A5018709867- Adenosine and Purinergic Signaling
- Inflammasome and immune disorders
- Neonatal Respiratory Health Research
- Toxin Mechanisms and Immunotoxins
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Cutaneous Melanoma Detection and Management
- Gout, Hyperuricemia, Uric Acid
- melanin and skin pigmentation
- Adolescent and Pediatric Healthcare
- Liver Disease and Transplantation
- Biochemical Analysis and Sensing Techniques
- S100 Proteins and Annexins
- Molecular Biology Techniques and Applications
- Meat and Animal Product Quality
- Antifungal resistance and susceptibility
- Paraoxonase enzyme and polymorphisms
- Organ Transplantation Techniques and Outcomes
- Identification and Quantification in Food
- Immune cells in cancer
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Bioactive Compounds and Antitumor Agents
- PARP inhibition in cancer therapy
- Neurological Complications and Syndromes
Biomedical Research Institute
2025
Universidad de Murcia
2014-2024
Instituto Murciano de Investigación Biosanitaria
2013-2022
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2016-2017
Hospital Universitario Virgen de la Arrixaca
2009-2016
Vall d'Hebron Institut de Recerca
2011
National Institutes of Health
2010
Zero to Three
2009
National Institutes of Health Clinical Center
2006
Universidad de Valladolid
1993-2002
Abstract Sepsis is characterized by a systemic inflammatory response followed immunosuppression of the host. Metabolic defects and mitochondrial failure are common in immunocompromised patients with sepsis. The NLRP3 inflammasome important for establishing an after activation purinergic P2X7 receptor. Here, we study cohort individuals intra-abdominal origin sepsis show that patient monocytes have impaired Furthermore, most sepsis-related deaths among whose profoundly altered. In from...
Arginine methylation of RAF proteins limits ERK activation after growth factor stimulation, thereby determining the biological response.
Abstract The activation of P2X7 receptor (P2X7R) on M1 polarized macrophages induces the assembly NLRP3 inflammasome leading to release pro-inflammatory cytokines and establishment inflammatory response. However, P2X7R signaling is uncoupled M2 without changes activation. In this study, we analyzed secretome in wild-type P2X7R-deficient either or proved that proteins released after stimulation goes beyond caspase-1 secretome. characterization P2X7R-secretome reveals a new function through...
Pyroptosis and intrinsic apoptosis are two forms of regulated cell death driven by active caspases where plasma membrane permeabilization is induced gasdermin pores. Caspase-1 induces D pore formation during pyroptosis, whereas caspase-3 promotes E apoptosis. These types accompanied mitochondrial outer due to BAK/BAX in the external mitochondria, some extent, this complex also affects inner facilitating DNA relocalization from matrix cytosol. However, detailed mechanism responsible for...
Abstract To assess markers of lung inflammation, we used SELDI‐TOF and 2‐DE to study changes in bronchoalveolar lavage (BAL) protein 33 subjects challenged with local bronchial endotoxin saline 11 patients acute respiratory distress syndrome (ARDS). Differences the spectra were assessed by t ‐test after baseline subtraction, normalization total ion current alignment m/z calibration. The temporal inflammatory BAL (6, 24 48 h following challenge) on hydrophobic binding chip surfaces revealed...
Tumor necrosis factor (TNF)-α is a major pro-inflammatory cytokine produced in response to toll-like receptor stimulation. TNF-α release controlled by the activity of converting enzyme (TACE) that cut membrane-bound shed its ectodomain as soluble cytokine. The purinergic P2X ligand-gated ion channel 7 (P2X7) activated elevated concentrations extracellular ATP and induces different pathways macrophages establish an inflammatory response. P2X7 promotes activation inflammasome interleukin-1β,...
The melanocortin 1 receptor, a Gs protein-coupled receptor expressed in epidermal melanocytes, is major determinant of skin pigmentation and phototype an important contributor to melanoma risk. MC1R activation stimulates synthesis black, strongly photoprotective eumelanin pigments. Several alleles are associated with red hair, fair skin, increased sensitivity ultraviolet radiation, cancer gene highly polymorphic, but only few naturally occurring have been functionally characterized, which...
P2X7 receptor activation induces the release of different cellular proteins, such as CD14, a glycosylphosphatidylinositol (GPI)-anchored protein to plasma membrane important for LPS signaling via TLR4. Circulating CD14 has been found at elevated levels in sepsis, but exact mechanism sepsis not established. Here, we show first time that extracellular vesicles, resulting net reduction macrophage functionally affects LPS, monophosphoryl lipid A, pro-inflammatory cytokine production. Also,...
