A5000622730- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Veterinary Medicine and Surgery
- Reproductive System and Pregnancy
- Kruppel-like factors research
- Genetic and phenotypic traits in livestock
- RNA regulation and disease
- Evolution and Paleontology Studies
- Tissue Engineering and Regenerative Medicine
- COVID-19 diagnosis using AI
- Plant Reproductive Biology
- Menstrual Health and Disorders
- Bat Biology and Ecology Studies
- Animal Genetics and Reproduction
- Wildlife Ecology and Conservation
- COVID-19 and healthcare impacts
- Phagocytosis and Immune Regulation
- Urological Disorders and Treatments
- Insect and Pesticide Research
- Genetic Mapping and Diversity in Plants and Animals
- Pelvic floor disorders treatments
- Cell Adhesion Molecules Research
Instituto Murciano de Investigación Biosanitaria
2021-2024
Universidad de Murcia
2012-2024
Okayama University of Science
2014-2020
Physiologie de la Reproduction et des Comportements
2016-2019
Centre National de la Recherche Scientifique
2016-2018
Université de Tours
2016-2018
Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim this study was to evaluate the effect oviductal fluid possible involvement deleted in malignant brain tumor 1 (DMBT1) on IVF porcine equine species that represent divergent models. We first performed after pre-incubation oocytes with or without supplemented not antibodies directed against DMBT1. showed induces an increase monospermic rate is canceled by...
Mammalian eggs are surrounded by an extracellular matrix called the zona pellucida (ZP). This envelope participates in processes such as acrosome reaction induction, sperm binding, protection of oviductal embryo, and may be involved speciation. In eutherian mammals, this coat is formed three or four glycoproteins (ZP1–ZP4). While Mus musculus has been used a model to study ZP for more than 35 years, surprisingly, it only species which Zp1, Zp2, Zp3, Zp4 being pseudogene. was lost lineage...
Abstract Oviductal proteins play an important role in mammalian fertilization, as from seminal fluid. However, contrast with the latter, their phylogenetic evolution has been poorly studied. Our objective was to study 16 mammals of genes that encode oviductal involved at least one following steps: (1) sperm–oviduct interaction, (2) acrosome reaction, and/or (3) sperm–zona pellucida interaction. Most were present all studied mammals. some lost along and found pseudogenes: annexin A5 (ANXA5)...
The zona pellucida (ZP) is an extracellular envelope that surrounds mammalian oocytes. This coat participates in the interaction between gametes, induction of acrosome reaction, block polyspermy and protection oviductal embryo. Previous studies suggested carnivore ZP was formed by three glycoproteins (ZP2, ZP3 ZP4), with ZP1 being a pseudogene. However, recent study cat found all four proteins were expressed. In present study, silico molecular analyses performed several carnivores to clarify...
The zona pellucida (ZP) is an extracellular matrix that surrounds mammalian oocytes. In eutherians it formed from three or four proteins (ZP1, ZP2, ZP3, ZP4). the few marsupials have been studied, however, only of these characterised (ZP2, Nevertheless, composition in may be more complex, since a duplication ZP3 gene was recently described one species. aim this work to elucidate ZP and relate evolution family. For that, silico molecular analysis undertaken, focusing on two South American...
Abstract Recent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation expression and secretion porcine oviduct uterus, assesses its effect on vitro embryo production. A systematic was made sows different oestrous stages: late follicular, early luteal stages. Relative mRNA abundance quantified by RT-qPCR. In addition, analysed immunohistochemistry results were complemented...
Abstract In spite of many attempts to establish an in vitro fertilization (IVF) technique the equine, no efficient conventional IVF is available. The presence oviductal fluid or cells during helps improve embryo production but not sufficient reach high rates. Thus, our aim was perform equine either after sperm pre‐incubation with cells, and evaluate effect cumulus removal from oocyte progesterone. experiments 1 2, performed porcine oviduct epithelial cells. oocytes maturation tended increase...
Abstract Study question Is there a need for LH surge inhibition by GnRH-antagonists in luteal phase stimulation poor ovarian responders undergoing Duostim? Summary answer Inhibition of the progesterone during duostim protocols POR appears to be effective, safe and makes unnecessary. What is known already High levels have long been shown reversibly block associated with increased estradiol levels, reducing GnRH’s pulsatility from hypothalamus. This basis progestin-only contraceptives has...
OGP, encoded by the Ovgp1 gene, is major non-serum oviductal protein in most mammals. In genome of Rattus norvegicus, has been identified as a pseudogene. However, Mus musculus presents functional gene. As rat and mouse belong to subfamily Murinae, probably lost after their divergence. This study aims determine when pseudogenization event occurred which proteins could replace its function. To attain that, potential expression members belonging GH18 family investigated oviduct means molecular...
In a previous study, our group detected the cholecystokinin (CCK) protein in porcine oviduct. This fact, together with involvement of CCK regulation sperm tyrosine phosphorylation by modulation HCO3- uptake (in mice and humans) suggests role for during capacitation. Therefore, on one hand, expression receptors (CCK1R CCK2R) boar testes has been investigated probed; other spermatozoa (from seminal doses 1-day 5-day storage) were exposed to different concentrations (0-control, 25 or 50 μM)...
Colostrum performs nutritional, anti-inflammatory and anti-infective functions promotes immune system formation organ development. The new coronavirus, SARS-CoV-2, has generated concerns about viral transmission through human milk, with a lack of evidence milk’s protective effects against the infection. This study aimed at analyzing presence virus identifying protein expression profile colostrum in active COVID-19-recovered patients. samples were collected from women COVID-19 (n = 3),...
Abstract Crosstalk between the oocyte and surrounding cumulus cells (CCs) is essential for production of competent oocytes. Previous studies have analysed relative transcript abundance in oocytes derived from small (SF: <3 mm diameter)‐ medium‐sized (MF: 3–6 diameter) follicles to determine potential use SF‐derived assisted reproductive technologies (ART). The aim this study was examine CCs obtained cumulus–oocyte complexes (COCs) SF MF. Nine genes were selected according their importance...
In porcine cumulus-oocyte complexes (COC) from middle follicles (MF: 3–6 mm in diameter), FSH is known to induce the resumption of meiosis and accompanied by transactivate EGF receptor activation MAPK3/1 cumulus cells. The aim current study was examine effect oestradiol-17β (E2: 0.1 μg mL–1) or on vitro maturation (IVM) oocytes derived small (SF: 1–2 diameter). COC were aspirated MF ovaries obtained at slaughterhouse cultured for IVM mPOM (with 1 mM dibutyryl cAMP, 10 IU mL–1 eCG, hCG 20 h...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)