A5080259965- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Hungarian Social, Economic and Educational Studies
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Classical Studies and Philology
- T-cell and B-cell Immunology
- Prenatal Screening and Diagnostics
- Sulfur Compounds in Biology
- Historical Geopolitical and Social Dynamics
- Electron Spin Resonance Studies
- Ferrocene Chemistry and Applications
- Cultural Identity and Heritage
- Nanoplatforms for cancer theranostics
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Fungal and yeast genetics research
- Advanced Biosensing Techniques and Applications
University of Debrecen
2014-2024
Osiris (Hungary)
2024
Osiris Therapeutics (United States)
2024
Retinoids are morphogens and have been implicated in cell fate commitment of embryonic stem cells (ESCs) to neurons. Their effects mediated by RAR RXR nuclear receptors. However, transcriptional cofactors required for gene-specific retinoid signaling not known. Here we show that protein arginine methyl transferase (PRMT) 1 8 key roles determining regulated gene expression cellular specification a multistage neuronal differentiation model murine ESCs. PRMT1 acts as selective modulator,...
Current approaches have limitations in providing insight into the functional properties of particular nucleosomes their native molecular environment. Here we describe a simple and powerful method involving elution histones using intercalators or salt, to assess stability features dependent on DNA superhelicity relying mainly electrostatic interactions, respectively, measurement fraction remaining chromatin-bound individual nuclei histone type- posttranslational modification- (PTM-) specific...
Bone morphogenetic protein-2 (BMP-2), is a potential factor to enhance osseointegration of dental implants. However, the appropriate cellular system investigate osteogenic effect BMP-2 in vitro standardized manner still needs be defined. The aim this study was examine on cell proliferation and differentiation human progenitors various origins: pulp stem cells (DPSC), osteosarcoma line (Saos-2) embryonic palatal mesenchymal (HEPM). For induction differentiation, culture medium supplemented...
By using a microscopic approach, field inversion single-cell gel electrophoresis, we show that preformed single-strand discontinuities are present in the chromatin of resting and proliferating mammalian yeast cells. These breaks primarily nicks positioned at approximately 50-kbp intervals throughout entire genome could be efficiently labeled situ by DNA polymerase I holoenzyme but not Klenow fragment terminal transferase unless after ribonucleolytic treatments. The RNA molecules involved...
We observed prominent effects of doxorubicin (Dox), an anthracycline widely used in anti-cancer therapy, on the aggregation and intracellular distribution both partners H2A-H2B dimer, with marked differences between two histones. Histone aggregation, assessed by Laser Scanning Cytometry via retention aggregates isolated nuclei, was case H2A. The dominant effect H2B its massive accumulation cytoplasm Jurkat leukemia cells concomitant disappearance from detected confocal microscopy mass...
Abstract In most animals, the start of embryogenesis requires specific histones. Drosophila linker histone variant BigH1 is present in early embryos. To uncover role this alternative at embryogenesis, we established fly lines which domains have been replaced partially or completely with that H1. Analysis resulting revealed normal temperature somatic H1 can substitute histone, but low globular and C-terminal are essential for embryogenesis. presence nucleosome stability increases core...
The restricted access of regulatory factors to their binding sites on DNA wrapped around the nucleosomes is generally interpreted in terms molecular shielding exerted by nucleosomal structure and internucleosomal interactions. Binding proteins often includes intercalation hydrophobic amino acids into DNA. To assess role constrained superhelicity limiting these interactions, we studied small molecule intercalators chromatin close native conditions laser scanning cytometry. We demonstrate that...
In cancer therapy, immunogenic cell death eliminates tumor cells more efficiently than conventional apoptosis. During photodynamic therapy (PDT), some photosensitizer (PS) targeting lysosomes divert apoptosis to the immunologically relevant necrosis-like death. Acridine orange (AO) is a PS lysosome. We synthesized new compound, 3-N,N-dimethylamino-6-isocyanoacridine (DM), modified AO, aiming target better. To compare DM and we studied optical properties, toxicity, internalization,...
Molecular combing and gel electrophoretic studies revealed endogenous nicks with free 3'OH ends at ∼100 kb intervals in the genomic DNA (gDNA) of unperturbed G1-synchronized Saccharomyces cerevisiae cells. Analysis distribution by Nick ChIP-chip indicated that these breaks accumulated active RNA polymerase II (RNAP II) promoters, reminiscent promoter-proximal transient higher eukaryotes. Similar periodicity was found within ribosomal rDNA cluster, involving every ∼10th tandemly repeated 9.1...
