A5028287921- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Outbreaks Research
- Zoonotic diseases and public health
- Malaria Research and Control
- Respiratory viral infections research
- Vector-borne infectious diseases
- Orthopedic Infections and Treatments
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Microbial Community Ecology and Physiology
- Insect symbiosis and bacterial influences
- Dengue and Mosquito Control Research
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Infectious Diseases and Tuberculosis
- Microbial infections and disease research
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Virology and Viral Diseases
Aix-Marseille Université
2016-2025
Inserm
2016-2025
Institut de Recherche pour le Développement
2016-2024
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2016-2024
Assistance Publique Hôpitaux de Marseille
2016-2024
Institut de Médecine Tropicale du Service de Santé des Armées
2020-2024
Centre National de la Recherche Scientifique
2016-2024
Laboratoire National de Référence
2024
Biomedical Research Institute
2023
Hôpital d'Instruction des Armées Bégin
2023
Abstract Metagenomics revolutionized the understanding of relations among human microbiome, health and diseases, but generated a countless number sequences that have not been assigned to known microorganism 1 . The pure culture prokaryotes, neglected in recent decades, remains essential elucidating role these organisms 2 We recently introduced microbial culturomics, culturing approach uses multiple conditions matrix-assisted laser desorption/ionization–time flight 16S rRNA for identification...
In 2023, dengue virus serotype 2 (DENV2) affected most French overseas territories. the Caribbean Islands, viral circulation continues with > 30,000 suspected infections by March 2024. Genome sequence analysis reveals that epidemic lineage in islands has also become established Guiana but not Réunion. It moreover seeded autochthonous events mainland France. To guide prevention of further inter-territorial spread and DENV introduction non-endemic settings, continued molecular surveillance...
Tick-borne encephalitis virus' (TBEV) geographic range and the human incidence are increasing throughout Europe, putting a number of non-endemic regions countries at risk outbreaks. In spring 2020, there was an outbreak tick-born (TBE) in Ain, Eastern France, where virus had never been detected before. All patients but one consumed traditional unpasteurised raw goat cheese from local producer. We conducted investigation suspected farm using integrative One Health approach. Our methodology...
ABSTRACT Background Japanese encephalitis virus (JEV) is a mosquito-borne pathogen responsible for (JE) clinical cases in Asia and the Western Pacific. Non-JE-vaccinated patients can develop potentially life-threatening neurologic forms. Case Summary Here, we present two severe, non-fatal JEV returning travelers from Cambodia (Case 1) Vietnam 2), imported to France 2023–2024. Neither patient was vaccinated, both presented symptoms requiring hospitalization. Cases were confirmed by RT-qPCR,...
Chikungunya virus (CHIKV) is known to circulate in Africa, but there little evidence of CHIKV transmission Côte d’Ivoire. Using genomic data, we linked cases imported France from d’Ivoire a lineage the West African genotype, likely been circulating country since July 2021.
The identification of "asymptomatic" (i.e., protective) epitopes recognized by T cells from herpes simplex virus (HSV)-seropositive healthy individuals is a prerequisite for an effective vaccine. Using the PepScan epitope mapping strategy, library 179 potential peptide (15-mers overlapping 10 amino acids) was identified HSV type 1 (HSV-1) glycoprotein B (gB), antigen that induces protective immunity in both animal models and humans. Eighteen groups (G1 to G18) adjacent peptides each were...
An anaerobic bacterium, strain AT2T, was isolated from the fresh stool sample of a healthy French man using culturomics approach. The 16S rRNA gene sequence analysis showed that AT2T had 95.2 % nucleotide similarity with Gemmiger formicilisATCC 27749T, phylogenetically closest species standing in nomenclature. Cells are Gram-stain-negative, catalase- and oxidase-negative, obligately anaerobic, non-motile, non-spore-forming, rod-shaped, bacilli were mesothermophilic. major fatty acids C16 : 0...
Since 2015, annual West Nile virus (WNV) outbreaks of varying intensities have been reported in France. Recent intensification enzootic WNV circulation was observed the South France with most horse cases detected 2015 (n = 49), 2018 13), and 2019 13). A lineage 1 strain isolated from a suffering neuro-invasive disease (WNND) during episode Camargue area. breaking point epidemiology achieved 2018, when 2 emerged Southeastern areas. This probably originated spread Northern Italy caused WNND...
BackgroundBronchiolitis caused by the respiratory syncytial virus (RSV) in infants less than two years old is a growing public health concern worldwide, and there currently no safe effective vaccine. A major component of RSV nucleocapsid, nucleoprotein (N), has been so far poorly explored as potential vaccine antigen, even though it target protective anti-viral T cell responses remarkably conserved between human B serotypes. We recently reported method to produce recombinant N assembling...
