A5026646845- HIV Research and Treatment
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Bartonella species infections research
- HIV/AIDS Research and Interventions
- Epigenetics and DNA Methylation
- Gut microbiota and health
- RNA Interference and Gene Delivery
- Yersinia bacterium, plague, ectoparasites research
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Telomeres, Telomerase, and Senescence
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2006-2025
Centre National de la Recherche Scientifique
2005-2025
Institut de Recherche pour le Développement
2011-2025
Méditerranée Infection Foundation
2017-2025
Aix-Marseille Université
2013-2025
Inserm
2001-2025
Institut Paoli-Calmettes
2019-2023
Institut Pprime
2021
Centre de Recherche en Cancérologie de Marseille
2021
Institut de Recherche pour le Développement
2012
A growing number of recent reports have implicated Rickettsia felis as a human pathogen, paralleling the increasing detection R. in arthropod hosts across globe, primarily fleas. Here Anopheles gambiae mosquitoes, primary malarial vectors sub-Saharan Africa, were fed with either blood meal infected or cellular media administered membrane feeding systems. In addition, group mosquitoes was on felis-infected BALB/c mice. The acquisition and persistence demonstrated by quantitative PCR bacteria...
Vibrio cholerae is the cause of diarrheal disease cholera. V. produces RtxA, a large toxin MARTX family, which targeted to host cell cytosol, where its actin cross-linking domain (ACD) cross-links G-actin, leading F-actin depolymerization, cytoskeleton rearrangements, and rounding. These effects on prevent phagocytosis bacterial engulfment by macrophages, thus preventing clearance from gut. The Type VI secretion-associated VgrG1 protein also contains C-terminal ACD, shares 61% identity with...
Abstract Exopolysaccharides produced by bacterial species and present in feces are extremely inhibitory to DNA restriction can cause discrepancies metagenomic studies. We determined the effects of different extraction methods on apparent composition gut microbiota using Illumina MiSeq deep sequencing technology. was extracted from stool an obese female 10 choice method affected proportional abundance at phylum level, richness (Chao index, 227 2,714) diversity (non parametric Shannon, 1.37...
Ubiquitin-fold modifier 1 (UFM1) is involved in neural and erythroid development, yet its biological roles these processes are unknown. Here, we generated zebrafish models deficient Ufm1 Ufl1 that exhibited telomere shortening associated with developmental delay, impaired hematopoiesis premature aging. We further report HeLa cells lacking UFL1 have instability of telomeres replicated by leading-strand synthesis. uncover MRE11 UFMylation necessary for the recruitment phosphatase PP1-α leading...
Rickettsia are intracellular bacteria typically associated with arthropods that can be transmitted to humans by infected vectors. spp. cause mild severe human disease a possible protection effect of corticosteroids when antibiotic treatments initiated. We identified laterally transferred toxin-antitoxin (TA) genetic elements, including vapB/C, in several genomes and showed they functional eukaryotic cells. also generated plaque assay monitor the formation lytic plaques over time demonstrated...
ABSTRACT We compared the fitness and lung pathogenicity of two isogenic clinical isolates Acinetobacter baumannii , one resistant (ABCR) other susceptible (ABCS) to colistin. In vitro ABCR exhibited slower growth kinetics than ABCS. a rat model pneumonia, was associated with less pronounced signs infection (lung bacterial count, systemic dissemination, damage) better outcome (ABCR ABCS mortality rates, 20 50%, respectively [ P = 0.03]).
Abstract Methanogens are acknowledged archaeal members of modern dental calculus microbiota and pathogen complexes. Their repertoire in ancient is poorly known. We therefore investigated archaea one hundred specimens collected from individuals recovered six archaeological sites France dated the 14 th to 19 centuries AD. Dental was demonstrated by macroscopic cone-beam observations. In 56 free PCR inhibition, sequencing identified Candidatus Methanobrevibacter sp. N13 44.6%, oralis 19.6%, a...
Coxiella burnetii is mainly transmitted by aerosols and responsible for multiple-organ lesions. Animal models have shown C. pathogenicity, but long-term outcomes still need to be clarified. We used a whole-body aerosol inhalation exposure system mimic the natural route of infection in immunocompetent (BALB/c) severe combined immunodeficient (SCID) mice. After an initial lung inoculum 10(4) cells/lung, outcome, serological response, hematological disorders, deep organ lesions were described...
Complex retrovirus genomes contain a variable number of accessory genes, among which is the vif gene. We investigated in vitro role gene caprine arthritis encephalitis virus (CAEV) by studying phenotype five mutants after infection primary goat synovial membrane (GSM) cells and blood-derived monocytes/macrophages. Any deletion introduced into resulted slow low viral replication production virions with an infectious titer lower than that wild-type particles. The could be restored trans...
There is evidence suggesting that actin binding to HIV-1 encoded proteins, or even dynamics themselves, might play a key role in virus budding and/or release from the infected cell. A crucial step reorganisation of cytoskeleton engagement various different GTP proteins. We have thus studied involvement GTP-binding proteins final steps viral replication cycle.Our results demonstrate production abolished when cellular involved polymerisation are inhibited with specific toxins.We propose new...
Abstract Whipple’s disease is a systemic infectious associated with the bacterium Tropheryma whipplei . Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies 1 lymph node biopsy from patients. showed presence of bacteria both CK20 + epithelial cells CD68 macrophages. Bacteria are found embedded biofilm hindering detection T. Only after...
A variant human immunodeficiency virus type 1 (HIV-1) vif gene, vifA45-2 , which encodes a protein lacking 19 amino acids at the C terminus but is fully functional in supporting HIV replication non-permissive cells has been described previously. By employing newly generated anti-VifA45 serum, further properties of VifA45 and its full-length counterpart, VifA45open, comparison to Vif from strain BH10 are reported permissive HeLa COS-7 cells. The results obtained using confocal microscopic...
Fanconi anemia (FA) is a genetic disorder associated with developmental defects, bone marrow failure and cancer. The FA pathway crucial for the repair of DNA interstrand crosslinks (ICLs). In this study, we have developed characterized new tool to investigate ICL repair: clickable version crosslinking agent melphalan which name click-melphalan. Our results demonstrate that click-melphalan as effective its unmodified counterpart in generating ICLs toxicity. lesions induced by can be detected...
Non-Hodgkin lymphoma has been linked to infection with Coxiella burnetii, potentially through overproduction of IL-10 during C. burnetii. Description a case report. We describe patient retroperitoneal non-Hodgkin and vascular Immunofluorescence staining fluorescence in situ hybridization targeting specific burnetii 16S rRNA were performed on the tissue sample obtained at diagnosis NHL. Both strongly positive for presence This provokes questions regarding potential association between NHL,...