A5061832826- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Peptidase Inhibition and Analysis
- Mycotoxins in Agriculture and Food
- Nail Diseases and Treatments
- Fungal Biology and Applications
- Blood disorders and treatments
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Immune Response and Inflammation
- Pneumocystis jirovecii pneumonia detection and treatment
- Mycorrhizal Fungi and Plant Interactions
- Fermentation and Sensory Analysis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Microbial Natural Products and Biosynthesis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Infectious Diseases and Mycology
- Microbial Metabolic Engineering and Bioproduction
- Oral Health Pathology and Treatment
- Phytoplasmas and Hemiptera pathogens
University of Szeged
2016-2025
Hungarian Academy of Sciences
2021
Albert Einstein College of Medicine
2007-2009
Office of Infectious Diseases
2009
Yeshiva University
2007-2008
Immunovaccine (Canada)
2007
Universität Hamburg
2003-2007
Candida parapsilosis is a major cause of human disease, yet little known about the pathogen's virulence. We have developed an efficient gene deletion system for C. based on repeated use dominant nourseothricin resistance marker (caSAT1) and its subsequent by FLP-mediated, site-specific recombination. Using this technique, we deleted lipase locus in genome consisting adjacent genes CpLIP1 CpLIP2. Additionally reconstructed CpLIP2 gene, which restored activity. Lipolytic activity was absent...
Methamphetamine (Meth) is abused by over 35 million people worldwide. Chronic Meth abuse may be particularly devastating in individuals who engage unprotected sex with multiple partners because it associated a 2-fold higher risk for obtaining HIV and secondary infections. We report the first specific evidence that at pharmacological concentrations exerts direct immunosuppressive effect on dendritic cells macrophages. As weak base, collapses pH gradient across acidic organelles, including...
Candida parapsilosis and albicans are human fungal pathogens that belong to the CTG clade in Saccharomycotina. In contrast C. albicans, relatively little is known about virulence properties of parapsilosis, a pathogen particularly associated with infections premature neonates. We describe here construction strains carrying double allele deletions 100 transcription factors, protein kinases species-specific genes. Two independent were constructed for each target gene. Growth >40 conditions was...
Candida metapsilosis is a rarely-isolated, opportunistic pathogen that belongs to clade of pathogenic yeasts known as the C. parapsilosis sensu lato species complex. To gain insight into recent evolution and genetic basis its virulence, we sequenced genome 11 clinical isolates from various locations, which compared each other available genomes two remaining members complex: orthopsilosis parapsilosis. Unexpectedly, found compelling genomic evidence highly heterozygous hybrid species, with...
The Candida parapsilosis species complex comprises a group of emerging human pathogens varying virulence. This was recently subdivided into three different species: C. sensu stricto, metapsilosis, and orthopsilosis. Within the latter, at least two clearly distinct subspecies seem to be present among clinical isolates (Type 1 Type 2). To gain insight genomic differences between these subspecies, we undertook sequencing isolate classified as compared it with available sequence 2 strain....
Candida auris was first described in Japan 2009 and has now been the cause of significant outbreaks across globe. The high number isolates that are resistant to one or more antifungals, as well mortality rates from patients with bloodstream infections, attracted attention medical mycology, infectious disease, public health communities this pathogenic fungus. In current work, we performed a broad multi-omics approach on two clinical isolated New York, most affected area United States found...
Oral squamous cell carcinoma (OSCC) is a serious health issue worldwide that accounts for 2% to 4% of all cancer cases. Previous studies have revealed higher yeast carriage and diversity in oral patients than healthy individuals.
The PD-1 costimulatory receptor inhibits T cell signaling upon interacting with its ligands PD-L1 and PD-L2. PD-1/PD-L pathway is critical in maintaining self-tolerance. In this study, we examined the role of a mouse model acute infection Histoplasma capsulatum, major human pathogenic fungus. lethal histoplasmosis, all PD-1-deficient mice survived infection, whereas wild-type died disseminated disease. PD-L expression on macrophages splenocytes was up-regulated during from infected inhibited...
