A5072258045- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Physiological and biochemical adaptations
- Insect Resistance and Genetics
- Animal Behavior and Reproduction
- Skin and Cellular Biology Research
- RNA and protein synthesis mechanisms
- Hippo pathway signaling and YAP/TAZ
- Hungarian Social, Economic and Educational Studies
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Mosquito-borne diseases and control
- Microtubule and mitosis dynamics
- ATP Synthase and ATPases Research
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- Silkworms and Sericulture Research
- Animal Genetics and Reproduction
- Studies on Chitinases and Chitosanases
HUN-REN Szegedi Biológiai Kutatóközpont
2020-2025
Dynamique du noyau
2025
Institute of Genetics
2007-2022
Institute of Biochemistry
2020-2021
Hungarian Academy of Sciences
2007-2015
University of Szeged
2014
Abstract During early embryogenesis the zygotic genome remains transcriptionally silent and expression relies on maternally deposited products. Maternal deposition of histones is crucial to preserve chromatin integrity during embryo development, when number nuclei exponentially increases in absence expression. In Drosophila embryo, are as both proteins mRNAs. Histone transcripts only nonpolyadenylated cellular They contain a highly conserved 3′UTR stem-loop structure, which recognized by...
The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report molecular analysis computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that mir-282 gene is source 4.9-kb-long primary transcript with 5' cap and 3'-poly(A) sequence mature ∼25 bp. Our data strongly suggest existence an independent conserved holometabolic insects. give evidence locus encodes functional...
Abstract In most animals, the start of embryogenesis requires specific histones. Drosophila linker histone variant BigH1 is present in early embryos. To uncover role this alternative at embryogenesis, we established fly lines which domains have been replaced partially or completely with that H1. Analysis resulting revealed normal temperature somatic H1 can substitute histone, but low globular and C-terminal are essential for embryogenesis. presence nucleosome stability increases core...
Morphogenesis, the establishment of animal body, requires coordinated rearrangement cells and tissues regulated by a very strictly-determined genetic program. Dorsal closure epithelium in Drosophila melanogaster embryo is one best models for such complex morphogenetic event. To explore regulation dorsal closure, we carried out large-scale RNA interference-based screen combination with vivo time-lapse microscopy identified several genes essential or affecting its dynamics. One novel genes,...
Screening P-element-induced mutant collections, 52 lines were selected as potentially defected ones in endocytosis or autophagy. After excluding those which rescued by 20-hydroxyecdysone treatment, the exact position of inserted P-element was determined remaining lines. In case l(3)S011027 stock, liquid facets (lqf) gene affected codes an epsin-homolog protein Drosophila. We reveal that Lqf is essential to receptor-mediated larval serum proteins (LSPs) fat body cells line, lack fails...
In Drosophila melanogaster, primordial germ cells are specified at the posterior pole of very early embryo. This process is regulated by localized plasm that contains a large number RNAs maternal origin. Transcription in actively down-regulated until cell fate established. Bulk expression zygotic genes commences concomitantly with degradation transcripts. Thus, during embryogenesis, maternally provided and zygotically transcribed mRNAs determine development collectively. an effort to...
Linker histones H1 are essential chromatin components that exist as multiple developmentally regulated variants. In metazoans, specific H1s expressed during germline development in a tightly manner. However, the mechanisms governing their stage-dependent expression poorly understood. Here, we address this question Drosophila , which encodes for single germline-specific dBigH1 linker histone. We show female lineage differentiation, is germ stem cells and cystoblasts, becomes silenced...
dUTP pyrophosphatase (dUTPase) is a dNTP-sanitizing enzyme that prevents the appearance of potentially harmful uracil bases in DNA by hydrolyzing cellular dUTP. This function dUTPase found to be essential many organisms including Drosophila melanogaster. Previously, we showed expression pattern determines extent accumulation genome different tissues. We wished find regulatory mechanism eventually leaves set tissues with uracil-free and intact genome. established promoter overlaps mRNA...
Histone variants are different from their canonical counterparts in structure and encoded by solitary genes with unique regulation to fulfill tissue or differentiation specific functions. A single H4 variant gene (His4r H4r) that is located outside of the histone cluster gives rise a polyA tailed messenger RNA via replication-independent expression preserved Drosophila strains despite its protein product identical H4. In order reveal information on possible role this alternative we epitope...