A5066120393- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Soybean genetics and cultivation
- Parasite Biology and Host Interactions
- Entomopathogenic Microorganisms in Pest Control
- Forest Insect Ecology and Management
- Agronomic Practices and Intercropping Systems
- Plant Pathogens and Resistance
- Chromosomal and Genetic Variations
- Genetic and Environmental Crop Studies
- Plant and animal studies
- Plant Parasitism and Resistance
- Agriculture and Rural Development Research
- Helminth infection and control
- Genetic diversity and population structure
- Plant Disease Management Techniques
- Oil Palm Production and Sustainability
- Plant Pathogens and Fungal Diseases
- Insect-Plant Interactions and Control
- Banana Cultivation and Research
- Insect Pest Control Strategies
- Plant Disease Resistance and Genetics
- Studies on Chitinases and Chitosanases
- Phytase and its Applications
Institut Sophia Agrobiotech
2015-2025
Centre National de la Recherche Scientifique
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2025
Université Côte d'Azur
2013-2025
Observatoire de la Côte d’Azur
2017-2019
Département Santé Animale
2016
Institut National de la Recherche Agronomique
2000-2015
Laboratoire des Interactions Plantes Micro-Organismes
1999-2009
Museo Nacional de Ciencias Naturales
2001
Département Santé des Plantes et Environnement
2000
Plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them sorely needed. We report the draft genome sequence of root-knot nematode Meloidogyne incognita, a biotrophic parasite many crops, including tomato, cotton coffee. Most assembled this asexually reproducing nematode, totaling 86 Mb, exists in pairs homologous but divergent segments. This suggests that ancient allelic regions M. incognita evolving toward effective haploidy, permitting new...
Here, the interaction of Melodoigyne incognita virulent and avirulent pathotypes with susceptible Mi-resistant tomato (Solanum lycopersicon) has been studied. Significant differences in nematode penetration occurred 2 days postinoculation (dpi) became stable from 3 dpi onwards. The hypersensitive cell response (HR) resistant plants prevented installation pathotype. pathotype overcame Mi (nematode) resistance induced feeding sites root cells without triggering HR. Reactive oxygen species...
Abstract Microsatellites (or SSRs: simple sequence repeats) are among the most frequently used DNA markers in many areas of research. The use microsatellite is limited by difficulties involved their de novo isolation from species for which no genomic resources available. We describe here a high‐throughput method isolating based on coupling multiplex enrichment and next‐generation sequencing 454 GS‐FLX Titanium platforms. procedure was calibrated model ( Apis mellifera ) validated 13 other...
SUMMARY Taxonomy: Eukaryota; Metazoa; Nematoda; Chromadorea; order Tylenchida; Tylenchoidea; Heteroderidae; genus Meloidogyne. Physical properties: Microscopic‐non‐segmented worms. Meloidogyne species can reproduce by apomixis, facultative meiotic parthenogenesis or obligate mitotic parthenogenesis. Obligate biotrophic parasites inducing the re‐differentiation of plant cells into specialized feeding cells. Hosts: spp. infest more than 3000 including vegetables, fruit trees, cereals and...
RAPD markers were used to characterize the genetic diversity and relationships of root-knot nematodes (RKN) (Meloidogyne spp.) in Brazil. A high level infraspecific polymorphism was detected Meloidogyne arenaria, exigua, hapla compared with other species tested. Phylogenetic analyses showed that M. exigua are more closely related one another than they species, illustrated early divergence these meiotically reproducing from mitotic ones. To develop a PCR-based assay specifically identify RKN...
Root-knot nematodes (genus Meloidogyne) exhibit a diversity of reproductive modes ranging from obligatory sexual to fully asexual reproduction. Intriguingly, the most widespread and devastating species global agriculture are those that reproduce asexually, without meiosis. To disentangle this surprising parasitic success despite absence sex genetic exchanges, we have sequenced assembled genomes three ameiotic Meloidogyne. We compared them relatives able perform meiosis show Meloidogyne...
