A5004472870- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- MicroRNA in disease regulation
- Insect-Plant Interactions and Control
- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Advanced biosensing and bioanalysis techniques
- Plant Genetic and Mutation Studies
- Plant Parasitism and Resistance
- Plant Disease Management Techniques
- Plant nutrient uptake and metabolism
- Phytoplasmas and Hemiptera pathogens
- Insect Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Enzyme Production and Characterization
- Animal Virus Infections Studies
- RNA Research and Splicing
- Plant Pathogens and Resistance
- Genetics, Bioinformatics, and Biomedical Research
Plant Protection Institute
2021-2025
Magyar Agrár- és Élettudományi Egyetem
2021-2025
Agricultural Biotechnology Institute
2010-2020
National Agricultural Research and Innovation Centre
2014-2020
Gembloux Agro-Bio Tech
2018
University of Liège
2018
Biotechnology Research Institute
2017
Eötvös Loránd University
1997
Carlsberg Laboratory
1995
RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena interference animals posttranscriptional gene (PTGS) plants. In plants, PTGS acts as an antiviral system; successful virus infection requires suppression or evasion induced response. Small interfering RNAs (siRNAs) accumulate plants infected with positive-strand viruses provide specificity to this RNA-mediated defense. We present here results survey virus-specific siRNAs characterized by sequence analysis...
Recent developments in high-throughput sequencing (HTS), also called next-generation (NGS), technologies and bioinformatics have drastically changed research on viral pathogens spurred growing interest the field of virus diagnostics. However, reliability HTS-based detection protocols must be evaluated before adopting them for Many different algorithms aimed at detecting viruses HTS data been reported but little attention has paid thus far to their sensitivity diagnostic purposes. Therefore,...
Short regulatory RNA-s have been identified as key regulators of gene expression in eukaryotes. They involved the regulation both physiological and pathological processes such embryonal development, immunoregulation cancer. One their relevant characteristics is high stability, which makes them excellent candidates for use biomarkers. Their number constantly increasing next generation sequencing methods reveal more details synthesis. These novel findings aim new detection individual short...
Infection of Wheat dwarf virus (WDV) strains on barley results in disease, imposing severe economic losses crop production. As the natural resistance resources against this are limited, it is imperative to elaborate a biotechnological approach that will provide effective and safe immunity wide range WDV strains. Because vector insect-mediated infection occurs during cool periods nature, important identify technology which at lower temperature. In study, we designed artificial microRNAs...
MicroRNAs (miRNAs) are an abundant class of small, endogenous non-protein-coding RNAs, approximately 21 nucleotides in length, that modulate the expression animal and plant target genes at post-transcriptional level. Recent work has shown miRNA-based gene regulation plays a crucial role pathways involved growth development. However, knowledge about timing spatial miRNA is still limited. Here we used situ analysis to demonstrate miRNAs accumulate spatially temporally highly restricted manner...
Summary Various plant viruses ubiquitously mediate the induction of mi R 168, resulting in control ARGONAUTE 1 ( AGO1 ), which is pivotal component microRNA miRNA ) regulation pathway and can also exhibit antiviral function. Here, we demonstrate that 168‐driven persist for a long time virus‐infected plants be an important symptom development. We show infection RNA belonging to various genera associated with transcriptional MIR 168 precursor gene. Moreover, transient expression study, reveal...
As virus diseases cannot be controlled by traditional plant protection methods, the risk of their spread have to minimized on vegetatively propagated plants, such as grapevine. Metagenomic approaches used for diagnostics offer a unique opportunity reveal presence all viral pathogens in investigated plant, which is why application can reduce using infected material new plantation. Here we special branch, deep sequencing virus-derived small RNAs, this high-throughput method diagnostics, and...
Abstract Nowadays, one of the main challenges is moving towards an eco-sustainable agriculture, able to preserve food production through a reduced use pesticides. Current global sustenance by intensive agriculture mainly based on economic crop monocultures and drastically reduces biodiversity, increasing yield losses due presence biotic abiotic stresses. A technology plasma activated water (PAW), characterized in liquid reactive oxygen nitrogen species, was tested try ensure stability also...
