A5049582405- Inflammasome and immune disorders
- Synthesis of heterocyclic compounds
- Synthesis and Characterization of Heterocyclic Compounds
- Erythrocyte Function and Pathophysiology
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Immune Cell Function and Interaction
- Heme Oxygenase-1 and Carbon Monoxide
- melanin and skin pigmentation
- Synthesis and biological activity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Neurobiology and Insect Physiology Research
- Calcium signaling and nucleotide metabolism
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Synthesis and Reactions of Organic Compounds
- Genetics and Neurodevelopmental Disorders
- PARP inhibition in cancer therapy
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Biochemical Analysis and Sensing Techniques
- CRISPR and Genetic Engineering
- Tryptophan and brain disorders
Instituto Murciano de Investigación Biosanitaria
2012-2025
Centro de Investigación Biomédica en Red
2020-2022
Centre for Biomedical Network Research on Rare Diseases
2020-2022
Instituto de Investigación de Enfermedades Raras
2021-2022
Instituto de Salud Carlos III
2021-2022
Universidad de Murcia
2008-2021
Hospital Universitario Virgen de la Arrixaca
2019
MRC Protein Phosphorylation and Ubiquitylation Unit
2014-2018
University of Dundee
2014-2018
Barcelona Biomedical Research Park
2009
Deubiquitylases (DUBs) are key regulators of the ubiquitin system which cleave moieties from proteins and polyubiquitin chains. Several DUBs have been implicated in various diseases attractive drug targets. We developed a sensitive fast assay to quantify vitro DUB enzyme activity using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Unlike other current assays, this method uses unmodified substrates, such as diubiquitin topoisomers. By analysing 42...
Abstract The expression of CD33, a restricted leukocyte antigen considered specific for myeloid lineage, has been studied extensively on lymphoid cells. We demonstrated that wide subsets mitogen- or alloantigen-activated human T and natural killer (NK) cells express CD33 at protein nucleic acid levels. CD33+ CD33– NK cell populations showed identical surface activation markers such as CD25, CD28, CD38, CD45RO, CD95. Myeloid cDNA were identical. However, had lower molecular weight, suggesting...
Several studies have revealed a correlation between chronic inflammation and nicotinamide adenine dinucleotide (NAD + ) metabolism, but the precise mechanism involved is unknown. Here, we report that genetic pharmacological inhibition of phosphoribosyltransferase (Nampt), rate-limiting enzyme in salvage pathway NAD biosynthesis, reduced oxidative stress, inflammation, keratinocyte DNA damage, hyperproliferation, cell death zebrafish models skin while all these effects were reversed by...
We have tested the usefulness of several commercial anti-CD33 monoclonal antibodies (mAb) to determine expression and localization two CD33 isoforms on hematopoietic cell lines. The isoform CD33m, a transmembrane splice variant lacking ligand-binding V immunoglobulin (Ig)-like domain, was detected by RT-polymerase chain reaction, western blot, confocal microscopy flow cytometry membrane human types. CD33m only mAb HIM3-4 surface, whereas WM53, P67.6, 4D3, HIM3-4, WM54, D3HL60.251 or MY9...
The kinase CK2 promotes the nuclear function of deubiquitylase OTUB1 in DNA damage repair.
The protein bcl‐xL is able to enhance the secretion of proinflammatory chemokine interleukin 8 (CXCL8) in human melanoma lines. In this study, we investigate whether bcl‐xL/CXCL8 axis important for promoting angiogenesis and aggressiveness vivo , using xenotransplantation assays zebrafish embryos. When injected into wild‐type embryos, bcl‐xL‐overexpressing cells showed enhanced dissemination angiogenic activity compared with control cells. Human CXCL8 elicited a strong proangiogenic embryos...
Article23 December 2014Open Access Source Data USP45 deubiquitylase controls ERCC1–XPF endonuclease-mediated DNA damage responses Ana B Perez-Oliva Corresponding Author MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University Dundee, UK Search for more papers by this author Christophe Lachaud Piotr Szyniarowski Ivan Muñoz Thomas Macartney Ian Hickson Janssen Research & Development, LLC, Spring House, PA, USA John Rouse Dario R Alessi Information 1, Lachaud1,...
