A5046672506- Genetically Modified Organisms Research
- Insect Resistance and Genetics
- Agricultural safety and regulations
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Animal Genetics and Reproduction
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Intellectual Property and Patents
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- DNA Repair Mechanisms
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Light effects on plants
- Insect symbiosis and bacterial influences
- Bryophyte Studies and Records
- Plant Stress Responses and Tolerance
- Plant and animal studies
- Plant Gene Expression Analysis
- Lichen and fungal ecology
- Legume Nitrogen Fixing Symbiosis
- Food Allergy and Anaphylaxis Research
Institut Jean-Pierre Bourgin
2016-2025
AgroParisTech
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2025
Université Paris-Saclay
2016-2025
University of Zurich
2017-2023
Centre National de la Recherche Scientifique
2008-2020
Institut National de la Recherche Agronomique
1996-2019
Groupe d’Étude de la Matière Condensée
2017
Centre Île-de-France - Versailles-Grignon
2016
Département Biologie et Amélioration des Plantes
2003-2011
Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop improvement. The current technical challenge is to efficiently induce precise predictable targeted point mutations valuable breeding purposes. Cytidine base editors (CBEs) are CRISPR/Cas9 derived recently developed direct a C-to-T conversion. Stable genomic integration of components through Agrobacterium-mediated transformation the most widely used approach in dicotyledonous plants....
In many plant species, gene dosage is an important cause of phenotype variation. Engineering dosage, particularly in polyploid genomes, would provide efficient tool for breeding. The hexaploid oilseed crop Camelina sativa, which has three closely related expressed subgenomes, ideal species investigation the possibility creating a large collection combinatorial mutants. Selective, targeted mutagenesis delta-12-desaturase (FAD2) genes was achieved by CRISPR-Cas9 editing, leading to reduced...
Strigolactones are a novel class of plant hormones controlling shoot branching in seed plants. They also signal host root proximity during symbiotic and parasitic interactions. To gain better understanding the origin strigolactone functions, we characterised moss mutant strongly affected biosynthesis following deletion CAROTENOID CLEAVAGE DIOXYGENASE 8 (CCD8) gene. Here, show that wild-type Physcomitrella patens produces releases strigolactones into medium where they control protonemal...
This document provides supplementary guidance on specific topics for the allergenicity risk assessment of genetically modified plants. In particular, it supplements general recommendations outlined in previous EFSA GMO Panel guidelines and Implementing Regulation (EU) No 503/2013. The addressed are non-IgE-mediated adverse immune reactions to foods, vitro protein digestibility tests endogenous allergenicity. New scientific regulatory developments regarding these three described this...
Summary In many crop species, natural variation in eIF 4E proteins confers resistance to potyviruses. Gene editing offers new opportunities transfer genetic crops that seem lack alleles. However, because are physiologically important proteins, any introduced modification for virus must not bring adverse phenotype effects. this study, we assessed the role of amino acid substitutions encoded by a Pisum sativum virus‐resistance allele (W69L, T80D S81D, S84A, G114R and N176K) introducing them...
Phosphatidylinositol (PtdIns) transfer proteins (PITPs) regulate signaling interfaces between lipid metabolism and membrane trafficking. Herein, we demonstrate that AtSfh1p, a member of large uncharacterized Arabidopsis thaliana Sec14p-nodulin domain family, is PITP regulates specific stage in root hair development. AtSfh1p localizes along the plasma enriched discrete domains tip cytoplasm. This localization pattern recapitulates visualized for PtdIns(4,5)P2 developing hairs. Gene ablation...
Meiotic recombination is initiated by the formation of numerous DNA double-strand breaks (DSBs) catalysed widely conserved Spo11 protein. In Saccharomyces cerevisiae, requires nine other proteins for meiotic DSB formation; however, unlike Spo11, few these are across kingdoms. order to investigate this step in higher eukaryotes, we took advantage a high-throughput mutant screen carried out model plant Arabidopsis thaliana. A collection 55,000 lines was screened, and spo11-like mutations,...
