A5051169392- Helminth infection and control
- Parasite Biology and Host Interactions
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Plant and fungal interactions
- Parasites and Host Interactions
- Nematode management and characterization studies
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Plant Parasitism and Resistance
- Chemical synthesis and alkaloids
- Nicotinic Acetylcholine Receptors Study
- Medicinal Plants and Neuroprotection
- Insect Resistance and Genetics
- Genetic and phenotypic traits in livestock
- Ruminant Nutrition and Digestive Physiology
- Insects and Parasite Interactions
- thermodynamics and calorimetric analyses
- Parasitic Diseases Research and Treatment
- GABA and Rice Research
- Insect Utilization and Effects
- Botanical Research and Chemistry
- Parasitic Infections and Diagnostics
- Gut microbiota and health
- Environmental DNA in Biodiversity Studies
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université de Tours
2015-2024
Infectiologie Animale et Santé Publique
2014-2023
Département Santé Animale
2023
Innovations thérapeutiques et résistances
2018-2019
CNR de la Résistance aux Antibiotiques
2018-2019
Cholinergic agonists such as levamisole and pyrantel are widely used anthelmintics to treat parasitic nematode infestations. These drugs elicit spastic paralysis by activating acetylcholine receptors (AChRs) expressed in body wall muscles. In the model Caenorhabditis elegans, genetic screens led identification of five genes encoding levamisole-sensitive-AChR (L-AChR) subunits: unc-38, unc-63, unc-29, lev-1 lev-8. subunits form a functional L-AChR when heterologously Xenopus laevis oocytes....
The nature and strength of interactions entertained among helminths their host gut microbiota remain largely unexplored. Using 40 naturally infected Welsh ponies, we tracked the microbiota-cyathostomin temporal dynamics stability before following anthelmintic treatment associated blood transcriptomic response. High shedders harbored 14 species cyathostomins, dominated by Cylicocyclus nassatus. They exhibited a highly diverse dynamic microbiota, with butyrate-producing Clostridia likely...
Nicotinic acetylcholine receptors (nAChRs) of parasitic nematodes are required for body movement and targets important "classical" anthelmintics like levamisole pyrantel, as well "novel" tribendimidine derquantel. Four biophysical subtypes nAChR have been observed electrophysiologically in muscle the nematode parasite Oesophagostomum dentatum, but their molecular basis was not understood. Additionally, loss one these (G 35 pS) found to be associated with resistance. In present study, we...
Resistance to the anthelmintic macrocyclic lactone ivermectin (IVM) has a great impact on control of parasitic nematodes. The mechanisms by which nematodes adapt IVM remain be deciphered. We have identified NHR-8, nuclear hormone receptor involved in xenobiotic response Caenorhabditis elegans, as new regulator tolerance IVM. Loss-of-function nhr-8(ok186) C. elegans mutants subjected larval development assays and electropharyngeogram measurements, displayed hypersensitivity IVM, silencing...
Basic knowledge on the biology and epidemiology of equine strongylid species still needs to be improved contribute design better parasite control strategies. Nemabiome metabarcoding is a convenient tool quantify identify in bulk samples that could overcome hurdle cyathostomin morphological identification represents. To date, this approach has relied internal transcribed spacer 2 (ITS-2) ribosomal RNA gene, with limited investigation its predictive performance for communities. Using DNA pools...
Acetylcholine receptors are pentameric ligand–gated channels involved in excitatory neuro-transmission both vertebrates and invertebrates. In nematodes, they represent major targets for cholinergic agonist or antagonist anthelmintic drugs. Despite the large diversity of acetylcholine-receptor subunit genes present only a few receptor subtypes have been characterized so far. Interestingly, parasitic nematodes affecting human animal health possess two closely related members this gene family,...
Summary Background and Purpose Control of nematode parasite infections relies largely on anthelmintic drugs, several which act nicotinic ACh receptors (nAChRs), there are concerns about the development resistance. There is an urgent need for new compounds to overcome resistance novel drug targets. We describe functional expression pharmacological characterization a homomeric nAChR, ACR‐16, from parasite. Experimental Approach Using RT‐PCR, molecular cloning two‐electrode voltage clamp...
The control of parasitic nematodes impacting animal health relies on the use broad spectrum anthelmintics. However, intensive these drugs has led to selection resistant parasites in livestock industry. In that respect, there is currently an urgent need for novel compounds able parasites. Nicotine also historically been used as a de-wormer but was removed from market when modern anthelmintics became available. pharmacological target nicotine identified acetylcholine-gated ion channels....
Abstract Alternative strategies to chemical anthelmintics are needed for the sustainable control of equine strongylids. Bioactive forages like sainfoin ( Onobrychis viciifolia ) could contribute reducing drug use, with first hints in vitro activity against cyathostomin free-living stages observed past. We analysed effect a sainfoin-rich diet on population and efficacy oral ivermectin treatment. Two groups 10 naturally infected horses were enrolled 78-day experimental trial. Following 1-week...
