A5025157938- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- S100 Proteins and Annexins
- Respiratory Support and Mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 and COVID-19 Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bacterial Identification and Susceptibility Testing
- Metabolomics and Mass Spectrometry Studies
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Hemodynamic Monitoring and Therapy
- Cardiac Arrest and Resuscitation
- Atomic and Subatomic Physics Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Proteomics Techniques and Applications
- Viral Infections and Outbreaks Research
- Thermal Regulation in Medicine
- Genomics and Phylogenetic Studies
- Disaster Response and Management
- Adrenal Hormones and Disorders
- Hemoglobinopathies and Related Disorders
- COVID-19 diagnosis using AI
- Long-Term Effects of COVID-19
National Institutes of Health Clinical Center
2016-2025
National Institutes of Health
2011-2025
National Heart Lung and Blood Institute
1989-2025
Government of the United States of America
2024
Harvard Pilgrim Health Care
2017
Harvard University
2017
Massachusetts General Hospital
2017
Center for Clinical Research (United States)
2008-2016
National Institute of Allergy and Infectious Diseases
1987-2016
National Institute of Neurological Disorders and Stroke
2016
Marked abnormalities in cardiovascular function accompany septic shock, and bacterial endotoxin is believed to be one of the principal mediators these abnormalities. To evaluate effects endotoxemia humans, we measured hemodynamic variables nine normal subjects given an intravenous bolus dose (Escherichia coli, 4 ng per kilogram body weight) six a saline, before three hours after administration. All then underwent volume loading with saline (mean, 2217 ml) during fourth fifth administration...
To evaluate the effect of endotoxin on fibrinolytic response, we administered Escherichia coli (4 ng per kilogram body weight) intravenously to 19 healthy volunteers and measured proteins, protease inhibitors, neutrophil elastase, von Willebrand factor in serial blood samples obtained over 24 hours. One hour after administration, level tissue plasminogen activator (t-PA) antigen rose from 10 23 milliliter, peaking at 52 milliliter three The alpha2-plasmin inhibitor–plasmin complexes...
Resistance to all first-line antibiotics necessitates the use of less effective or more toxic "reserve" agents. Gram-negative bloodstream infections (GNBSIs) harboring such difficult-to-treat resistance (DTR) may have higher mortality than phenotypes that allow for ≥1 active antibiotic.The Premier Database was analyzed inpatients with select GNBSIs. DTR defined as intermediate/resistant in vitro ß-lactam categories, including carbapenems and fluoroquinolones. Prevalence aminoglycoside...
Abstract Background Multiplex bead array assays permit simultaneous cytometric quantitation of multiple cytokines in solution by capturing these to spectrally distinct beads. Because several manufacturers offer reagents quantitate the same on a single instrument, comparison should be made determine whether kits yield similar data and are comparable enzyme‐linked immunosorbent assay (ELISA). Methods This study compared cytokine detection using Luminex 100. Twenty‐six serum samples from seven...
Although prednisone has been used to treat patients with idiopathic dilated cardiomyopathy, its efficacy not rigorously studied. We therefore randomly assigned 102 either treatment (60 mg per day) or a control group. At three months, improvement, defined prospectively as an increase in the ejection fraction of ≥ percentage points, was observed 53 percent receiving and 27 controls (P = 0.005). The mean (±SE) increased 4.3±1.5 points (from 17.9 ± 1.0 22.2 1.6 percent) group, compared 2.1 ±0.8...
BACKGROUND. Hepatitis C virus (HCV) infects approximately 170 million people worldwide and may lead to cirrhosis hepatocellular carcinoma in chronically infected individuals. Treatment is rapidly evolving from IFN-α–based therapies IFN-α–free regimens that consist of directly acting antiviral agents (DAAs), which demonstrate improved efficacy tolerability clinical trials. Virologic relapse after DAA therapy a common cause treatment failure; however, it not clear why occurs or whether certain...
Endotoxin, a lipopolysaccharide component of the outer membrane gram-negative bacteria, is involved in pathogenesis septic shock, but it unclear whether endotoxin alone capable causing all manifestations shock syndrome. In animals, causes many clinical features1 produces low-cardiac-output form that unlike hyperdynamic cardiovascular profile humans2,3. humans, administration (4 ng per kilogram body weight) triggers release cytokines,4 activates coagulation and fibrinolytic systems,5,6...
A new hypothesis with implications for the treatment of sepsis has recently been tested — that part pathophysiology is caused by unrestricted or inappropriate coagulation in microcirculation. Three agents block at different stages have now evaluated large, multicenter trials as adjunctive therapy sepsis. Neither antithrombin III nor tissue-factor-pathway inhibitor was effective patients severe septic shock.1,2 In contrast, activated protein C (drotrecogin alfa [activated]) associated a...
Objective To determine whether human endotoxemia is associated with a loss of the physiologic beat-to-beat variability heart rate. Design Prospective, randomized, crossover, single-blind study. Setting Clinical research center in federal, nonuniversity hospital. Subjects Healthy volunteers. Interventions Intravenous administration reference (Escherichia coli) endotoxin or saline placebo, without previous oral ibuprofen. Measurements and Main Results Electrocardiograms were continuously...
Section:ChooseTop of pageAbstract <<METHODS (see Online Data ...RESULTS ...DISCUSSIONReferencesCITING ARTICLES
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Recent studies demonstrate that HDL's ability to promote cholesterol efflux from macrophages associates strongly with cardioprotection in humans independently of HDL-cholesterol (HDL-C) and apoA-I, major protein. However, the mechanisms impair capacity during vascular disease are unclear. Inflammation, a well-established risk factor for cardiovascular disease, has been shown capacity. We therefore tested hypothesis impaired is mediated by specific changes its protein cargo. Humans acute...
During a 4.4-year period, nonspecific interstitial pneumonitis was seen in 41 of 110 (38%) patients with the acquired immunodeficiency syndrome and accounted for 32% (48/152) all episodes clinical pneumonitis. Diffuse alveolar damage typically feature pneumonitis, but neither lung biopsy nor bronchoalveolar lavage detected pathogen. Of these patients, 13 had no associated pulmonary tumor not been exposed to toxins, whereas 28 either concurrent Kaposi sarcoma, previous experimental therapies,...
In this study, we propose a novel pathological lung segmentation method that takes into account neighbor prior constraints and pathology recognition system. Our proposed framework has two stages; during stage one, adapted the fuzzy connectedness (FC) image algorithm to perform initial parenchyma extraction. parallel, estimate volume using rib-cage information without explicitly delineating lungs. This rudimentary, but intelligent estimation system allows comparison of differences between rib...
To assess determinants of prognosis for 43 patients with the acquired immunodeficiency syndrome (AIDS) and Pneumocystis carinii pneumonia, objective clinical histopathologic characteristics were analyzed acute long-term prognostic significance. Severe abnormalities on initial chest radiographs alveolar-arterial oxygen differences (AaPo2) greater than 30 mm Hg associated higher mortality during period treatment episode (p < 0.05). Decreased survival after diagnosis pneumonia correlated...
Sepsis is the focus of national quality improvement programs and a recent public reporting measure from Centers for Medicare Medicaid Services. However, diagnosing sepsis requires interpreting nonspecific signs can therefore be subjective. We sought to quantify interobserver variability in sepsis.We distributed five case vignettes patients with suspected or confirmed infection organ dysfunction sample practicing intensivists. Respondents classified cases as systemic inflammatory response...