Innate immunity plays a fundamental role in solid organ transplantation. Myeloid cells can sense danger signals or DAMPs released after tissue cell damage, such as during ischemia processes. This study aimed to identify cold storage of human liver and analyze their ability activate the inflammasome myeloid possible implications terms short-term outcomes transplantation.79 samples preservation solution (OPS) from 79 deceased donors were collected static storage. We used different analytical...
<title>Abstract</title> Pyroptosis and intrinsic apoptosis are two forms of regulated cell death driven by active caspases where plasma membrane permeabilization is induced gasdermin pores. Caspase-1 induces D pore formation during pyroptosis meanwhile caspase-3 promotes E apoptosis. These types accompanied mitochondrial outer due to BAK/BAX in the external mitochondria, some extent this complex also affects inner facilitating DNA relocalisation from matrix cytosol. However, detailed...
Sugar beet (Beta vulgaris L.) leaves contain virus-inducible type 1 (single chain) ribosome-inactivating proteins that have been named beetins. The structural and functional characterization, the cellular location, potential role of beetins as antiviral agents are reported here. Beetins formed a single polypeptide chain with varying degree glycosylation strongly inhibited in vitro protein synthesis rabbit reticulocyte lysates (IC50=1.15 ng ml−1) Vicia sativa L. cell-free system (IC50=68...
Approximately 20 % of obese dogs have metabolic disturbances similar to those observed in human syndrome, a condition known as obesity-related dysfunction. This is associated with insulin resistance and decreased circulating adiponectin concentrations, but clinical consequences not been reported. In order define better the changes dysfunction (ORMD), we compared plasma proteomes without ORMD. A proteomic analysis was conducted on samples from 8 male dogs, 4 ORMD The were first treated for...
The use of heat shock protein 90 (Hsp90) inhibitors is an attractive antineoplastic therapy. We wanted to compare the effects benzoquinone 17-allylamino-17-demethoxygeldanamycin (17-AAG, tanespimycin) and novel isoxazole resorcinol–based Hsp90 inhibitor NVP-AUY922 in a panel pancreatic colorectal carcinoma cell lines primary cultures derived from tumors excised patients. PANC-1, CFPAC-1, Caco-2 cells were intrinsically resistant 17-AAG but sensitive NVP-AUY922. Other cellular models both...
Abstract Axonal injury is a common feature of central nervous system insults that culminates with the death affected neurons, and an irreversible loss function. Inflammation important component neurodegenerative process, where microglia plays role by releasing proinflammatory factors as well clearing neurons phagocytosis. Here we have identified purinergic signaling through P2X7 receptor for neuronal in model optic nerve axotomy. We found deficient mice there delayed retinal ganglion cells...
Although saliva has esterase activity, this activity not been characterized or studied in individuals subjected to physical stress. The aim of report was develop and validate an automated spectrophotometric assay for total measurement human saliva, as well study the contribution different enzymes on its behaviour under stress healthy subjects. used 4-nitrophenyl acetate substrate precise, accurate provided low limits detection quantification. Inhibition with diisopropylfluorophosphate showed...
A high number of intratumoural infiltrating natural killer (NK) cells is associated with better survival in several types cancer, constituting an important first line defence against tumours. Hypoxia the core solid tumours induces cellular stress and ATP release into extracellular space where it triggers purinergic receptor activation on tumour-associated immune cells. The aim this study was to assess whether P2X7 by plays a role NK antitumour activity.We carried out vitro experiments using...
Abstract We hypothesized that invasive pulmonary aspergillosis (IPA) may generate a distinctive proteomic signature in plasma and bronchoalveolar lavage (BAL). Proteins BAL from two neutropenic rabbit models of IPA Pseudomonas pneumonia were analyzed by SELDI‐TOF MS. Hierarchical clustering analysis time course spectra demonstrated clusters peaks differentially regulated between (57 34 peaks, respectively, p <0.001). PCA proteins time‐dependent separation the infections. A random forest...
We explored the presence of germline alterations in CDK4 exon 2, CDKN2A and MC1R a hospital-based study 89 melanoma cases from families with at least two members affected by cutaneous melanoma. A total 30% kindreds studied were carriers variants, three these variants known predominant alleles that have been identified earlier Mediterranean populations (p.G101W, p.V59G c.358delG). observed higher frequency nonsynonymous Spanish (72%) respect to general population (60%). this kindred new...