A5028225139- Vestibular and auditory disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Inflammasome and immune disorders
- Olfactory and Sensory Function Studies
- Glaucoma and retinal disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ocular Surface and Contact Lens
- Ophthalmology and Eye Disorders
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Visual perception and processing mechanisms
- Myofascial pain diagnosis and treatment
- Sphingolipid Metabolism and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Streptococcal Infections and Treatments
- Neuroscience of respiration and sleep
- Kawasaki Disease and Coronary Complications
- Tryptophan and brain disorders
- Cerebral Venous Sinus Thrombosis
- Photoreceptor and optogenetics research
- Long-Term Effects of COVID-19
- Musculoskeletal pain and rehabilitation
- Laser Applications in Dentistry and Medicine
- Marine Invertebrate Physiology and Ecology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Institut de Pharmacologie et de Biologie Structurale
2021-2025
Université de Toulouse
2021-2023
Université Toulouse III - Paul Sabatier
2021-2023
Centre National de la Recherche Scientifique
2017-2023
Laboratoire de Neurosciences Cognitives
2019-2021
Aix-Marseille Université
2017-2021
Laboratoire de Neurosciences Intégratives et Adaptatives
2017
Institut Gustave Roussy
2003
Abstract Inflammation is a complex physiological process triggered in response to harmful stimuli 1 . It involves cells of the immune system capable clearing sources injury and damaged tissues. Excessive inflammation can occur as result infection hallmark several diseases 2–4 The molecular bases underlying inflammatory responses are not fully understood. Here we show that cell surface glycoprotein CD44, which marks acquisition distinct phenotypes context development, immunity cancer...
Multiple regulated neutrophil cell death programs contribute to host defense against infections. However, despite expressing all necessary inflammasome components, neutrophils are thought be generally defective in Caspase-1-dependent pyroptosis. By screening different bacterial species, we found that several Pseudomonas aeruginosa ( P . ) strains trigger pyroptosis human and murine neutrophils. Notably, deletion of Exotoxins U or S enhanced pyroptosis, suggesting these exotoxins interfere...
Human airway and corneal epithelial cells, which are critically altered during chronic infections mediated by Pseudomonas aeruginosa, specifically express the inflammasome sensor NLRP1. Here, together with a companion study, we report that NLRP1 detects exotoxin A (EXOA), ribotoxin released P. aeruginosa type 2 secretion system (T2SS), infection. Mechanistically, EXOA-driven eukaryotic elongation factor (EEF2) ribosylation covalent inactivation promote ribotoxic stress subsequent activation,...
The coronavirus disease 2019 (COVID-19) pandemic continues to cause significant morbidity and mortality worldwide. Since a large portion of the world's population is currently unvaccinated or incompletely vaccinated has limited access approved treatments against COVID-19, there an urgent need continue research on treatment options, especially those at low cost which are immediately available patients, particularly in low- middle-income countries. Prior vitro observational studies have shown...
Abstract In 2020–2021, a “mysterious illness” struck Senegalese fishermen, causing severe acute dermatitis in over one thousand individuals following exposure through drift-net fishing activity. Here, by performing deep analysis of the environmental samples we reveal presence marine dinoflagellate Vulcanodinium rugosum and its associated cyclic imine toxins. Specifically, show that toxin PortimineA, strongly enriched samples, impedes ribosome function human keratinocytes, which subsequently...
Vestibular pathologies are difficult to diagnose. Existing devices make it possible quantify and follow the evolution of posturo-locomotor symptoms following vestibular loss in static conditions. However, today, there no diagnostic tools allowing quantitative spontaneous analysis these dynamic situations. With this mind, we used an open-field video tracking test aiming at identifying specific markers a rodent model pathology. Using Ethovision XT 14 software (Noldus), identified quantified...
ABSTRACT Damage to cochlear primary afferent synapses has been shown be a key factor in various auditory pathologies. Similarly, the selective lesioning of vestibular might an underlying cause peripheral vestibulopathies that vertigo and dizziness, for which pathophysiology is currently unknown. To thoroughly address this possibility, we selectively damaged synaptic contacts between hair cells neurons mice through transtympanic administration glutamate receptor agonist. Using combination...
Abstract Objective Unilateral labyrinthectomy (UL) and unilateral vestibular neurectomy (UVN) are two surgical methods to produce lesions in the mouse. The objective of this study was describe technique both methods, compare functional compensation using vestibulo-ocular reflex-based tests. Methods UL UVN were each performed on groups seven ten mice, respectively. Main landmarks facial nerve, external auditory canal sternomastoid digastric muscles. For UL, muscle elevated expose mastoid,...