Ubiquitin-fold modifier 1 (UFM1) is involved in neural and erythroid development, yet its biological roles these processes are unknown. Here, we generated zebrafish models deficient Ufm1 Ufl1 that exhibited telomere shortening associated with developmental delay, impaired hematopoiesis premature aging. We further report HeLa cells lacking UFL1 have instability of telomeres replicated by leading-strand synthesis. uncover MRE11 UFMylation necessary for the recruitment phosphatase PP1-α leading...
Abstract In 2020–2021, a “mysterious illness” struck Senegalese fishermen, causing severe acute dermatitis in over one thousand individuals following exposure through drift-net fishing activity. Here, by performing deep analysis of the environmental samples we reveal presence marine dinoflagellate Vulcanodinium rugosum and its associated cyclic imine toxins. Specifically, show that toxin PortimineA, strongly enriched samples, impedes ribosome function human keratinocytes, which subsequently...
The melanocortin 1 receptor, a Gs protein-coupled receptor expressed in epidermal melanocytes, is major determinant of skin pigmentation and phototype an important contributor to melanoma risk. MC1R activation stimulates synthesis black, strongly photoprotective eumelanin pigments. Several alleles are associated with red hair, fair skin, increased sensitivity ultraviolet radiation, cancer gene highly polymorphic, but only few naturally occurring have been functionally characterized, which...
Abstract Mucormycosis is a life-threatening fungal infection caused by various ubiquitous filamentous fungi of the Mucorales order, although Rhizopus spp. and Mucor are most prevalent causal agents. The limited therapeutic options available together with rapid progression difficult early diagnosis produce high mortality. Here, we developed an adult zebrafish model circinelloides which allowed us to confirm link between sporangiospore size virulence. Transcriptomic studies revealed local,...
The zona pellucida (ZP) is an extracellular glycoprotein matrix that surrounds all mammalian oocytes. Recent data have shown the presence of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) in ZP human rat rather than three proposed mouse model. In hamster (Mesocricetus auratus), it was previously described composed different glycoproteins, called ZP1, even though only ZP2 ZP3 been cloned thus far. aim study to determine whether might also express four, three, proteins. full-length cDNAs encoding...
Mahogunin ring finger-1 (MGRN1) is a RING domain-containing ubiquitin ligase mutated in mahoganoid, mouse mutation causing coat color darkening, congenital heart defects, high embryonic lethality, and spongiform neurodegeneration. The melanocortin hormones regulate pigmentation, cortisol production, food intake, body weight by signaling through five G protein-coupled receptors positively coupled to the cAMP pathway (MC1R-MC5R). Genetic analysis has shown that Mgrn1 an accessory protein for...
Abstract Background Macrophages take center stage in the tumor microenvironment, a niche composed of extracellular matrix and heterogeneous group cells, including immune ones. They can evolve during progression acquire Tumor-Associated Macrophage (TAMs) phenotype. The release cytokines by stromal influence secretion TAMs, which guarantee response to therapy. Among all factors able recruit polarize macrophages, we focused our attention on Bcl-xL, multifaceted member Bcl-2 family, whose...
Abstract Chronic inflammatory diseases are associated with hematopoietic lineage bias, including neutrophilia and anemia. We have recently identified that the canonical inflammasome mediates cleavage of master erythroid transcription factor GATA1 in stem progenitor cells (HSPCs). report here genetic inhibition Nlrp1 resulted reduced number neutrophils increased erythrocyte counts zebrafish larvae. also found NLRP1 human was inhibited by LRRFIP1 FLII, independently DPP9, both inhibitors...
The melanocortin 1 receptor (MC1R) is a G-protein-coupled (GPCR) crucial for the regulation of melanocyte proliferation and differentiation. MC1R activation by hormones triggers cAMP pathway stimulates extracellular-signal-regulated protein kinases ERK1 ERK2 to promote synthesis photoprotective eumelanin pigments, among other effects. Signaling from most GPCRs regulated β-arrestin (ARRB) family cytosolic multifunctional adaptor proteins, which mediate signal termination endocytosis...