A5072872290- Sperm and Testicular Function
- Glycosylation and Glycoproteins Research
- Reproductive Biology and Fertility
- Neuropeptides and Animal Physiology
- Cellular transport and secretion
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Receptor Mechanisms and Signaling
- Hypothalamic control of reproductive hormones
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Endoplasmic Reticulum Stress and Disease
- Turtle Biology and Conservation
- Erythrocyte Function and Pathophysiology
- Animal Genetics and Reproduction
- Caveolin-1 and cellular processes
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- Aquaculture disease management and microbiota
- Proteoglycans and glycosaminoglycans research
- S100 Proteins and Annexins
- Reproductive Physiology in Livestock
- Reproductive biology and impacts on aquatic species
- Veterinary Equine Medical Research
- Biomedical Research and Pathophysiology
- Veterinary Medicine and Surgery
Universidad de Murcia
2006-2024
Instituto Murciano de Investigación Biosanitaria
2021-2024
Universidad de Las Palmas de Gran Canaria
2010
Municipal Institute for Medical Research
2009
Barcelona Biomedical Research Park
2009
Universitat de Barcelona
2000
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2000
University of Geneva
2000
University of Freiburg
2000
Hammersmith Hospital
1984-1988
Actin is involved in the organization of Golgi complex and Golgi-to-ER protein transport mammalian cells. Little, however, known about regulation Golgi-associated actin cytoskeleton. We provide evidence that Cdc42, a small GTPase regulates dynamics, controls transport. located GFP-Cdc42 lateral portions cisternae COPI-coated noncoated intermediates. Overexpression Cdc42 its activated form Cdc42V12 inhibited retrograde Shiga toxin from to ER, redistribution KDEL receptor, ER accumulation...
The distributions of three novel peptides, 7B2, neuromedin B, and U, in rat, mouse, human pituitaries, rat hypothalamus, 30 pituitary tumors were investigated with immunocytochemistry. Immunoreactivity for 7B2 was present gonadotropes, intermediate lobe cells posterior nerve fibers rats mice, hypothalamus (particularly the median eminence), eight gonadotropinomas. In gonadectomized rats, larger, more numerous LHβ- 7B2-immunoreactive gonadotropes seen than controls. Extractable 7B2-like...
It has been shown previously that the morphology and subcellular positioning of Golgi complex is controlled by actin microfilaments. To further characterize association between microfilaments complex, we have used Clostridium botulinum toxins C2 C3, which specifically inhibit polymerization cause depolymerization F-actin in intact cells ADP ribosylation G-actin monomers Rho small GTP-binding protein, respectively. Normal rat kidney treated with showed disruption collapse occurred...
Abstract Sperm binding activity has been detected in zona pellucida (ZP) glycoproteins and it is generally accepted that this resides the carbohydrate moieties. In present study we aim to identify some of specific molecules involved bovine sperm–ZP interaction. We performed sperm competition assays, vitro fecundation (IVF) combination with different lectins, antibodies neuraminidase digestion, chemical cytochemical analysis ZP. Both MAA lectin recognising α‐2,3‐linked sialic acid from...
The zona pellucida (ZP) is an extracellular glycoprotein matrix that surrounds all mammalian oocytes. Recent data have shown the presence of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) in ZP human rat rather than three proposed mouse model. In hamster (Mesocricetus auratus), it was previously described composed different glycoproteins, called ZP1, even though only ZP2 ZP3 been cloned thus far. aim study to determine whether might also express four, three, proteins. full-length cDNAs encoding...
The MAL proteolipid, an integral protein present in glycolipid- and cholesterol-enriched membrane (GEM) rafts, is element of the machinery necessary for apical sorting polarized epithelial Madin-Darby canine kidney cells. was first member identified extended family proteins that have significant overall sequence identity. In this study we used a newly generated monoclonal antibody to investigate unedited family, named BENE, which found be expressed endothelial-like ECV304 cells normal human...
Abstract Background : The zona pellucida (ZP), an extracellular matrix which surrounds mammalian oocytes, is formed by different glycoproteins. Several studies have revealed that carbohydrate residues present in glycoproteins of ZP play a key role the sperm‐egg recognition. However, origin and biochemical composition remain to be completely resolved. Methods from rat ovarian follicles were investigated at light electron microscopic level application lectins conjugated peroxidase,...
Immunofluorescence and cryoimmunoelectron microscopy were used to examine the morphologic functional effects on Golgi complex when protein transport is blocked at ERGIC (endoplasmic reticulum‐Golgi intermediate compartment) in HeLa cells incubated low temperature (15 °C). At this temperature, showed long tubules containing resident glycosylation enzymes but not matrix proteins. These Golgi‐derived also lacked anterograde (VSV‐G) or retrograde (Shiga toxin) cargo. The formation of was...
The glycoproteins of the intestinal mucosa five vertebrate species including fishes (Sparus auratus), amphibians (Rana perezi), reptiles (Testudo graeca), birds (Gallus gallus) and mammals (Mesocricetus auratus) were studied by means methods lectin histochemistry. Goblet cells intestine showed different binding patterns depending upon location in villi. Thus, goblet located glands presented a reactivity from that at tip Differences stainings between small large intestines more marked higher...
The mammalian zona pellucida is an extracellular matrix surrounding the oocyte, and composed of three major glycoproteins, ZP1, ZP2, ZP3. Previous studies have suggested that sperm receptor activity resides in specific oligosaccharide chains on ZP3 glycoprotein. However, nature terminal monosaccharide(s) these glycosidic to which bind a matter active debate. Evidence has been presented support role for at least distinct monosaccharides binding, alpha-galactose, L-fucose Lewis X structures,...