Cloning through seeds has potential revolutionary applications in agriculture, because it would allow vigorous hybrids to be propagated indefinitely. However, asexual seed formation or apomixis, avoiding meiosis and fertilization, is not found the major food crops. To develop de novo synthesis of we crossed Arabidopsis MiMe dyad mutants that produce diploid clonal gametes a strain whose chromosomes are engineered eliminated after fertilization. Up 34% progeny were clones their parent,...
Abstract Powerful genome editing technologies are needed for efficient gene function analysis. The CRISPR-Cas9 system has been adapted as an gene-knock-out technology in a variety of species. However, number situations, knocking out or modifying single is not sufficient; this particularly true genes belonging to common family, showing redundant functions. Like many plants, the model organism Physcomitrella patens experienced multiple events polyploidization during evolution that resulted...
Summary The ability to address the CRISPR ‐Cas9 nuclease complex any target DNA using customizable single‐guide RNA s has now permitted genome engineering in many species. Here, we report its first successful use a nonvascular plant, moss Physcomitrella patens . Single‐guide (sg s) were designed an endogenous reporter gene, Pp APT , whose inactivation confers resistance 2‐fluoroadenine. Transformation of protoplasts with these sg and Cas9 coding sequence from Streptococcus pyogenes triggered...
High-throughput RNA sequencing (RNA-seq) has recently become the method of choice to define and analyze transcriptomes. For model moss Physcomitrella patens, although this been used help specific perturbations, no overall reference dataset yet established. In framework Gene Atlas project, Joint Genome Institute selected P. patens as a flagship genome, opening way generate first comprehensive transcriptome for moss. The round described here is composed 99 independent libraries spanning 34...
This Scientific Opinion addresses the formulation of specific development needs, including research requirements for allergenicity assessment and protein safety, in general, which is urgently needed a world that demands more sustainable food systems. Current risk strategies are based on principles guidelines Codex Alimentarius safety foods derived from 'modern' biotechnology initially published 2003. The core approach 'weight-of-evidence' because no single piece information or experimental...
In eukaryotes the photosynthetic antenna system is composed of subunits encoded by light harvesting complex (Lhc) multigene family. These proteins play a key role in photosynthesis and are involved both photoprotection. The moss Physcomitrella patens member lineage that diverged from seed plants early after land colonization therefore studying this organism, we may gain insight into adaptations to aerial environment.In study, characterized protein family patens, sequence analysis as well...
Nucleocytoplasmic large DNA viruses (NCLDVs) are eukaryotic with genomes (100 kb-2.5 Mb), which include giant Mimivirus, Megavirus and Pandoravirus. NCLDVs known to infect animals, protists phytoplankton but were never described as pathogens of land plants. Here, we show that the bryophyte Physcomitrella patens lycophyte Selaginella moellendorffii have open reading frames (ORFs) high phylogenetic affinities NCLDV homologues. The P. genes clustered in stretches (up 13 kb) containing up 16...
Since its discovery and first applications for genome editing in plants, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 technology has revolutionized plant research precision crop breeding. Although classical CRISPR-Cas9 system is a highly efficient tool disruptive targeted mutagenesis, this mostly inefficient introduction of precise predictable nucleotide substitutions. Recently, Prime Editing been developed, allowing simultaneous generation transitions...
Genome editing has become a major tool for both functional studies and plant breeding in several species. Besides generating knockouts through the classical CRISPR-Cas9 system, recent development of CRISPR base holds great exciting opportunities production gain-of-function mutants. The PAM requirement is strong limitation technologies such as editing, because substitution mainly occurs small edition window. As precise single amino-acid can be responsible functions associated to some domains...
This statement supplements and updates the GMO Panel guidance document on allergenicity of genetically modified (GM) plants published in 2017. In that document, considered additional investigations
Abstract In 2012, EFSA issued an opinion on plants developed through cisgenesis and intragenesis. With the development of New Genomic Techniques (NGTs) in last decade, cisgenic intragenic can now be obtained with insertion a desired sequence precise location genome. has been requested by European Commission to provide updated scientific safety risk assessment intragenesis, order (i) identify potential risks, comparing them those posed conventional breeding Established (EGTs) (ii) determine...