A5033929355- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Malaria Research and Control
- Vector-borne infectious diseases
- Dengue and Mosquito Control Research
- Insect Resistance and Genetics
- Zoonotic diseases and public health
- Invertebrate Immune Response Mechanisms
- COVID-19 epidemiological studies
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Reproductive System and Pregnancy
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Identification and Susceptibility Testing
- HIV Research and Treatment
- Insect Pest Control Strategies
- Evolution and Genetic Dynamics
- Insect behavior and control techniques
- Viral Infections and Outbreaks Research
- Complement system in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Forest Insect Ecology and Management
- Genetics, Bioinformatics, and Biomedical Research
- Estrogen and related hormone effects
Institut de Médecine Tropicale du Service de Santé des Armées
2016-2025
Inserm
2016-2025
Aix-Marseille Université
2016-2025
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2008-2024
Institut de Recherche pour le Développement
2016-2024
Méditerranée Infection Foundation
2016-2024
Service de Santé des Armées
2023
Institut Pasteur
2013-2020
Centre National de la Recherche Scientifique
2015-2020
Department of Genomes & Genetics
2016-2018
Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of developing tools to fight them has been slowed by lack a high-quality genome assembly. Here we combine diverse technologies produce markedly improved, fully re-annotated AaegL5 assembly, demonstrate how it accelerates mosquito science. We anchored physical cytogenetic maps, doubled number...
Abstract The global emergence of Zika virus (ZIKV) revealed the unprecedented ability for a mosquito-borne to cause congenital birth defects. A puzzling aspect ZIKV is that all human outbreaks and defects date have been exclusively associated with Asian lineage, despite growing body laboratory evidence pointing towards higher transmissibility pathogenicity African lineage. Whether this apparent paradox reflects use relatively old strains in most studies unclear. Here, we experimentally...
Interactions between pathogens and their insect vectors in nature are under the control of both genetic non-genetic factors, yet most studies on mosquito vector competence for human conducted laboratory systems that do not consider and/or environmental variability. Evaluating risk emergence arthropod-borne viruses (arboviruses) public health importance such as chikungunya virus (CHIKV) requires a more realistic appraisal contributions to competence. In particular, sources variation...
Due to their error-prone replication, RNA viruses typically exist as a diverse population of closely related genomes, which is considered critical for fitness and adaptive potential. Intra-host demographic fluctuations that stochastically reduce the effective size viral populations are challenge maintaining genetic diversity during systemic host infection. Arthropod-borne (arboviruses) traverse several anatomical barriers infection arthropod vectors believed impose bottlenecks. These have...
Specific interactions between host genotypes and pathogen (G×G interactions) are commonly observed in invertebrate systems. Such specificity challenges our current understanding of defenses against pathogens because it contrasts the limited discriminatory power known immune responses. Lack a mechanistic explanation, however, has questioned nature factors underlying G×G interactions. In this study, we aimed to determine whether dengue viruses their Aedes aegypti vectors can be mapped discrete...
Endogenous viral elements (EVEs) are sequences integrated in host genomes. A large number of non-retroviral EVEs was recently detected Aedes mosquito genomes, leading to the hypothesis that may control exogenous infections by closely related viruses. Here, we experimentally investigated role an EVE naturally found aegypti populations and derived from widespread insect-specific virus, cell-fusing agent virus (CFAV). Using CRISPR-Cas9 genome editing, created Ae. line lacking CFAV EVE. Absence...
In 2023, dengue virus serotype 2 (DENV2) affected most French overseas territories. the Caribbean Islands, viral circulation continues with > 30,000 suspected infections by March 2024. Genome sequence analysis reveals that epidemic lineage in islands has also become established Guiana but not Réunion. It moreover seeded autochthonous events mainland France. To guide prevention of further inter-territorial spread and DENV introduction non-endemic settings, continued molecular surveillance...
Cerebral malaria (CM) is characterized by accumulation of circulating cells within brain microvessels, among which platelets play an important role. In vitro, modulate the cytoadherence Plasmodium falciparum-parasitized red blood (PRBCs) to endothelial cells. Here we show for first time that platelet microparticles (PMPs) are able bind PRBCs, thereby transferring antigens PRBC surface. This binding largely specific because PMPs little adherence normal PMP also dependent on P. falciparum...
Successful transmission of a vector-borne pathogen relies on complex life cycle in the arthropod vector that requires initial infection digestive tract followed by systemic viral dissemination. The time interval between acquisition and subsequent pathogen, called extrinsic incubation period, is one most influential parameters transmission. However, dynamic nature this process often ignored because competence assays are sacrificial rely end-point measurements. Here, we report individual Aedes...
