A5081353254- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Antifungal resistance and susceptibility
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Fungal Biology and Applications
- Genetics and Neurodevelopmental Disorders
- Animal Behavior and Reproduction
- vaccines and immunoinformatics approaches
- Muscle Physiology and Disorders
- Cancer-related gene regulation
- Plant and Fungal Interactions Research
- Invertebrate Immune Response Mechanisms
- Gut microbiota and health
- Plant Virus Research Studies
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
- Light effects on plants
- Plant Stress Responses and Tolerance
- Insect symbiosis and bacterial influences
University of Szeged
2013-2024
Hungarian Academy of Sciences
2012
Abstract Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but range of suitable host bacterial species limited. This limitation affects both scope identified ARGs and interpretation their clinical relevance. Here we present functional pipeline called Reprogrammed Bacteriophage Particle Assisted Multi-species Metagenomics (DEEPMINE). approach combines improves use T7 bacteriophage with exchanged tail fibres targeted...
Abstract Chronic kidney disease (CKD) is a public health problem that increases the risk of cardiovascular morbidity and mortality. Heart failure with preserved ejection fraction (HFpEF) characterized by left ventricular hypertrophy (LVH) diastolic dysfunction common complication CKD. MicroRNA-212 (miR-212) has been demonstrated previously to be crucial regulator pathologic LVH in pressure-overload-induced heart via regulating forkhead box O3 (FOXO3)/calcineurin/nuclear factor activated...
Posttranslational modification of histones regulates transcription but the exact role that acetylation specific lysine residues plays in biological processes vivo is still not clearly understood. To assess contribution different histone modifications to transcriptional activation vivo, we determined patterns on ecdysone induced Eip74EF and Eip75B genes Drosophila melanogaster larvae by chromatin immunoprecipitation. We found H3 23 localized promoters correlates with endogenous gene...
Huntington's disease (HD) is a dominantly inherited neurodegenerative caused by alterations in the huntingtin gene (htt). Transcriptional dysregulation an early event HD progression. Protein acetylation and methylation particularly on histones regulates chromatin structure thereby preventing or facilitating transcription. Although protein has been found to affect symptoms, little known about potential role of pathology. In recent years, series proteins have described that are responsible for...
Mucormycosis is a life-threatening opportunistic infection caused by certain members of the fungal order Mucorales. This associated with high mortality rate, which can reach nearly 100% depending on underlying condition patient. Treatment mucormycosis challenging because these fungi are intrinsically resistant to most routinely used antifungal agents, such as azoles. One possible mechanism azole resistance drug efflux catalyzed ATP binding cassette (ABC) transporter superfamily. The...
In Drosophila, the dADA2b-containing dSAGA complex is involved in histone H3 lysine 9 and 14 acetylation. Curiously, although 9- 14-acetylated levels are drastically reduced dAda2b mutants, these animals survive until a late developmental stage. To study molecular consequences of loss acetylation, we compared total messenger ribonucleic acid (mRNA) profiles wild type mutant at two stages. Global gene expression profiling indicates that dSAGA-specific acetylation results change (up- or...
Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here transcriptome analysis Drosophila melanogaster dAda2a dAda3 mutants, which subunits ATAC acetyltransferase complex are affected. In agreement with fact these mutations lead to lethality at start metamorphosis, both levels receptor binding polytene chromosomes reduced flies. The cytochrome genes...
Abstract Deficiency of the sleep-wake cycle can accelerate progression Huntington’s disease (HD) and exacerbate symptoms making it a target investigation to better understand molecular pathology disorder. In this study we analyzed sleep defects in Drosophila model HD investigated whether disturbed coincides with alterations mechanism controlling circadian rhythm. To analyze recorded daily activity flies 12:12 hours light:dark entrainment regard underlying measured “clock” gene expression....
Objectives: Green tea infusion contains a complex mixture of polyphenolic compounds that were shown to provide health benefits. It was previously demonstrated (-)-epigallocatechin-3-gallate, one the major polyphenols present in green tea, has suppressing effect on various aspects pathogenesis models Huntington's disease (HD), an inherited neurodegenerative disorder. In this study, we aimed investigate, whether prepared as for human consumption similar positive effects.Methods: We used...
Huntington's disease (HD) is a fatal neurodegenerative disorder caused by the expansion of CAG trinucleotide repeat in Huntingtin gene. Transcriptional dysregulation one main cellular processes affected mutant (mHtt). In this study, we investigate alterations miRNA and mRNA expression levels Drosophila model HD RNA sequencing assess functional effects misregulated miRNAs vivo. We found that head samples flies, level 32 changed significantly; half these were upregulated, while other...
Background Insects have specialized cell types that participate in the elimination of parasites, for instance, lamellocytes broadly studied species Drosophila melanogaster . Other drosophilids, such as ananassae and invasive Zaprionus indianus , multinucleated giant hemocytes, a syncytium blood cells encapsulation eggs or larvae parasitoid wasps. These can be formed by fusion hemocytes circulation originate from lymph gland. Their ultrastructure highly resembles mammalian megakaryocytes....