Abstract Unexpectedly, the widely used anticancer agents Cisplatin (Cis-Pt) and Daunorubicin (Dauno) exhibited cell type- concentration-dependent synergy or antagonism in vitro . We attempted to interpret these effects terms of changes elicited by drugs chromatin, target held primarily responsible for cytotoxicity both agents. measured effect Cis-Pt on levels Dauno different compartments, Dauno-induced nucleosome eviction, assessed influence DNA platination flow- laser scanning cytometry as...
Abstract Background: Chromatin immunoprecipitation (ChIP) is a widely used technique for the detection of in vivo DNA–protein interactions underlying epigenetic regulation. The standard readout ChIP based on semi‐quantitative or quantitative PCR measurements; however, development alternative platforms with high throughput potentialities expected to facilitate introduction this method into routine diagnostics. Methods: We have established flow‐cytometry‐based evaluation data. capture products...
The roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor eukaryotic gene control, is reported, as measured agarose embedded nuclei H2B-GFP expressor HeLa cells. Nucleosome stability was assessed by quantitative microscopy [[1], [2]] situ, close to native state chromatin, preserving constrained topology genomic DNA. A robust destabilizing observed millimolar concentration range case...
Abstract Exploring the possibilities offered by flow cytometric microbead analyses for detection of genetic alterations, an assay based on dependence melting point double‐stranded DNA molecules their length has been developed, making use PCR products carrying biotin and fluorescent moiety two ends. The samples different immobilized streptavidine coated microbeads are heat‐treated in presence formamide at temperatures between longer that shorter product, when mean fluorescence intensity beads...
Abstract Peptide presentation by MHC class I and II molecules plays important roles in the regulation of immune response. One factor these displays is density antigen, which must exceed a critical threshold for effective activation T cells. Nonrandom distribution has already been detected at nanometer level higher hierarchical levels. It not clear how absence reappearance some protein can influence nonrandom distribution. Therefore, we performed experiments on HLA II‐deficient bare...
We explore the possibilities offered by flow cytometric microbead analysis to develop high throughput methods for detection of deletions/insertions and single-strand DNA lesions. The products PCR reactions derived from reference test samples are denatured reannealed, then exposed enzymatic or chemical treatments distinguishing homoduplices heteroduplices. biotin- dye labeled reaction immobilized on microbeads homo- heteroduplices assessed in separate fluorescence channels, cytometry. Using a...
Epigenetic mechanisms play regulatory roles in dental pulp stem cell (DPSC) differentiation. The molecules that modulate these can be used to enhance DPSC differentiation experimental studies and clinical applications. We investigated the combined effects of an epigenetic modulator enhancer zeste homologue 2 inhibitor (EZH2i), trichostatin A (TSA), 5-azacytidine (5-AZA) on osteogenic DPSCs.
Les deux ouvrages de 1850-51 intitulés Miklós Wesselényi et István Széchenyi sont nés dans l’esprit la polémie tout en interprétant catastrophe 1849, ces brochures influencées par l’ouvrage Plutarque (Vies parallèles des hommes illustres). On remarque confrontation non seulement Wesselényi, mais Széchenyi, plus celle Kossuth avec personnes: Kossuth, dont le comportement a servi d’un modèle possible illustrant pensée politique décennies passées. Ce n’est pas programme qui est critiqué Kemény,...
Fogászati implantáció során a hiányzó fogat vagy fogakat „műgyökerek”, beültetésével pótoljuk. Az integráció folyamatánakfelgyorsítása érdekében az egyik legújabb törekvés bioaktív anyagokkal, növekedési faktorokkal segíteni azimplantátum körül csontképződést. Ilyen molekula amerikai gyógyszer- és élelmiszerügyi hatóság (FDA) által iselfogadott faktor Bone Morfogenic Protein 2 (BMP-2) is. A BMP-2 ilyen jellegű alkalmazását in vitro tesztelésrealkalmas sejtvonalakon vizsgálják. Egyike ezeknek...
Abstract When the effect of various posttranslational histone tail modifications (PTMs) on nucleosome stability was compared in an situ assay involving agarose-embedded nuclei, promoter proximal H3K4me3, H3K27ac and H4K8ac positive nucleosomes exhibited relative sensitivity to intercalators as bulk H3-GFP or carrying any following marks: H3K27me1, H3K27me2, H3K27me3, H3K9me1, H3K9me2, H3K9me3, H3K36me3, H3K4me0, H3K4me1, H3K4me2, H3K9ac, H3K14ac. Nickase DNase I treatment bleomycin live...