Understanding of virus infection-induced alterations in host plant gene expression and metabolism leading to the development disease symptoms is both scientifically economically important. Here, we show that viruses belonging various RNA families are able induce efficient mRNA downregulation (shut-off) systemically infected leaves. We demonstrate shut-off overlaps spatially with virus-occupied sectors, indicating direct role accumulation this phenomenon. The establishment was not directly...
Nicotiana benthamiana is a widely used model plant species for research on plant-pathogen interactions as well other areas of science. It can be easily transformed or agroinfiltrated, therefore it commonly in studies requiring protein localization, interaction, plant-based systems expression and purification. To discover characterize the miRNAs their cleaved target mRNAs N. benthamiana, we sequenced small RNA transcriptomes degradomes two accessions validated them by Northern blots.We...
IL-4-driven alternative macrophage activation and proliferation are characteristic features of both antihelminthic immune responses wound healing in contrast to classical activation, which primarily occurs during inflammatory responses. The signaling pathways defining the genome-wide microRNA expression profile as well cellular functions controlled by microRNAs largely unknown. Hence, current work we examined regulation function IL-4-regulated human mouse activation. We utilized...
High-throughput sequencing (HTS) was employed for the first time to investigate plant viruses infecting solanaceous crops, including potato (Solanum tuberosum), tomato lycopersicum), and pepper (Capsicum annuum), in Kosovo. Leaf samples showing virus-like symptoms were collected from various regions during summer of 2023. Based on ribodepleted RNA bioinformatics analysis, six identified: cucumber mosaic virus, broad bean wilt virus 2 (BBWV2), Y, cryptic (PCV2), bell endornavirus (BPEV),...
Plant viruses ubiquitously mediate the induction of miR168 trough activities viral suppressors RNA silencing (VSRs) controlling accumulation ARGONAUTE1 (AGO1), one main components based host defence system. Here we used a mutant Tombusvirus p19 VSR (p19-3M) disabled in its suppressor function, small interfering (siRNA) binding, to investigate biological role VSR-mediated induction. Infection with virus carrying p19-3M resulted suppressed recovery phenotype despite presence free specific...
Viruses have different strategies for infecting their hosts. Fast and acute infections result in the development of severe symptoms may cause death plant. By contrast, a persistent interaction, virus can survive within its host long time, inducing only mild symptoms. In this study, we investigated gene expression changes induced CymRSV-, crTMV-, TCV-infected Nicotiana benthamiana PVX- TMV-U1-infected Solanum lycopersicum plants after systemic spread by two high-throughput methods: microarray...
Abstract Several viruses are known to infect stone fruit trees and cause serious problems in their cultivation; hence, it is essential use virus-free healthy propagation material establish a plantation. As propagated vegetatively, both the scion rootstock should be pathogen-free. The traditional method for plant virus eradication meristem culture after vivo thermotherapy. Prunus species extremely sensitive high temperature have low regeneration capacity from explants which makes difficult...
The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report molecular analysis computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that mir-282 gene is source 4.9-kb-long primary transcript with 5' cap and 3'-poly(A) sequence mature ∼25 bp. Our data strongly suggest existence an independent conserved holometabolic insects. give evidence locus encodes functional...
Abstract Micro RNAs (miRNAs) are processed from precursor RNA molecules with precisely defined secondary stem-loop structures. ARGONAUTE1 (AGO1) is the main executor component of miRNA pathway and its expression controlled via auto-regulatory feedback loop activity miR168 in plants. Previously we have shown that AGO1 loading strongly restricted leading to abundant cytoplasmic accumulation AGO-unbound miR168. Here, report, intrinsic structure MIR168a not only defines processing miR168, but...
Grapevine Pinot gris virus (GPGV) was described in Italy using a metagenomic approach: next-generation sequencing of the virus-derived small RNAs. Since that time, it has been reported all over world. The presence GPGV is associated with grapevine disease, but most disease asymptomatic. Although host range this not investigated, found non-Vitis hosts, Silene latifolia and Chenopodium album. We investigated other plant species growing as weeds vineyard. Using RT-PCR, we identified seven...