A5033485574- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Nematode management and characterization studies
- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
- Agronomic Practices and Intercropping Systems
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Insect-Plant Interactions and Control
- Viral gastroenteritis research and epidemiology
- Insect symbiosis and bacterial influences
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Evolution and Genetic Dynamics
- Cocoa and Sweet Potato Agronomy
- Bioenergy crop production and management
- Photosynthetic Processes and Mechanisms
- Antibiotic Use and Resistance
- Genetics, Aging, and Longevity in Model Organisms
- Advanced biosensing and bioanalysis techniques
- Plant and soil sciences
Centre for Plant Biotechnology and Genomics
2025
Universidad Politécnica de Madrid
2025
University of Liège
2016-2024
Gembloux Agro-Bio Tech
2018-2024
Michigan State University
2022
Terra
2021
Centre Hospitalier Universitaire de Liège
2021
AgroBio
2016-2019
Institut Jean-Pierre Bourgin
2017
Université Paris-Saclay
2017
Recent developments in high-throughput sequencing (HTS), also called next-generation (NGS), technologies and bioinformatics have drastically changed research on viral pathogens spurred growing interest the field of virus diagnostics. However, reliability HTS-based detection protocols must be evaluated before adopting them for Many different algorithms aimed at detecting viruses HTS data been reported but little attention has paid thus far to their sensitivity diagnostic purposes. Therefore,...
Summary The ability to address the CRISPR ‐Cas9 nuclease complex any target DNA using customizable single‐guide RNA s has now permitted genome engineering in many species. Here, we report its first successful use a nonvascular plant, moss Physcomitrella patens . Single‐guide (sg s) were designed an endogenous reporter gene, Pp APT , whose inactivation confers resistance 2‐fluoroadenine. Transformation of protoplasts with these sg and Cas9 coding sequence from Streptococcus pyogenes triggered...
Albugo spp. are biotrophic parasites that cause white rust in Brassicaceae species, with significant crop losses. The generalist A. candida and the specialist laibachii infect Arabidopsis thaliana, pathosystem Albugo-Arabidopsis is a model for research molecular genetics of plant-pathogen interactions. occurrence infection by wild populations data on resistance-susceptibility compatible hypothesis host-pathogen coevolution. However, negative impact Arabidopsis-a requirement coevolution-has...
Abstract High‐throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in sample. The growing interest application HTS technologies for routine diagnostics plant health laboratories triggering development guidelines on how to prepare performing testing. This paper describes general technical recommendations guide through complex process preparing laboratory tests within existing quality assurance systems....
High-throughput sequencing (HTS) technologies have the potential to become one of most significant advances in molecular diagnostics. Their use by researchers detect and characterize plant pathogens pests has been growing steadily for more than a decade they are now envisioned as routine diagnostic test be deployed pest diagnostics laboratories. Nevertheless, HTS downstream bioinformatics analysis generated datasets represent complex process including many steps whose reliability must...
Over the last decade, viral metagenomic studies have resulted in discovery of thousands previously unknown viruses. These are likely to play a pivotal role obtaining an accurate and robust understanding how viruses affect stability productivity ecosystems. Among metagenomics-based approaches that been developed since beginning 21st century, shotgun metagenomics applied specifically virion-associated nucleic acids (VANA) has used disentangle diversity world. We summarize herein results 24...
Modern agriculture has influenced plant virus emergence through ecosystem simplification, introduction of new host species, and reduction in crop genetic diversity. Therefore, it is crucial to better understand distributions across cultivated uncultivated communities agro-ecological interfaces, as well exchange among them. Here, we advance fundamental understanding this area by characterizing the virome three co-occurring replicated Poaceae community types that represent a gradient grass...
(1) Background: In the current context of COVID-19 crisis, there is a need for fast, easy-to-use, and sensitive diagnostic tools in addition to molecular methods. We have therefore decided evaluate performance newly available antigen detection kits “real-life” laboratory conditions. (2) Methods: The sensitivity specificity two rapid tests (RDT)—the Ag Respi-Strip from Coris Bioconcept, Belgium (CoRDT), coronavirus test cassette Healgen Scientific, LLC, USA (HeRDT)—were evaluated on 193...
High throughput sequencing of total RNA isolated from symptomatic leaves a sweet cherry tree (Prunus avium cv. 0900 Ziraat) Turkey identified new member the genus Robigovirus designated virus (CVTR). The presence was confirmed by electron microscopy and overlapping RT-PCR for its whole-genome. has ssRNA genome 8464 nucleotides which encodes five open reading frames (ORFs) comprises two non-coding regions, 5′ UTR 3′ 97 296 nt, respectively. Compared to most closely related robigoviruses,...
Although chestnut mosaic disease (ChMD) was described several decades ago, its etiology is still not clear. Using classical approaches and high-throughput sequencing (HTS) techniques, we identified a novel Badnavirus that strong etiological candidate for ChMD. Two sources from Italy France were submitted to HTS-based viral indexing. Total RNAs extracted, ribodepleted, sequenced on an Illumina NextSeq500 (2 × 150 nt or 2 75 nt). In each source, single contig of ≈7.2 kb corresponds complete...
Cassava is one of the most important staple crops in Africa and its production seriously damaged by viral diseases. In this study, we identify for first time characterize genome organization novel ampeloviruses infecting cassava plants diverse geographical locations using three high-throughput sequencing protocols [Virion-Associated Nucleotide Acid (VANA), dsRNA total RNA], provide a analysis diversity these agents evolutionary forces acting on them. Thirteen new Closteroviridae isolates...
Wild plants serve as a large reservoir of known and yet-unknown viruses source viral pathogens cultivated plants. Yellow mosaic disease forest shrub Ligustrum vulgare (privet) was recurrently observed in Europe for more than 100 years. Using universal virus identification approach based on deep sequencing de novo assembly small interfering (si)RNAs we identified causative agent this Switzerland reconstructed its complete 3-segmented RNA genome. Notably, short 3'-terminal common region (CR)...
Giant goldenrod (Solidago gigantea, Asteraceae) is a rhizomatous herbaceous perennial native to North America. Introduced Europe in the 18th century as an ornamental, S. gigantea now considered highly invasive, exerting negative impacts on plant communities (Weber & Jakobs, 2005; Pal et al., 2015). In summer 2023, survey was done Belgium better characterise viruses infecting giant goldenrod. Fifty asymptomatic plants were collected from ten sites at roadside and garden edges centre of (the...
Pawpaw (Asimina triloba (L.) Dunal, Annonaceae) is a fruit tree native to eastern North America, increasingly grown for commercial production in the United States (Callaway, 1992; Layne, 1996), Europe, and Western Asia (Brannan Coyle, 2021; Lolletti et al., 2021). In 2012, virus-like symptoms were noticed 0.3 ha pawpaw orchard at Michigan State University Plant Pathology Research Station; ~30% of trees presented which included foliar mosaic, vein yellowing, necrosis, first mistaken nutrient...