A5069920575- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Malaria Research and Control
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 detection and testing
- Bartonella species infections research
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Invertebrate Immune Response Mechanisms
- HIV Research and Treatment
- COVID-19 diagnosis using AI
- Dermatological diseases and infestations
- Trypanosoma species research and implications
- Monoclonal and Polyclonal Antibodies Research
- Identification and Quantification in Food
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Insect Resistance and Genetics
- interferon and immune responses
- Lepidoptera: Biology and Taxonomy
- Species Distribution and Climate Change
- Rabies epidemiology and control
- Insect symbiosis and bacterial influences
Méditerranée Infection Foundation
2016-2025
Institut de Médecine Tropicale du Service de Santé des Armées
2016-2025
Institut de Recherche pour le Développement
2016-2025
Aix-Marseille Université
2016-2025
Assistance Publique Hôpitaux de Marseille
2017-2024
Service de Santé des Armées
2024
Ministère des Armées
2022
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2008-2020
Centre National de la Recherche Scientifique
2006-2017
Inserm
2012-2017
An increased risk for multiple sclerosis and schizophrenia is observed at increasing latitude in patients born winter or spring. To explore a possible link between maternal vitamin D deficiency these brain disorders, we examined the impact of prenatal hypovitaminosis on protein expression adult rat brain. Vitamin D-deficient female rats were mated with normal males. Pregnant females kept until birth whereupon they returned to control diet. At week 10, progeny's prefrontal cortex hippocampus...
Ticks are considered the second vector of human and animal diseases after mosquitoes. Therefore, identification ticks associated pathogens is an important step in management these vectors. In recent years, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for arthropods including ticks. The objective this study was to improve conditions preparation tick samples their by MALDI-TOF MS from field-collected...
Bartonella quintana, the etiologic agent of trench fever and other human diseases, is transmitted by feces body lice. Recently, this bacterium has been detected in arthropod families such as bed bugs, which begs question their involvement B. quintana transmission. Although several infectious pathogens have reported are suggested to be evidence regarding competence vectors unclear.Bed bugs at adult instar developmental stages were fed three successive blood meals inoculated with from day one...
The identification of mosquito vectors is generally based on morphological criteria, but for aquatic stages, characteristics may be missing, leading to incomplete or incorrect identification. high cost molecular biology techniques requires the development an alternative strategy. In last decade, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has proved efficient arthropod at species level. To investigate usefulness MALDI-TOF MS...
Background Phlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans animals in many countries worldwide. Precise fly identification is essential prevent phlebotomine-borne diseases. Over the past two decades, progress matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of present study was investigate usefulness MALDI-TOF MS a...
Malaria is still a major public health issue worldwide, and one of the best approaches to fight disease remains vector control. The current methods for mosquito identification include morphological that are generally time-consuming require expertise, molecular laboratory facilities with relatively expensive running costs. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology, routinely used bacterial identification, has recently emerged in...
The rapid spread of vector-borne diseases demands the development an innovative strategy for arthropod monitoring. emergence MALDI-TOF MS as a rapid, low-cost, and accurate tool identification is revolutionizing medical entomology. However, spectra from can vary according to body part selected, sample homogenization method used mode duration storage, standardization protocols indispensable prior creation sharing reference database. In present study, manual grinding Anopheles gambiae Giles...
Arthropod vectors have historically been identified morphologically, and more recently using molecular biology methods. However, both of these methods are time-consuming require specific expertise equipment. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which has revolutionized the routine identification microorganisms in clinical microbiology laboratories, was successfully applied to arthropod vectors. Since then, robustness this technique confirmed, extended...
Abstract Background The emergence of Plasmodium falciparum resistance to most anti-malarial compounds has highlighted the urgency develop new drugs and clarify mechanisms currently used. Among them, doxycycline is used alone for malaria chemoprophylaxis or in combination with quinine artemisinin derivatives treatment. molecular action P. have not yet been clearly defined, particularly at protein level. Methods A proteomic approach was analyse expression changes schizont stage malarial...
Background Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been shown to be an effective tool for the rapid identification of arthropods, including tick vectors human diseases. Methodology/Principal Findings The objective present study was evaluate use MALDI-TOF MS identify species, and determine presence rickettsia pathogens in infected Ticks. Rhipicephalus sanguineus Dermacentor marginatus Ticks or not by R. conorii slovaca, respectively,...
The identification of blood meal sources in malaria vectors is critical to better understanding host/vector interactions and epidemiology control. Currently, the mosquito origins based on time-consuming costly techniques such as precipitin tests, ELISA molecular tools. Although these tools have been validated identify trophic preferences female Anopheles mosquitoes, they present several limitations. Recently, matrix-assisted, laser desorption/ionization time-of-flight mass spectrometry...
Background In Africa, relapsing fever borreliae are neglected vector-borne pathogens that cause mild to deadly septicemia and miscarriage. Screening vectors for the presence of currently requires technically demanding, time- resource-consuming molecular methods. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has recently emerged as a tool rapid identification cultured borreliae. We investigated whether MALDI-TOF-MS could detect directly in ticks....
Abstract Ticks and fleas are vectors for numerous human animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector non‐vector species. Recently, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry ( MALDI‐TOF‐MS) was applied the rapid identification of arthropods. The growth this promising tool, however, guidelines be established. To end, standardization protocols were species...
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology (MALDI-TOF MS) is an innovative tool that has been shown to be effective for the identification of numerous arthropod groups including mosquitoes. A critical step in implementation MALDI-TOF MS creation spectra databases (DB) species interest. Mosquito legs were body part most frequently used create DB. However, are one fragile mosquito compartments, which can put at risk. Here, we assessed whether...
Mosquitoes transmit a wide range of human parasitic and viral diseases. In recent years, new techniques such as MALDI-TOF MS have been developed to identify mosquitoes at the species level, which is key for entomological surveys. Additionally, there increasing interest in mosquito microbiota its role vector capacity.The culturomics approach previously used our laboratory study gut was applied evaluate midgut bacterial diversity Anopheles gambiae (wild strains), Aedes albopictus strains)...