A5080701649- Parasite Biology and Host Interactions
- Helminth infection and control
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Aquaculture disease management and microbiota
- Parasites and Host Interactions
- Virus-based gene therapy research
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Genetics, Aging, and Longevity in Model Organisms
- Virology and Viral Diseases
- interferon and immune responses
- RNA Interference and Gene Delivery
- Nematode management and characterization studies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Invertebrate Immune Response Mechanisms
- Insect and Pesticide Research
- bioluminescence and chemiluminescence research
- Insects and Parasite Interactions
- Sesquiterpenes and Asteraceae Studies
- Mycobacterium research and diagnosis
- Parasitic Diseases Research and Treatment
- Cancer-related molecular mechanisms research
- Plant and Biological Electrophysiology Studies
Infectiologie Animale et Santé Publique
2012-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2022
Université de Tours
2014-2021
Institut National de la Recherche Agronomique
2011-2014
Virologie et Immunologie Moléculaires
2004-2013
DKFZ-ZMBH Alliance
2001
German Cancer Research Center
2001
University of Rochester
1998
University of Washington
1998
Inserm
1995-1998
Although Novirhabdovirus viruses, like the Infectious hematopietic necrosis virus (IHNV), have been extensively studied, limited knowledge exists on route of IHNV entry during natural infection. A recombinant (rIHNV) expressing Renilla luciferase gene was generated and used to infect trout. noninvasive bioluminescence assay developed so that replication in live fish could be followed hours after We provide here evidence fin bases are portal into fish. Confirmation brought by use a...
ABSTRACT ISG15, a 15-kDa interferon-induced protein that participates in antiviral defenses of mammals, is highly conserved among vertebrates. In fish, as viral infection and interferon treatment induce isg15 expression. The two ubiquitin-like domains ISG15 the presence consensus LRLRGG sequence C-terminal region, which required for covalent conjugation to substrate protein, are also fish. Our data demonstrate overexpression zebrafish (zf-ISG15) EPC cells sufficient inhibit by RNA viruses...
Cholinergic agonists such as levamisole and pyrantel are widely used anthelmintics to treat parasitic nematode infestations. These drugs elicit spastic paralysis by activating acetylcholine receptors (AChRs) expressed in body wall muscles. In the model Caenorhabditis elegans, genetic screens led identification of five genes encoding levamisole-sensitive-AChR (L-AChR) subunits: unc-38, unc-63, unc-29, lev-1 lev-8. subunits form a functional L-AChR when heterologously Xenopus laevis oocytes....
Previous studies have demonstrated the absence of viral replication Vif- mutants in stimulated primary blood mononuclear cells (PBMC). Human immunodeficiency virus type 1 strain NDK were propagated on semipermissive CEM cell line, and stock obtained was compared with wild-type during a single cycle PBMC. The able to enter PBMC same efficiency as wild type, by quantification strong-stop cDNA, retrotranscription observed for both viruses within 4 h postinfection. Using PCR assay an Alu-long...
Staphylococcus aureus, a major pathogen for the mammary gland of dairy ruminants, elicits recruitment neutrophils into milk during mastitis, but mechanisms are incompletely understood. We investigated response bovine to muramyl dipeptide (MDP), an elementary constituent bacterial peptidoglycan, alone or in combination with lipoteichoic acid (LTA), another staphylococcal microbial-associated molecular pattern (MAMP). MDP induced prompt and marked influx milk, its LTA elicited more intense...
The nonvirion (NV) protein of infectious hematopoietic necrosis virus (IHNV) has been previously reported to be essential for efficient growth and pathogenicity IHNV. However, little is known about the mechanism by which NV supports viral growth. In this study, cellular localization its role in IHNV host cells was investigated. Through transient transfection RTG-2 fused green fluorescent (GFP), a nuclear demonstrated. Deletion analyses showed that 32EGDL35 residues were protein, fusion these...
