A5027957589- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and Fungal Interactions Research
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Virus-based gene therapy research
- Insect and Arachnid Ecology and Behavior
- Mosquito-borne diseases and control
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Forest Insect Ecology and Management
- Entomopathogenic Microorganisms in Pest Control
- Invertebrate Immune Response Mechanisms
- Insect-Plant Interactions and Control
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Plant Pathogens and Resistance
- Insect behavior and control techniques
- CRISPR and Genetic Engineering
- Entomological Studies and Ecology
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Species Distribution and Climate Change
- Rabies epidemiology and control
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Université de Montpellier
2012-2024
Diversité, génomes et interactions micro-organismes-insectes
2015-2024
University of Oxford
2022
Institut Agro Montpellier
2009-2015
Interdisciplinarité en Santé Publique Interventions et Instruments de mesure complexes – Région Est
2014
Microbiologie de l’alimentation au service de la santé
2014
Institut National de la Recherche Agronomique
2013
Laboratoire d’Ethologie Expérimentale et Comparée
2005
Centre National de la Recherche Scientifique
1993-2003
Members of the family Parvoviridae are small, resilient, non-enveloped viruses with linear, single-stranded DNA genomes 4-6 kb. Viruses in two subfamilies, Parvovirinae and Densovirinae, distinguished primarily by their respective ability to infect vertebrates (including humans) versus invertebrates. Being genetically limited, most parvoviruses require actively dividing host cells and/or tissue specific. Some cause diseases, which range from subclinical lethal. A few co-infection helper...
Abstract Our knowledge of the genetic diversity and host ranges viruses is fragmentary. This particularly true for Parvoviridae family. Genetic studies single stranded DNA within this family have been largely focused on arthropod- vertebrate-infecting species that cause diseases humans our domesticated animals: a focus has biased perception parvovirus diversity. While metagenomics approaches could help rectify bias, so too transcriptomics studies. Large amounts transcriptomic data are...
Over the last decade, viral metagenomic studies have resulted in discovery of thousands previously unknown viruses. These are likely to play a pivotal role obtaining an accurate and robust understanding how viruses affect stability productivity ecosystems. Among metagenomics-based approaches that been developed since beginning 21st century, shotgun metagenomics applied specifically virion-associated nucleic acids (VANA) has used disentangle diversity world. We summarize herein results 24...
Abstract In an era marked by rapid global changes, the reinforcement and modernization of plant health surveillance systems have become imperative. Sixty-five scientists present here a research agenda for enhanced modernized to anticipate mitigate disease pest emergence. Our approach integrates wide range scientific fields (from life, social, physical engineering sciences) identifies key knowledge gaps, focusing on anticipation, risk assessment, early detection, multi-actor collaboration....
Polyhedrin and p10 genes are expressed concurrently during the late stage of infection. To determine whether any competition occurs between these two at a transcriptional and/or translational level, series Autographa californica nuclear polyhedrosis recombinant viruses with deletions promoter coding sequences or polyhedrin gene was constructed. Two modified baculoviruses only one very promoters, single late-promoter AcSLP10 AcSLP33, were made. The used as reporter to allow direct comparison...
Spodoptera frugiperda (Noctuidae) is a major agricultural pest throughout the American continent. The highly polyphagous larvae are frequently devastating crops of importance such as corn, sorghum, cotton and grass. In addition, Sf9 cell line, widely used in biochemistry for vitro protein production, derived from S. tissues. Many research groups using model organism to investigate questions plant adaptation, behavior or resistance pesticides. this study, we constructed reference...
Densoviruses are parvoviruses that can be lethal for insects of different orders at larval stages. Although the horizontal transmission mechanisms poorly known, densoviral pathogenesis usually starts with ingestion contaminated food by host. Depending on virus, this leads to replication restricted midgut or excluding it. In both cases success infection depends virus capacity enter intestinal epithelium. Using Junonia coenia densovirus (JcDNV) as prototype and lepidopteran host Spodoptera...