Abstract The growing range of drug resistant parasitic nematode populations threatens the sustainability ruminant farming worldwide. In this context, nutraceuticals, animal feed that provides necessary dietary requirements while ensuring parasite control, could contribute to increase in developed and low resource settings. study, we evaluated anthelmintic potential lupin seed extracts against major trichostrongylids, Haemonchus contortus Teladorsagia circumcincta . vitro observations showed...
The control of parasitic nematode infections relies mostly on anthelmintics. potential pharmacotherapeutic application phytochemicals, in order to overcome parasite resistance and enhance the effect existing drugs, is becoming increasingly important. antinematodal effects carveol was tested free-living
Ticks represent a major health issue for humans and domesticated animals. Exploring the expression landscape of tick's central nervous system (CNS), known as synganglion, would be an important step in understanding tick physiology managing tick-borne diseases, but studies on that topic are still relatively scarce. Neuron-specific genes like cys-loop ligand-gated ion channels (cys-loop LGICs, or cysLGICs) pharmacological targets acaricides. To date their sequence have not been well catalogued...
In the absence of efficient alternative strategies, control parasitic nematodes, impacting human and animal health, mainly relies on use broad-spectrum anthelmintic compounds. Unfortunately, most these drugs have a limited single-dose efficacy against infections caused by whipworm, Trichuris. These are both veterinary importance. However, in contrast to wide range nematode species, narrow-spectrum oxantel has high Trichuris spp. Despite this knowledge, molecular target(s) within is still...
Background and Purpose Macrocyclic lactones are the most widely used broad‐spectrum anthelmintic drugs for treatment of parasitic nematodes affecting both human animal health. agonists nematode glutamate‐gated chloride channels (GluCls). However, many important species, GluCls subunit composition pharmacological properties remain largely unknown. To gain new insights into GluCl diversity mode action macrocyclic lactones, we identified pharmacologically characterized receptors made highly...
Natural plant compounds, such as betaine, are described to have nematocidal properties. Betaine also acts a neurotransmitter in the free-living model nematode
The current control of gastrointestinal (GI) parasitic nematodes mainly relies on the widespread use anthelmintics, which has inevitably led to resistance. Therefore, there is an urgent need find new sources antiparasitic compounds. Macroalgae represent a rich source active molecules and are widely described as having medicinal properties. In present study, we investigated potential anthelmintic activity aqueous extracts from three species algae (Bifurcaria bifurcata, Grateloupia turuturu...
Cyathostomins are a complex of 50 intestinal parasite species infecting horses and wild equids. The massive administration modern anthelmintic drugs has increased their relative abundance in horse helminth communities selected drug-resistant isolates worldwide. Cylicocyclus nassatus is the most prevalent abundant species. tedious identification isolation these worms have hampered studies biology that remain largely uncharacterised. Here we leveraged ultra-low input sequencing protocols to...
<title>Abstract</title> The nature and strength of interactions entertained among helminths their host gut microbiota remain largely unexplored. Using 40 naturally infected Welsh ponies, we tracked the microbiota-cyathostomin temporal dynamics during parasite community removal reassembly, associated immune response. Infected ponies harboured 14 species cyathostomins, overwhelmed by abundance Cylicocyclus nassatus. Parasite carriers exhibited environment modifications, higher Shannon entropy...
Abstract Basic knowledge on the biology and epidemiology of equine strongylid species remains insufficient although it would contribute to design better parasite control strategies. Nemabiome is a convenient tool quantify identify in bulk samples that could overcome hurdle cyathostomin morphological identification represents. To date, this approach has relied internal transcribed spacer 2 (ITS-2) ribosomal RNA cistron its predictive performance associated biases both remain unaddressed. This...
Abstract Glutamate-gated chloride channels receptors (GluCls) are involved in the inhibition of neurotransmission invertebrates and represent major molecular targets for therapeutic drugs. Among these drugs, macrocyclic lactones (MLs) widely used as anthelmintic to treat parasitic nematodes impacting both human animal health. Despite massive use MLs since 80’s, exact drugs still unknown many important parasite species. GluCl subunit encoding genes, avr-14 , glc-2 glc-3 glc-4 highly conserved...
Abstract The growing expansion range of drug resistant parasitic nematode populations threatens the sustainability ruminant farming worldwide. In this context, nutraceuticals, i.e. feed that would both fulfil dietary requirements while ensuring parasite control, contribute to increase in developed and low resource settings. study, we characterized anthelmintic potential lupin seed extracts against major trichostrongylids, H. contortus T. circumcincta . Our observations showed total from...