Ischemic preconditioning (IPre) reduces ischemia/reperfusion (I/R) injury in the heart. The non-coding microRNA miR-125b-1-3p has been demonstrated to play a role mechanism of IPre. Hypercholesterolemia is known attenuate cardioprotective effect preconditioning; nevertheless, exact underlying mechanisms are not clear. Here we investigated, whether hypercholesterolemia influences induction by Male Wistar rats were fed with rodent chow supplemented 2% cholesterol and 0.25% sodium-cholate...
Mucor lusitanicus and some other members of the fungal order Mucorales display phenomenon morphological dimorphism. This means that these fungi aerobically produce filamentous hyphae, developing a coenocytic mycelium, but they grow in multipolar yeast-like form under anaerobiosis. Revealing molecular mechanism reversible yeast-hyphal transition can be interesting for both biotechnological application understanding pathomechanism mucormycosis. In present study, transcriptomic analyses were...
Abstract Post-translational modifications of histone proteins play a pivotal role in DNA packaging and regulation genome functions. Histone acetyltransferase 1 (Hat1) are conserved enzymes that modify histones by acetylating lysine residues. Hat1 is implicated chromatin assembly repair but its cell functions not clearly elucidated. We report the generation characterization loss-of-function mutant Drosophila . mutants viable fertile with mild sub-lethal phenotype showing essential fruit...
ADA2 proteins, together with ADA3, SGF29 and GCN5 form the acetyltransferase module of GNAT-type histone complexes. ADA2b is present in SAGA complex, which plays roles various chromatin-related processes via H3 modifications by other mechanisms.In this report we findings showing that during Drosophila melanogaster development two dADA2b isoforms (dADA2bS dADA2bL) - differ their C-terminal domains are expressed at levels. Genetic complementation experiments indicate dADA2bS alone can support...
A relatively new topic within the field of immunology involves role miRNAs in innate as well adaptive immune response regulation. In recent years, posttranscriptional regulation host-pathogenic fungal interactions through was also suggested.
Assemblysomes are EDTA- and RNase-resistant ribonucleoprotein (RNP) complexes of paused ribosomes with protruding nascent polypeptide chains. They have been described in yeast human cells for the proteasome subunit Rpt1, disordered amino-terminal part chain was found to be indispensable accumulation Rpt1-RNP into assemblysomes. Motivated by this, find other assemblysome-associated RNPs we used bioinformatics rank subunits Saccharomyces cerevisiae protein according their disorder propensity....
Huntington’s disease (HD) is a currently incurable, late onset, progressive, ultimately fatal neurological disorder (Bates et al., 2015). We have recently published the results of comprehensive genetic interaction tests aimed at identification histone methyltransferases and demethylases involved in HD pathogenesis Drosophila model (Song 2018). The methylation state proteins regulates accessibility chromatin structure by which it may influence transcriptional dysregulation observed HD. found...
Abstract Plant infections caused by fungi lead to significant crop losses worldwide every year. This study aims better understand the plant defence mechanisms regulated red light, in particular, effects of light at night when most phytopathogens are highly infectious. Our results showed that superoxide production significantly increased immediately after exposure and, together with hydrogen peroxide levels, was highest dawn 30 min nocturnal red‐light treatment. In parallel, red‐light‐induced...
Huntington’s Disease (HD) is a fatal neurodegenerative disorder caused by the expansion of polyglutamine-coding CAG repeat in Huntingtin gene. One main causes neurodegeneration HD transcriptional dysregulation that, part, inhibition histone acetyltransferase (HAT) enzymes. pathology can be alleviated increasing activity specific HATs or inhibiting deacetylase (HDAC) To determine which histone’s post-translational modifications (PTMs) might play crucial roles pathology, we investigated...
Abstract Huntington’s disease (HD) is a neurodegenerative disorder caused by dominant gain-of-function mutation in the huntingtin gene, resulting an elongated polyglutamine repeat mutant Huntingtin (mHtt) that mediates aberrant protein interactions. Previous studies implicated ubiquitin–proteasome system HD, suggesting restoring cellular proteostasis might be key element suppressing pathology. We applied genetic interaction tests Drosophila model to ask whether modulating levels of...
We previously screened the total nucleic acid extracts of 123 Mucor strains for presence dsRNA molecules without further molecular analyses. Here, we characterized five novel genomes isolated from four different Mucor hiemalis with next-generation sequencing (NGS), namely hiemalis virus 1a (MhV1a) WRL CN(M) 122; 1b (MhV1b) NRRL 3624; 2 (MhV2) 3616; and 3 (MhV3) (MhV4) 3617 strains. Genomes contain two open reading frames (ORF), which encode coat protein (CP) RNA dependent polymerase...