A5025622241- Ethics and bioethics in healthcare
- Inflammasome and immune disorders
- Ethics in medical practice
- Health and Medical Education
- Social Sciences and Policies
- Biomedical Ethics and Regulation
- Adenosine and Purinergic Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- IL-33, ST2, and ILC Pathways
- Public Health and Environmental Issues
- Neuroethics, Human Enhancement, Biomedical Innovations
- Human Rights and Immigration
- Sports injuries and prevention
- Poverty, Education, and Child Welfare
- Ethics in Clinical Research
- Law, Ethics, and AI Impact
- Disaster Response and Management
- Child and Adolescent Health
- Tendon Structure and Treatment
- Public Health in Brazil
- Comparative International Legal Studies
- Labor Law and Work Dynamics
- Immigration and Intercultural Education
- Education in Rural Contexts
- Business, Innovation, and Economy
Universidad del Rosario
2003-2024
Instituto Murciano de Investigación Biosanitaria
2014-2024
Universidad El Bosque
2018-2023
Sociedad Española de Nefrología
2022
Departamento de Salud
2009-2020
Hospital Regional Universitario de Málaga
2019
Instituto de Investigación Biomédica de Málaga
2019
Pontificia Universidad Javeriana
2010-2018
Centro de Investigación Biomédica en Red
2012-2016
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2012-2016
Interleukin-1β (IL-1β) is a critical regulator of the inflammatory response. IL-1β not secreted through conventional ER–Golgi route protein secretion, and to date its mechanism release has been unknown. Crucially, secretion depends upon processing precursor form following activation multimolecular inflammasome complex. Using novel reversible pharmacological inhibitor process, in combination with biochemical, biophysical, real-time single-cell confocal microscopy macrophage cells expressing...
Prostaglandins (PGs) are important lipid mediators involved in the development of inflammatory associated pain and fever. PGE2 is a well-established endogenous pyrogen activated by proinflammatory cytokine interleukin (IL)-1β. P2X7 receptors (P2X7Rs) expressed cells stimulated danger signal extracellular ATP to activate inflammasome release IL-1β. Here we show that P2X7R activation required for other autacoids independent activation, with an EC50 IL-1β 1.58 1.23 mM, respectively....
Abstract The activation of P2X7 receptor (P2X7R) on M1 polarized macrophages induces the assembly NLRP3 inflammasome leading to release pro-inflammatory cytokines and establishment inflammatory response. However, P2X7R signaling is uncoupled M2 without changes activation. In this study, we analyzed secretome in wild-type P2X7R-deficient either or proved that proteins released after stimulation goes beyond caspase-1 secretome. characterization P2X7R-secretome reveals a new function through...
Abstract Apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC) is key adaptor molecule required for the inflammatory processes. ASC acts by bridging NLRP proteins, such as NLRP3, with procaspase-1 within inflammasome complex, which subsequently results in activation of caspase-1 and secretion IL-1β IL-18. In response to bacterial infection, also forms specks self-oligomerization activate induce pyroptosis. Hitherto, role these NLRP3 danger signals,...
Pyroptosis and intrinsic apoptosis are two forms of regulated cell death driven by active caspases where plasma membrane permeabilization is induced gasdermin pores. Caspase-1 induces D pore formation during pyroptosis, whereas caspase-3 promotes E apoptosis. These types accompanied mitochondrial outer due to BAK/BAX in the external mitochondria, some extent, this complex also affects inner facilitating DNA relocalization from matrix cytosol. However, detailed mechanism responsible for...
Tumor necrosis factor (TNF)-α is a major pro-inflammatory cytokine produced in response to toll-like receptor stimulation. TNF-α release controlled by the activity of converting enzyme (TACE) that cut membrane-bound shed its ectodomain as soluble cytokine. The purinergic P2X ligand-gated ion channel 7 (P2X7) activated elevated concentrations extracellular ATP and induces different pathways macrophages establish an inflammatory response. P2X7 promotes activation inflammasome interleukin-1β,...
