A5043934355- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- Biocrusts and Microbial Ecology
- Force Microscopy Techniques and Applications
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Electrostatics and Colloid Interactions
- Slime Mold and Myxomycetes Research
- Microfluidic and Bio-sensing Technologies
- Cellular Mechanics and Interactions
- Plant and Biological Electrophysiology Studies
- Sphingolipid Metabolism and Signaling
- Algal biology and biofuel production
- Characterization and Applications of Magnetic Nanoparticles
- Microfluidic and Capillary Electrophoresis Applications
- Surfactants and Colloidal Systems
- Electrochemical Analysis and Applications
- Advanced Materials and Mechanics
- Glycosylation and Glycoproteins Research
- Advanced Fluorescence Microscopy Techniques
- Tissue Engineering and Regenerative Medicine
- Mechanical and Optical Resonators
- Micro and Nano Robotics
Lehigh University
2017-2024
University of Cambridge
2012-2022
University of Washington
2008-2013
University of Oregon
2005
The viscosity of lipid bilayer membranes plays an important role in determining the diffusion constant embedded proteins and dynamics membrane deformations, yet it has historically proven very difficult to measure. Here we introduce a new method based on quantification large-scale circulation patterns induced inside vesicles adhered solid surface subjected simple shear flow microfluidic device. Particle image velocimetry spinning disk confocal imaging tracer particles outside vesicle...
Near a critical point, the time scale of thermally induced fluctuations diverges in manner determined by dynamic universality class. Experiments have verified predicted three-dimensional exponents many systems, but similar experiments two dimensions been lacking for case conserved order parameter. Here we analyze time-dependent correlation functions quasi-two-dimensional lipid bilayer water to show that its dynamics agree with recently In particular, effective exponent z(eff) crosses over...
Deformations of cell sheets are ubiquitous in early animal development, often arising from a complex and poorly understood interplay shape changes, division, migration. Here, we explore perhaps the simplest example sheet folding: “inversion” process algal genus Volvox, during which spherical embryos turn themselves inside out through hypothesized to arise changes alone. We use light microscopy obtain first three-dimensional visualizations inversion vivo, develop theory this process, appear...
The prevalence of multicellular organisms is due in part to their ability form complex structures. How cells pack these structures a fundamental biophysical issue, underlying functional properties. However, much remains unknown about how cell packing geometries arise, and they are affected by random noise during growth - especially absent developmental programs. Here, we quantify the statistics cellular neighborhoods two different eukaryotes: lab-evolved 'snowflake' yeast green alga
Here we use nuclear magnetic resonance to measure the solubility limit of several biologically relevant sterols in electroformed giant unilamellar vesicle membranes containing phosphatidylcholine (PC) lipids ratios 1 : X DOPC DPPC sterol. We find limits cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol be 65–70 mol%, ∼35 30–35 20–25 ∼40 respectively. The low solubilities stigmasterol β-sitosterol, which differ from cholesterol only their alkyl tails, show that subtle...
When lipid membranes curve or are subjected to strong shear forces, the two apposed leaflets of bilayer slide past each other. The drag that one leaflet creates on other is quantified by coefficient interleaflet friction, b. Existing measurements this range over several orders magnitude, so we used a recently developed microfluidic technique measure it systematically in supported membranes. Fluid stress was force top membrane stationary lower leaflet. Here, show yields reproducible...
Variability is emerging as an integral part of development. It therefore imperative to ask how access the information contained in this variability. Yet most studies development average their observations and, discarding variability, seek derive models, biological or physical, that explain these observations. Here, we analyse variability a study cell sheet folding green alga Volvox, whose spherical embryos turn themselves inside out process sharing invagination, expansion, involution, and...
Glypican-1 and its heparan sulfate (HS) chains play important roles in modulating many biological processes including growth factor signaling. is bound to a membrane surface via glycosylphosphatidylinositol (GPI)-anchor. In this study, we used all-atom molecular modeling simulation explore the structure, dynamics, interactions of GPI-anchored glypican-1, three HS chains, membranes, ions. The folded glypican-1 core structure stable, but has substantial degrees freedom terms movement...
Fluid flow near biological membranes influences cell functions such as development, motility, and environmental sensing. Flow can laterally transport extracellular membrane proteins located at the cell-fluid interface. To determine whether this contributes to signaling in cells, quantitative knowledge of forces acting on is required. Here, we demonstrate a method for measuring flow-mediated lateral lipid-anchored proteins. We rupture giant unilamellar vesicles form discrete patches supported...