Unilateral vestibular lesions induce a syndrome, which recovers over time due to compensation. The therapeutic effect of L-Thyroxine (L-T4) on compensation was investigated by behavioral testing and immunohistochemical analysis in rat model unilateral neurectomy (UVN). We demonstrated that short-term L-T4 treatment reduced the syndrome significantly promoted Thyroid hormone receptors (TRα TRβ) type II iodothyronine deiodinase (DIO2) were present nuclei (VN), supporting local action L-T4....
The vestibular system plays a crucial role in maintaining postural balance. Unilateral lesions result typical syndrome characterized by imbalance, altered locomotor patterns and gaze stabilization, as well cognitive neurovegetative disorders. One of the main difficulties encountered development new anti-vertigo drugs is lack sensitivity evaluation this syndrome. Qualitative assessments have been developed, but methods conducting quantitative evaluations are critically lacking. Recently, with...
Acute peripheral vestibulopathy leads to a cascade of symptoms involving balance and gait disorders that are particularly disabling for vestibular patients. Vestibular rehabilitation protocols have proven be effective in improving compensation clinical practice. Yet, the underlying neurobiological correlates remain unknown. The aim this study was highlight behavioural cellular consequences protocol adapted rat model unilateral neurectomy. We developed progressive sensory-motor task, were...
Due to their anti-inflammatory action, corticosteroids are the reference treatment for brain injuries and many inflammatory diseases. However, benefits of acute corticotherapy now being questioned, particularly in case peripheral vestibulopathies (APV), characterized by a vestibular syndrome composed sustained spinning vertigo, spontaneous ocular nystagmus oscillopsia, perceptual-cognitive, posturo-locomotor, vegetative disorders. We assessed effectiveness corticotherapy, functional role...
Vestibular disorders, by inducing significant posturo-locomotor and cognitive can significantly impair the most basic tasks of everyday life. Their precise diagnosis is essential to implement appropriate therapeutic countermeasures. Monitoring their evolution also very important validate or, on contrary, adapt undertaken actions. To date, methods impairments are restricted examinations that often lack sensitivity precision. In present work we studied alterations dynamic weight distribution...
Regulated cell necrosis supports immune and anti-infectious strategies of the body; however, dysregulation these processes drives pathological organ damage. Pseudomonas aeruginosa expresses a phospholipase, ExoU that triggers host through poorly characterized pathway. Here, we investigated molecular cellular mechanisms ExoU-mediated necrosis. We show peroxidised phospholipids enhance phospholipase activity, which non-immune cells. Conversely, both endogenous lipid peroxidation regulator GPX4...
Unilateral vestibular loss (UVL) induces a syndrome composed of posturo-locomotor, oculomotor, vegetative, and perceptivo-cognitive symptoms. With time, these functional deficits progressively disappear due to phenomenon called compensation, known be supported by the expression in deafferented nuclei (VNs) various adaptative plasticity mechanisms. UVL is induce neuroinflammatory response within VNs, thought caused structural alteration primary afferents. The acute inflammatory response,...
We have previously reported in a feline model of acute peripheral vestibulopathy (APV) that the sudden, unilateral, and irreversible loss vestibular inputs induces selective overexpression small conductance calcium-activated potassium (SK) channels brain stem nuclei. Pharmacological blockade these ion by antagonist apamin significantly alleviated evoked syndrome accelerated compensation. In this follow-up study, we aimed at testing, using behavioral approach, whether antivertigo (AV) effect...
ABSTRACT The intracellular inflammasome complex have been implicated in the maladaptive tissue damage and inflammation observed chronic Pseudomonas aeruginosa infection. Human airway corneal epithelial cells, which are critically altered during infections mediated by P. , specifically express sensor NLRP1. Here, together with a companion study, we report that NLRP1 detects Exotoxin A (EXOA), ribotoxin released Type 2 Secretion System (T2SS) Mechanistically, EXOA-driven Eukaryotic Elongation...
Inflammation observed in SARS-CoV-2-infected patients suggests that inflammasomes, pro-inflammatory intracellular complexes, regulate various steps of infection. Lung epithelial cells express inflammasome-forming sensors and constitute the primary entry door SARS-CoV-2. Here, we describe NLRP1 inflammasome detects SARS-CoV-2 infection human lung cells. Specifically, is cleaved at Q333 site by multiple coronavirus 3CL proteases, which triggers assembly, cell death limits production infectious...