Acetylcholine receptors are pentameric ligand–gated channels involved in excitatory neuro-transmission both vertebrates and invertebrates. In nematodes, they represent major targets for cholinergic agonist or antagonist anthelmintic drugs. Despite the large diversity of acetylcholine-receptor subunit genes present only a few receptor subtypes have been characterized so far. Interestingly, parasitic nematodes affecting human animal health possess two closely related members this gene family,...
Detection of human immunodeficiency virus-type 1 (HIV-1) on only one or a few occasions in infants born to infected mothers has been interpreted indicate that infection may be transient rather than persistent. Forty-two cases suspected HIV-1 viremia among 1562 perinatally exposed seroreverting and mother were reanalyzed. env sequences not found specimens from 20; 6, somatic genetic analysis revealed mistakenly attributed an infant; 17, phylogenetic failed demonstrate the expected linkage...
Viral hemorrhagic septicaemia virus (VHSV) is an important viral pathogen in European rainbow trout farming. Isolates from wild marine fish and freshwater farms show highly different virulence profiles: isolates species cause little or no mortality following experimental waterborne challenge, whilst challenge with results high levels of mortality. Phylogenetic analyses have revealed that the virulent trout-derived evolved VHSV host over past 60 years. Recent reared zones intermediate...
Caprine arthritis encephalitis virus (CAEV) is a lentivirus closely related to visna and more distantly other lentiviruses, such as human immunodeficiency virus. The genomes of CAEV contain tat gene encoding protein able weakly transactivate its own long terminal repeat, suggesting that transactivation may be dispensable function for viral replication. Three different mutants an infectious molecular clone were used study their replication after transfection or infection primary goat synovial...
Macrocyclic lactones (MLs) are widely used drugs to treat and prevent parasitic nematode infections. In many species including a major pathogen of foals, Parascaris univalens, resistance against MLs is widespread, but the underlying mechanisms ML penetration routes into nematodes remain unknown. Here, we examined how P-glycoprotein efflux pumps, candidate genes for resistance, can modulate drug susceptibility investigated role active ingestion in model Caenorhabditis elegans. Wildtype or...
Complex retrovirus genomes contain a variable number of accessory genes, among which is the vif gene. We investigated in vitro role gene caprine arthritis encephalitis virus (CAEV) by studying phenotype five mutants after infection primary goat synovial membrane (GSM) cells and blood-derived monocytes/macrophages. Any deletion introduced into resulted slow low viral replication production virions with an infectious titer lower than that wild-type particles. The could be restored trans...
ABSTRACT We previously reported that infection of goats with caprine arthritis encephalitis virus (CAEV) tat − proviral DNA or results in persistent infection, since the animals seroconverted and direct isolation from cultures blood-derived macrophages was positive. In this study we wanted to determine whether injected CAEV were protected against challenge pathogenic homologous investigate still pathogenic. All became infected as indicated by seroconversion isolation. Challenge at 8 9 months...
A variant human immunodeficiency virus type 1 (HIV-1) vif gene, vifA45-2 , which encodes a protein lacking 19 amino acids at the C terminus but is fully functional in supporting HIV replication non-permissive cells has been described previously. By employing newly generated anti-VifA45 serum, further properties of VifA45 and its full-length counterpart, VifA45open, comparison to Vif from strain BH10 are reported permissive HeLa COS-7 cells. The results obtained using confocal microscopic...
Previously, we demonstrated that a representative M genogroup type strain of infectious haematopoietic necrosis virus (IHNV) from rainbow trout grows well in trout-derived RTG-2 cells, but U sockeye salmon has restricted growth, associated with reduced genome replication and mRNA transcription. Here, analysed further the mechanisms for this growth restriction U-type IHNV using strategies assessed differences viral genes, host immune regulation phosphorylation. To determine whether...
Marek's disease virus (MDV) is a growing threat for the poultry industry. Unfortunately, despite successful vaccination against disease, MDV remains in circulation within vaccinated flocks, leading to selection of increasingly virulent pathotypes. Detailed knowledge biology and host-virus interaction required improve vaccine efficiency. In present study, I engineered an original, dual-reporter track quantify replication vitro vivo.