The C. parapsilosis sensu lato group involves three closely related species, stricto, orthopsilosis and metapsilosis. Although their overall clinical importance is dramatically increasing, there are few studies regarding the virulence properties of species psilosis complex. In this study, we tested 63 12 metapsilosis 18 isolates for ability to produce extracellular proteases, secrete lipases form pseudohyphae. Significant differences were noted between with strains failing lipase or Nine...
Candida parapsilosis is an important, emerging opportunistic fungal pathogen. Highly mannosylated cell wall proteins are initial contact points with host immune systems. In albicans, Och1 a Golgi 1,6-mannosyltransferase that plays key role in the elaboration of N-linked mannan outer chain. Here, we disrupted C. OCH1 to gain insights into contribution mannosylation fitness and interactions cells. Loss resulted cellular aggregation, failure morphogenesis, enhanced susceptibility perturbing...
The systemic infections caused by members of the Candida parapsilosis complex are currently associated to high mobility and mortality rates, considered as relevant those albicans. Since fungal cell wall is first point contact with host cells, here we performed a comparison this organelle in C. complex, its relevance during interaction human peripheral blood mononuclear cells. We found that similar composition, but differs from albicans, less mannan content more β-glucan porosity levels....
Vulvovaginal candidiasis (VVC) is a common clinical condition with symptoms and signs of vaginal inflammation in the presence Candida species. At least one episode VVC experienced up to 75% women reproductive age group during their lifetime, 5% 8% such suffer from chronic form. Most cases are still caused by C. albicans. However, incidence non-albicans (NAC) species, as parapsilosis, continuously increasing. Despite prevalence NAC, little known about these species almost nothing mechanisms...
In recent years, the relevance of diseases associated with fungal pathogens increased worldwide. Members Candida genus are responsible for greatest number bloodstream infections every year. Epidemiological data consistently indicate a modest shift toward non-albicans species, albeit Candidaalbicans is still most recognizable species within genus. As result, clinically relevant has increased, and, despite their distinct pathogenicity features, applicable antifungal agents remained same. For...
Secreted lipases of Candida albicans are encoded by a gene family with at least 10 members (LIP1-LIP10). The expression pattern this multigene was investigated using reverse transcription polymerase chain reaction in experimental infections and samples patients suffering from oral candidosis. findings illustrate that individual lipase genes differentially regulated mouse model systemic candidosis some showing sustained others being transiently expressed or even silent. profile depended on...
The production of lipases can affect microbial fitness and virulence. We examined the role lipase 8 (LIP8) gene in virulence Candida albicans by constructing Deltalip8 strains URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated absence LIP8 expression homozygous knockout mutants. Reconstituted overexpression mutants were generated introducing a open reading frame under control constitutive actin promoter. Knockout produced more mycelium, particularly at higher...
The effect of methamphetamine on the host response to an opportunistic pathogen has not been extensively described. Methamphetamine is a major public health and safety problem in United States. Chronic abuse associated with 2-fold higher risk human immunodeficiency virus infection and, possibly, additional infections. Histoplasma capsulatum dimorphic fungus that endemic Midwest States causes respiratory systemic disease, particularly individuals impaired immunity. We showed abrogates normal...
Invasive candidiasis is the most commonly reported invasive fungal infection worldwide. Although Candida albicans remains main cause, incidence of emerging species, such as C. parapsilosis increasing. It has been postulated that clinical isolates result from a recent global expansion virulent clone. However, availability single genome for this species so far prevented testing hypothesis at genomic scales. We present here sequence three additional strains and environmental samples. Our...
Multidrug resistance is a common reason behind the failure of chemotherapy. Even if therapy effective, serious adverse effects might develop due to low specificity and selectivity antineoplastic agents. Mesoporous silica nanoparticles (MSNs) are promising materials for tumor-targeting drug-delivery their small size, relatively inert nature, extremely large specific surfaces that can be functionalized by therapeutic targeting entities. We aimed create fluorescently labeled MSN-based system...
Abstract Background Hybrids are chimeric organisms with highly plastic heterozygous genomes that may confer unique traits enabling the adaptation to new environments. However, most evolutionary theory frameworks predict high levels of genetic heterozygosity present in hybrids from divergent parents likely result numerous deleterious epistatic interactions. Under this scenario, selection is expected favor recombination events resulting loss (LOH) affecting genes involved such negative...