Outbreaks of Kingella kingae infection are an emerging public health concern among daycare attendees carrying epidemic clones in the oropharynx. However, genotyping such from affected cases is limited by low performance current methods to detect K. blood samples and lack specimens available infected sites. We aimed at developing a modified multilocus sequence typing (MLST) method genotype strains oropharyngeal without prior culture. designed silico MLST primers specific for aligning whole...
The recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has highlighted the importance reliable and rapid diagnostic testing to prevent control virus circulation. Dozens monoplex in-house RT-qPCR assays are already available; however, development dual-target is suited avoid false-negative results caused by polymorphisms or point mutations, that can compromise accuracy screening tests. In this study, two mono-target recommended WHO (E-Sarbeco (enveloppe...
Emerging and endemic mosquito-borne viruses can be difficult to detect monitor because they often cause asymptomatic infections in human or vertebrate animals nonspecific febrile illness with a short recovery waiting period. Some of these pathogens circulate into complex cryptic cycles involving several animal species as reservoir amplifying hosts. Detection cases hosts complemented by entomological surveillance, but this method is not adapted low infection rates mosquito populations that...
is a significant pediatric pathogen responsible for bone and joint infections, occult bacteremia, endocarditis in early childhood. Past efforts to detect this bacterium using culture broad-range 16S rRNA gene PCR assays from clinical specimens have proven unsatisfactory; therefore, by the late 2000s, these were gradually phased out explore benefits of specific real-time tests targeting
Intestinimonas massiliensis sp. nov strain GD2T is a new species of the genus (the second, following butyriciproducens gen. nov., nov). First isolated from gut microbiota healthy subject French origin using culturomics approach combined with taxono-genomics, it strictly anaerobic, nonspore-forming, rod-shaped, catalase- and oxidase-negative reactions. Its growth was observed after preincubation in an anaerobic blood culture enriched sheep (5%) rumen fluid (5%), incubated at 37°C. phenotypic...
The presence of alphaviruses, such as chikungunya virus (CHIKV), has never been reported in Mauritania. We assessed the seroprevalence CHIKV among Nouakchott residents. A cross-sectional study involving 1300 non-febrile patients consulting at hospital center was conducted between January and June 2021. anti-CHIKV IgG neutralizing antibodies against CHIKV, O’nyong-nyong (ONNV), Semliki Forest (SFV) determined by an enzyme-linked immunosorbent assay (ELISA) a serum neutralization test,...
Abstract Background Yellow fever vaccine exists for over 80 years and is considered to be relatively safe. However, in rare cases it can produce serious neurotropic viscerotropic complications. We report a case of patient who presented both neurological manifestations after yellow vaccination. Case presentation describe the 37 old man developed vaccination vaccine-associated disease followed by acute uveitis. Prolonged detection RNA blood urine was consistent with adverse event. The final...
Three autochthonous cases of Zika virus occurred in southern France August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for isolation ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed issue genotyping geographical origin by performing hemi-nested PCR sequencing Pr gene. Analysis 16 genotype-specific Single Nucleotides Polymorphisms identified Asian genotype suggested a Southeast Asia origin.
Abstract Zika virus has been circulating in Thailand since 2002 through continuous but likely low-level circulation. Here, we describe an infection a pregnant woman who traveled to and South America during her pregnancy. By combining phylogenetic analysis with the patient's travel history pregnancy timeline, confirmed that she got infected at end of 2021. This imported case microcephaly highlights circulation country still constitutes health risk, even year lower incidence. Main points Here...
Abstract Background Immunization against the Yellow fever virus (YFV) with 17D live-attenuated vaccine is most effective way to prevent disease. However, unexpected severe adverse events can occur. They consist in a neurological impairment - disease (YEL-AND), YF-like illness viscerotropic (YEL-AVD) or anaphylaxis. In this article, we describe epidemiology, clinical and biological features of YEL-AND YEL-AVD cases reported French National Reference Center for Arboviruses (NRCA) past 10...
ABSTRACT Yersinia pestis is the causative agent of plague, a rapidly fatal infectious disease that has not been eradicated worldwide. The capsular Caf1 protein Y. protective antigen under development as recombinant vaccine. However, little known about specificity human T-cell responses for Caf1. We characterized CD4 epitopes in “humanized” HLA-DR1 transgenic mice lacking endogenous major histocompatibility complex class II molecules. Mice were immunized with or each complete set overlapping...