The NLRP3 inflammasome coordinates inflammation in response to different pathogen- and damage-associated molecular patterns, being implicated infectious, chronic inflammatory, metabolic degenerative diseases. In tendinopathic lesions, non-resolving mechanisms produce a condition that impairs tissue healing which therefore complicates their clinical management. Percutaneous needle electrolysis consists of the application galvanic current is an emerging treatment for tendinopathies. present...
Signaling through the inflammasome is important for inflammatory response. Low concentrations of intracellular K+ are associated with specific oligomerization and activation NLRP3 inflammasome, a type involved in sterile inflammation. After oligomerization, ASC protein binds forms oligomeric filaments that culminate large complexes named specks. specks also initiated from different scaffolds, such as AIM2, NLRC4, or Pyrin. oligomers recruit caspase-1 then induce its interactions between...
The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations UTP ATP applied macrophage priming enhance IL-1β production when subsequently NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency pharmacological inhibition purinergic receptor P2Y2 reverted increase induced by nucleotides. was found dependent expression Il1b...
The HIV-1 gp41 envelope glycoprotein is responsible for the membrane fusion between virus and target cell. According to recent models, N-terminal coiled-coil (NHR) region of involved in forming interfaces neighboring helices six-helix bundle, as well binding perturbation. In order get new insights into viral mechanism, two peptides, pFP15 pFP23, pertaining first part NHR domain were studied regarding their structure ability induce leakage, aggregation, fusion, affinity toward specific...
NLRP3 inflammasome is a multiprotein complex responsible for the activation of inflammatory caspase-1, resulting in processing and release pro-inflammatory cytoquines IL-1β IL-18 (Schroder Tschopp, 2010). This composed sensor protein connected to caspase-1 through adaptor ASC (apoptosis-associated speck-like with caspase-recruitment domain) We others have reported that upon functional oligomeric particles were released from cells, acting as danger signals amplify inflammation by promoting...
Propósito/Contexto. La pandemia por COVID-19 y la emergencia sanitaria desencadenada permite analizar, desde bioética el bioderecho, los problemas relativos al principio de justicia distributiva, en contexto estructural inequidad endémica Colombia.Metodología/Enfoque. Este artículo realiza un análisis ajustes normativos Colombia relación con garantía del derecho a salud, atención SARS-CoV-2. Resultados/Hallazgos. A pesar declaración estado Colombia, persistencia social económica distintas...
La integridad académica es uno de los propósitos principales la educación alta calidad. No obstante, el fenómeno deshonestidad un problema creciente a nivel global que se ha sofisticado por acceso las nuevas tecnologías información y comunicación. Este trabajo aborda en contexto enseñanza medicina, sus causas, modalidades, factores predisponentes estrategias prevención, detección sanción. multicausal confluyen relativos al entorno cultura moral, personales, currículo oculto, políticas...
Muchos ninos trabajan en las calles de Bogota. La cantidad exacta se desconoce y es muy poca la informacion disponible acerca este grupo. El presente estudio identifico el comportamiento variables edad, genero, desplazamiento por violencia, escolaridad, tipo actividad desarrollada, tiempo permanencia calle cobertura seguridad social, dentro un grupo 162 bogotanos con actividades economicas calle. Metodologia: realizo descriptivo que entrevistaron los encontraban trabajando ciertas ciudad...
Although nonopiate analgesics may be particularly useful in the immediate postoperative period after major surgery, their use has been associated with haemodynamic adverse effects during pain treatment and critically ill patients intensive care. The effect of a single intravenous dose metamizol (dipyrone) 2 g, ketorolac 30 mg propacetamol 1 g on variables control heart surgery is compared. Seventy-two undergoing elective coronary and/or valve were included cohort study 1-years duration...
<p>La relación de los profesionales la salud con pacientes tiene como piedra angular el respeto del derecho las personas a autodeterminarse, tomar decisiones libres e informadas respecto su vida, y cuerpo. El final vida es uno más importantes momentos vitales en cual definitivo garantizar que paciente pueda una serie sobre múltiples aspectos, son deseo o no ser informado, aceptar medidas terapéuticas cuidado paliativo, rechazar aquellas fútiles, definir quién puede acceder sus datos...