Meloidogyne enterolobii (= M. mayaguensis ), the root-knot nematode of pacara earpod tree, belongs to group tropical nematodes and is considered as one most damaging species, due its wide host range, pathogenicity ability develop reproduce on several crops carrying resistance genes. Moreover, recent reports indicate that geographic distribution parasite tends extend beyond areas, risk establishment spread in Mediterranean regions southern Europe now highly probable. Recently, molecular...
Similarly to microbial pathogens, plant-parasitic nematodes secrete into their host plants proteins that are essential establish a functional interaction. Identifying the destination of nematode secreted within plant cell compartment(s) will provide compelling clues on molecular functions. Here fine localization five was analysed throughout parasitism in Arabidopsis thaliana. An immunocytochemical method developed preserves both and pathogen tissues, allowing organisms. One protein from...
Root‐knot nematodes ( Meloidogyne spp.) are among the main pathogens of tomato Lycopersicon esculentum ) worldwide. Plant resistance is currently method choice for controlling these pests and all commercially available resistant cultivars carry dominant Mi gene, which confers to three species arenaria , M. incognita javanica . However emergence virulent biotypes able overcome gene may constitute a severe limitation such control strategy. To date, little was known possible influence...
The objectives of this work were to evaluate the genetic variability Meloidogyne enterolobii by molecular markers, and develop species‐specific markers for application in detection. Sixteen M. isolates from different geographical regions (Brazil other countries) hosts used study. identification purification populations carried out based on isoenzyme phenotype. DNA amplification intergenic region (IGS) rDNA between cytochrome oxidase subunit II ( COII ) 16S rRNA genes (mtDNA) produced...
Resistant cultivars are key elements for pathogen control and pesticide reduction, but their repeated use may lead to the emergence of virulent populations, able overcome resistance. Increased research efforts, mainly based on theoretical studies, explore spatio-temporal deployment strategies resistance genes in order maximize durability. We evaluated experimentally three these root-knot nematodes: cultivar mixtures, alternating pyramiding genes, under controlled field conditions over a...
Cholinergic agonists such as levamisole and pyrantel are widely used anthelmintics to treat parasitic nematode infestations. These drugs elicit spastic paralysis by activating acetylcholine receptors (AChRs) expressed in body wall muscles. In the model Caenorhabditis elegans, genetic screens led identification of five genes encoding levamisole-sensitive-AChR (L-AChR) subunits: unc-38, unc-63, unc-29, lev-1 lev-8. subunits form a functional L-AChR when heterologously Xenopus laevis oocytes....
Amplified fragment length polymorphism fingerprinting of three pairs Meloidogyne incognita near-isogenic lines (NILs) was used to identify markers differential between nematode genotypes avirulent or virulent against the tomato Mi resistance gene. One these sequences, present only in lines, as a probe screen cDNA library from second-stage juveniles (J2s) and allowed cloning encoding secretory protein. The putative full-length cDNA, named map-1, encoded 458 amino acid (aa) protein containing...
A new root-knot nematode parasitising vegetables, flowers and fruits in Brazil, Iran Chile, is described as Meloidogyne luci n. sp. The female has an oval to squarish perineal pattern with a low moderately high dorsal arc without shoulders, similar M. ethiopica . stylet robust 15-16 μm long; the distance from pharyngeal gland orifice base (DGO) 3-4 μm. Males have high, rounded head cap continuous body contour. labial disc fused medial lips form elongated lip structure. region not marked by...
Adaptation to changing environmental conditions represents a challenge parthenogenetic organisms, and until now, how phenotypic variants are generated in clones response the selection pressure of their environment remains poorly known. The obligatory root-knot nematode species Meloidogyne incognita has worldwide distribution is most devastating plant-parasitic nematode. Despite its asexual reproduction, this exhibits an unexpected capacity adaptation constraints, for example, resistant...
Abstract In an era marked by rapid global changes, the reinforcement and modernization of plant health surveillance systems have become imperative. Sixty-five scientists present here a research agenda for enhanced modernized to anticipate mitigate disease pest emergence. Our approach integrates wide range scientific fields (from life, social, physical engineering sciences) identifies key knowledge gaps, focusing on anticipation, risk assessment, early detection, multi-actor collaboration....