A5045269326- Micro and Nano Robotics
- Lipid Membrane Structure and Behavior
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Malaria Research and Control
- Nonlinear Dynamics and Pattern Formation
- Genetic and Kidney Cyst Diseases
- Material Dynamics and Properties
- Advanced Thermodynamics and Statistical Mechanics
- Orbital Angular Momentum in Optics
- Pickering emulsions and particle stabilization
- Force Microscopy Techniques and Applications
- Surfactants and Colloidal Systems
- Spectroscopy and Quantum Chemical Studies
- Nanopore and Nanochannel Transport Studies
- Cell Image Analysis Techniques
- DNA and Nucleic Acid Chemistry
- Mosquito-borne diseases and control
- Electrostatics and Colloid Interactions
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Protein Structure and Dynamics
University of Cambridge
2016-2025
Human Genetic of Infectious Diseases
2022
Université Paris Cité
2022
Cavendish Hospital
2015-2022
University of Bath
2022
Ifakara Health Institute
2022
IFOM
2020
Imperial College London
2018
University of Copenhagen
2018
University of Milan
2000-2017
Pathogen recognition by nucleotide-binding oligomerization domain-like receptor (NLR) results in the formation of a macromolecular protein complex (inflammasome) that drives protective inflammatory responses host. It is thought number inflammasome complexes forming cell determined NLRs being activated, with each NLR initiating its own assembly independent one another; however, we show here important foodborne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) simultaneously...
Audio signals generated by the human body (e.g., sighs, breathing, heart, digestion, vibration sounds) have routinely been used clinicians as indicators to diagnose disease or assess progression. Until recently, such were usually collected through manual auscultation at scheduled visits. Research has now started use digital technology gather bodily sounds from stethoscopes) for cardiovascular respiratory examination, which could then be automatic analysis. Some initial work shows promise in...
Abstract To identify Coronavirus disease (COVID-19) cases efficiently, affordably, and at scale, recent work has shown how audio (including cough, breathing voice) based approaches can be used for testing. However, there is a lack of exploration biases methodological decisions impact these tools’ performance in practice. In this paper, we explore the realistic audio-based digital testing COVID-19. investigate this, collected large crowdsourced respiratory dataset through mobile app,...
Two colloidal spheres are maintained in oscillation by switching the position of an optical trap when a sphere reaches limit position, leading to oscillations that bounded amplitude but free phase and period. The interaction between oscillators is only through hydrodynamic flow induced their motion. We prove absence stochastic noise antiphase dynamical state stable, we show how period depends on coupling strength. Both features observed experimentally. As natural frequencies made...
Swimming and pumping at low Reynolds numbers are subject to the "Scallop theorem", which states that there is no net fluid flow for time-reversible motions. Microscale organisms such as bacteria cells this constraint, so existing future artificial "nano-bots" or microfluidic pumps. We study a very simple mechanism induce pumping, based on forced motion of three colloidal beads through cycle breaks time-reversal symmetry. Optical tweezers used vary inter-bead distance. This model inspired by...
TLR4 signalling through the MyD88 and TRIF-dependent pathways initiates translocation of transcription factor NF-κB into nucleus. In cell population studies using mathematical modeling functional analyses, Cheng et al. suggested that LPS-driven activation MyD88, in absence TRIF, impairs translocation. We tested model proposed by real-time single analysis macrophages expressing EGFP-tagged p65 a TNFα promoter-driven mCherry. Following LPS stimulation, cells lacking TRIF show pattern dynamics...
Optical microscopes are an essential tool for both the detection of disease in clinics, and scientific analysis. However, much world access to high-performance microscopy is limited by upfront cost maintenance equipment. Here we present open-source, 3D-printed, fully-automated laboratory microscope, with motorised sample positioning focus control. The microscope highly customisable, a number options readily available including trans- epi- illumination, polarisation contrast imaging,...
We designed a microfluidic chemostat consisting of 600 sub-micron trapping/growth channels connected to two feeding channels. The microchemostat traps E. coli cells and forces them grow in lines for over 50 generations. Excess cells, including the mother captured at start process, are removed from both ends growth by media flow. With aid time-lapse microscopy, we have monitored dynamic properties such as rate GFP expression single-cell level many generations while maintaining population...
Mechanical forces are obviously important in the assembly of three-dimensional multicellular structures, but their detailed role is often unclear. We have used growing microcolonies bacterium Escherichia coli to investigate mechanical transition from two-dimensional growth (on interface between a hard surface and soft agarose pad) (invasion agarose). measure position within colony where invasion happens, cell density size at as functions concentration agarose. use phenomenological theory,...
Infection and tissue damage induces assembly of supramolecular organizing centres (SMOCs)), such as the Toll-like receptor (TLR) MyDDosome, to co-ordinate inflammatory signaling. SMOC is thought drive digital all-or-none responses, yet TLR activation by diverse microbes anything from mild severe inflammation. Using single-molecule imaging TLR4-MyDDosome signaling in living macrophages, we find that MyDDosomes assemble within minutes TLR4 stimulation. TLR4/MD2 leads only formation...
Elastic capsules, prepared from droplets or bubbles attached to a capillary (as in pendant drop tensiometer), can be deflated by suction through the capillary. We study this deflation and show that combined analysis of shape wrinkling characteristics enables us determine elastic properties situ. Shape contours are analyzed fitted using equations derived nonlinear membrane-shell theory give modulus, Poisson ratio stress distribution membrane. include wrinkles, which generically form upon...
Short DNA linkers are increasingly being exploited for driving specific self-assembly of Brownian objects. DNA-functionalised colloids can assemble into ordered or amorphous materials with tailored morphology. Recently, the same approach has been applied to compliant units, including emulsion droplets and lipid vesicles. The liquid structure these substrates introduces new degrees freedom: tethers diffuse rearrange, radically changing physics interactions. Unlike droplets, vesicles extremely...
The mean size of exponentially dividing E. coli cells cultured in different nutrient conditions is known to depend on the growth rate only. However, joint fluctuations relating cell size, doubling time and individual are only starting be characterized. Recent studies bacteria (i) revealed near constancy extension a single cycle (adder mechanism), (ii) reported universal trend where spread both times linear function population means these variables. Here, we combine experiments theory use...
The type 2 cytokine-high asthma endotype (T2H) is characterized by IL-13-driven mucus obstruction of the airways. To further investigate this incompletely understood pathobiology, we characterize IL-13 effects on human airway epithelial cell cultures using single-cell RNA sequencing, finding that generates a distinctive transcriptional state for each type. Specifically, discover secretory program induced in all types which converts both and defense cells into metaplastic with emergent mucin...
Salmonellosis is one of the leading causes food poisoning worldwide. Controlling bacterial burden essential to surviving infection. Nucleotide-binding oligomerization domain-like receptors (NLRs), such as NLRC4, induce inflammasome effector functions and play a crucial role in controlling Salmonella Inflammasome-dependent production IL-1β recruits additional immune cells site infection, whereas inflammasome-mediated pyroptosis macrophages releases bacteria for uptake by neutrophils. Neither...
The development of fast and accurate screening tools, which could facilitate testing prevent more costly clinical tests, is key to the current pandemic COVID-19. In this context, some initial work shows promise in detecting diagnostic signals COVID-19 from audio sounds. paper, we propose a voice-based framework automatically detect individuals who have tested positive for We evaluate performance proposed on subset data crowdsourced our app, containing 828 samples 343 participants. By...