Viral metagenomics and high throughput sequence mining have revealed unexpected diversity, the potential presence, of parvoviruses in animals from all phyla. Among arthropods, this diversity highlights poor knowledge that we regarding evolutionary history densoviruses. The aim study was to explore densovirus a small arthropod pest belonging Acari, two-spotted spider mite Tetranychus urticae, while using viral based on virus-enrichment. Here, present viromes obtained T. urticae laboratory...
ABSTRACT Junonia coenia densovirus (JcDNV) is an ambisense insect parvovirus highly pathogenic for lepidopteran pests at larval stages. The potential use of DNVs as biological control agents prompted us to reinvestigate the host range and cellular mechanisms infection. In order understand early events infection, we set up a functional infection assay in cell line pest Lymantria dispar determine intracellular pathway undertaken by JcDNV infect permissive line. Our results show that particles...
A whole set of Expressed Sequence Tags (ESTs) from the Sf9 cell line Spodoptera frugiperda is presented here for first time. By this way we want to identify both conserved and specific genes pest species. We also expect analysis find a class protein sequences providing tool explore genomic features phylogeny Lepidoptera.The ESTs display housekeeping as well developmentally regulated genes, high percentage with unknown function. Among identified ORFs, almost all ribosomal proteins (RPs) were...
Abstract Urban Aedes mosquitoes are vectors of many viruses affecting human health such as dengue, chikungunya and Zika viruses. Insecticide resistance environmental toxicity risks hamper the effectiveness chemical control against these mosquito vectors. Alternative methods, use mosquito-specific entomopathogenic should be explored. Numerous studies have focused on evaluating potential different densoviruses species biological agents. However, knowledge extent inter- intra-specific...
ABSTRACT Densoviruses are insect parvoviruses that orally infectious for Lepidoptera . To assess the mechanisms underlying their specificity and virulence, we investigated role of eight candidate residues in densovirus capsid. We showed substitutions four amino acids were associated with decreased virulence due to a ability cross host midgut epithelium, without an effect on viral replication other tissues.
Advances in viral metagenomics have paved the way of virus discovery by making exploration viruses any ecosystem possible. Applied to agroecosystems, such an approach opens new possibilities explore how circulate between insects and plants, which may help optimise their management. It could also lead identifying novel entomopathogenic resources potentially suitable for biocontrol strategies. We sampled larvae a natural population alfalfa weevils (Hypera postica), major herbivorous pest...
Viral infections of alfalfa are widespread in major cultivation areas and their impact on production may be underestimated. A new viral species, provisionally named virus F (AVF), was identified using a virion-associated nucleic acid (VANA) metagenomics-based approach (Medicago sativa L.) samples collected Southern France. The nucleotide sequence the genome determined by de-novo assembly VANA reads 5'/3' RACE with RNA extracted from enriched particles or total RNA, respectively. shares...
ABSTRACT Densoviruses (DVs) infect arthropods and belong to the Parvoviridae family. Here, we report complete coding sequence of a novel DV isolated from plant Hordeum marinum ( Poaceae ) by viral metagenomics, confirmed reamplification PCR. Phylogenetic analyses showed that this is related genus Iteradensovirus .
Insect innate immunity relies on numerous soluble and membrane-bound receptors, named pattern recognition proteins (PRPs), which enable the insect to recognize pathogen-associated molecular patterns. C-type lectins are among best-studied PRPs constitute most diverse family of animal lectins. Here we have characterized two groups Spodoptera exigua that differ in their phylogeny, domain architecture, expression pattern. One group includes with similar characteristics other lepidopteran...
The phosphatidylinositol-3-kinase (PI3K)/Akt/target of rapamycin (TOR) signalling pathway controls cell growth and survival, is targeted by a number viruses at different phases their infection cycle to control translation. Whether how insect interact with this remain poorly addressed. Here, we investigated the role PI3K/Akt/TOR during lethal cells an parvovirus. Using Junonia coenia densovirus (JcDV; lepidopteran ambidensovirus 1) susceptible as experimental models, first described JcDV...