hepatitis C virus research
- Bacillus and Francisella bacterial research
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- HIV/AIDS Research and Interventions
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Pregnancy and Medication Impact
- Reproductive System and Pregnancy
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- COVID-19 epidemiological studies
- Systemic Lupus Erythematosus Research
- Pregnancy and preeclampsia studies
- COVID-19 and healthcare impacts
- Trypanosoma species research and implications
- Liver Disease Diagnosis and Treatment
- Research on Leishmaniasis Studies
ASST Fatebenefratelli Sacco
2017-2025
Luigi Sacco Hospital
2013-2025
University of Milan
2013-2022
Abstract The impact of SARS-CoV-2 infection during gestation remains unclear. Here, we analyse the viral genome on maternal and newborns nasopharyngeal swabs, vaginal umbilical cord plasma, placenta biopsies, amniotic fluids milk from 31 mothers with infection. In addition, also test specific anti-SARS-CoV-2 antibodies expression genes involved in inflammatory responses placentas, plasma. We detect one blood two at-term mucosa specimen. Furthermore, report presence IgM IgG Finally, three...
Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In present study, raw treated samples from three wastewater treatment plants, two river characterized Milano Metropolitan Area, Italy, were surveyed for RNA positivity real time PCR infectiveness. Moreover, whole genome sequencing phylogenetic analysis isolated strains was performed. Raw resulted positive amplification, while water always negative (four samples, respectively, sampled in...
Chandipura virus (CHPV) is an arthropod-borne linked to encephalitis in humans, primarily India. Its evolutionary dynamics and transmission pathways remain poorly understood due limited genomic data. This study analyzed 23 publicly available CHPV genomes, including isolates from sandflies, a hedgehog, retrieved GenBank. Phylogenetic analyses were conducted explore host-specific geographic patterns. analysis revealed distinct lineages. Human-derived genomes collected India between 2003 2024...
As the SARS-CoV-2 pandemic continues to rage worldwide, emergence of numerous variants concern (VOC) represents a challenge for vaccinal protective efficacy and reliability commercially available high-throughput immunoassays. Our study demonstrates administration two doses BNT162b2 vaccine that elicited robust SARS-CoV-2-specific immune response which was assessed up 3 months after full vaccination in cohort 37 health care workers (HCWs). antibody response, evaluated by four...
A diluted 3% w/w hydrogen peroxide solution acidified to pH 2.5 by adding citric acid inactivated SARS-CoV-2 virus more than 4 orders of magnitude in 5 min. After a contact time 15 min, no viral replication was detected. Aqueous solutions sodium percarbonate coronavirus >3 log10 diminution Conversely, H2O2 with additives displayed scarce virucidal activity (1.1 min), confirming that pH-modifying ingredient is necessary have H2O2-based disinfectant active against the novel coronavirus.
Monkeypox virus (MPXV) infection confirmation needs reliable polymerase chain reaction (PCR) assays; in addition, viral clade attribution is a key factor containment measures, considering more severe syndrome I and possibility of simultaneous circulation. This study aims to evaluate the performance all-in-one STANDARD M10 MPX/OPX (SD BIOSENSOR, South Korea – M10). Frozen samples from 205 subjects were selected stratified according routine test results (RealStar® Orthopoxvirus PCR Kit 1.0,...
Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% cases. The present aims to: i) evaluate presence and shedding duration MPXV DNA different body compartments correlating viability with time to onset symptoms; ii) provide evidence persistence compartment as a source infection iii) characterize evolution by whole genome sequencing (WGS) during...
Arboviral diseases are a growing global health concern. Pre-existing autoantibodies (auto-Abs) neutralizing type I interferons (IFNs) can underlie encephalitis due to West Nile virus (WNV) (∼40% of patients) and tick-borne (TBE, TBE [TBEV]) (∼10%). We report here that these auto-Abs also severe forms rarer arboviral infections. Auto-Abs high concentrations IFN-α2, IFN-β, and/or IFN-ω present in the single case Powassan (POWV) studied, two three cases Usutu (USUV) infection most 24 Ross River...
We identified the first case in Italy of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) B.1.1.529 variant, using whole-genome sequencing an Italian subject traveling from Mozambique. Specific mutation profiles deserve further investigations to clarify potential effects on vaccination efficacy. This highlights crucial role rapid and continuous surveillance SARS-CoV-2 variant circulation.
In 2022, many monkeypox (MPX) outbreaks have been documented in countries where MPX was not endemic. It spread all around the world, especially European Union and United States. While is classically considered to be transmitted through close contact with lesions, hypothesis of sexual transmission has proposed. This study a total 49 patients suspected for that were also tested other sexually infections (STIs), including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium,...
We describe two cases of Oropouche fever in travellers from Brazil, late March 2024, and Cuba, July 2024. The virus was carried outside the Americas before Cuba outbreak, requiring screening febrile Latin America. Studies on possible vectors reservoirs new areas should be implemented.
Abstract The first identification of autochthonous transmission SARS‐CoV‐2 in Italy was documented by the Laboratory Clinical Microbiology, Virology and Bioemergencies L. Sacco Hospital (Milano, Italy) on 20th February 2020 a 38 years old male patient, who found positive for pneumonia at Codogno Hospital. Thereafter Lombardy has reported highest prevalence COVID‐19 cases country, especially Milano, Brescia Bergamo provinces. aim this study to assess potential presence different viral...
Background: We assessed the impact of coronavirus disease 2019 (COVID-19) pandemic on HIV suppression rates in people living with (PLWH) attending a large Italian clinic. Setting: The outpatient clinic Infectious Diseases Department Luigi Sacco Hospital, Milan, Italy, which serves more than 5000 PLWH per year. Methods: A before and after quasi -experimental study design was used to make retrospective assessment monthly trend HIV-RNA determinations ≥50 among our clinic, “before” being period...
To assay the presence of SARS-CoV-2 genome in vaginal, rectal, and placental swabs among pregnant women newborn nasopharyngeal to investigate immunological response maternal antibody transfer through umbilical cord blood milk unvaccinated mothers.Vaginal, specimens, neonatal serum, were collected from a wide cohort Italian with confirmed infection admitted hospital between February 25, 2020 June 30, 2021. Samples tested selected reference laboratories according shared interlaboratory...
A natural monkeypox virus infection may not induce sufficient neutralizing antibody responses in a subset of healthy individuals. The aim this study was to evaluate virus-neutralizing antibodies six months after and assess the virological factors predictive poor immunological response. Antibodies were assessed using plaque reduction neutralization test at from mpox infection; cutaneous, oropharyngeal, anal swabs, semen, plasma samples tested during infection. Overall, 95 people included...
Abstract Pregnancy is known to increase the risk of severe illnesses in response viral infections. Therefore, impact SARS-CoV-2 infection during gestational ages might be detrimental and potential vertical transmission should thoroughly studied.Herein, we investigated whether possible and, case, this results a fetal involvement. Additionally, analyzed role antibody inflammatory responses placenta plasma from SARS-CoV-2-positive pregnant women fetuses.31 were enrolled. Real-time PCR was...