To identify potential sylvatic, urban and bridge-vectors that can be involved in current or future virus spillover from wild to more urbanised areas, entomological field surveys were conducted rural, peri-urban areas spanning the rainy dry seasons western Cameroon. A total of 2650 mosquitoes belonging 37 species eight genera collected. Mosquito richness was significantly influenced by specific combination habitat type season. The highest found area (S = 30, Chao1 121 ± 50.63, ACE 51.97 3.88)...
Abstract Background The emergence of Plasmodium falciparum resistance to most anti-malarial compounds has highlighted the urgency develop new drugs and clarify mechanisms currently used. Among them, doxycycline is used alone for malaria chemoprophylaxis or in combination with quinine artemisinin derivatives treatment. molecular action P. have not yet been clearly defined, particularly at protein level. Methods A proteomic approach was analyse expression changes schizont stage malarial...
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology (MALDI-TOF MS) is an innovative tool that has been shown to be effective for the identification of numerous arthropod groups including mosquitoes. A critical step in implementation MALDI-TOF MS creation spectra databases (DB) species interest. Mosquito legs were body part most frequently used create DB. However, are one fragile mosquito compartments, which can put at risk. Here, we assessed whether...
Mechanisms and evolutionary dynamics of sex-determination systems are particular interest in insect vectors human pathogens like mosquitoes because novel control strategies aim to convert pathogen-transmitting females into nonbiting males, or rely on accurate sexing for the release sterile males. In Aedes aegypti, main vector dengue Zika viruses, sex determination is governed by a dominant male-determining locus, previously thought reside within small, nonrecombining, sex-determining region...
The kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant arbovirus epidemiology. time interval between acquisition and the vector, termed extrinsic incubation period (EIP), combines with vector mortality rate competence to determine proportion infected that eventually become infectious. However, dynamic nature this process, amount natural variation in among strains, are poorly documented empirically rarely considered...
Abstract Chemical control of disease vectoring mosquitoes Aedes albopictus and aegypti is costly, unsustainable, increasingly ineffective due to the spread insecticide resistance. The Sterile Insect Technique a valuable alternative but limited by slow, error-prone, wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each species) based on fluorescence markers linked m M sex loci, allowing isolation transgenic males. Furthermore, demonstrate how combining...
Mosquito-borne diseases are major health problems worldwide. Serological responses to mosquito saliva proteins may be useful in estimating individual exposure bites from mosquitoes transmitting these diseases. However, the relationships between levels of IgG and density as well response specificity at genus and/or species level need clarified prior develop new immunological markers assess human/vector contact. To this end, a kinetic study antibody against several salivary gland extracts...
Chikungunya virus (CHIKV) is known to circulate in Africa, but there little evidence of CHIKV transmission Côte d’Ivoire. Using genomic data, we linked cases imported France from d’Ivoire a lineage the West African genotype, likely been circulating country since July 2021.
Abstract Global changes have profoundly altered the interactions between pathogens and their hosts accelerating emergence of infectious diseases. Monitoring vector-borne diseases is therefore challenging requires an upgrading detection system relying mainly nowadays on passive surveillance reactive measures when a human case diagnosed. West Nile virus (WNV) Usutu (USUV) are two zoonotic orthoflaviviruses, maintained bird populations mosquitoes, threatening public veterinary health in Europe....
The in vitro activity of ferroquine (FQ) (SR97193), a 4-aminoquinoline antimalarial compound that contains ferrocenic nucleus, against 15 Plasmodium falciparum strains was assessed and compared with those chloroquine (CQ), quinine (QN), monodesethylamodiaquine (MDAQ), mefloquine (MQ). These were genotyped for polymorphisms quinoline resistance-associated genes such as Pfcrt, Pfmdr1, Pfmrp, Pfnhe-1. FQ highly active CQ-resistant parasites or reduced susceptibility to QN, MDAQ, MQ....
Abstract Background Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into vertebrate host. In sub-Saharan Africa, most malaria is due to Anopheles funestus s.s and gambiae s.l. (mainly s.s. arabiensis ). Several studies have demonstrated that immune response against salivary antigens could be used evaluate individual exposure mosquito bites. The aim this study was assess use secreted proteins as specific biomarkers An...
Diseases caused by arthropod-borne viruses are a significant threat to the health of human and animal populations throughout world. Better knowledge molecules synthesized in salivary gland saliva hematophagous arthropods could be use for improving control pathogen transmission. Recently, sialome analysis three Aedes aegypti mosquito colonies (PAEA, Rockefeller, Formosus) carried out our laboratory allowed us identify 44 proteins. Of these secreted proteins, none was exclusively expressed one...
Originating from African forests, Zika virus (ZIKV) has now emerged worldwide in urbanized areas, mainly transmitted by Aedes aegypti mosquitoes. Although albopictus can transmit ZIKV experimentally and was suspected to be a vector Central Africa, the potential of this species sustain transmission yet uncovered until end 2019, when several autochthonous transmissions vectored Ae . occurred France. Aside these few locally acquired infections, most territories colonized have been spared so...