A5022811442- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Plant Disease Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
- Polyamine Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Reproductive tract infections research
- Chromosomal and Genetic Variations
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Oral microbiology and periodontitis research
- Trace Elements in Health
- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Infectious Diseases and Mycology
- RNA and protein synthesis mechanisms
University College Dublin
2016-2025
Conway School of Landscape Design
2019-2022
Universitat Pompeu Fabra
2019
22q11 Ireland
2017
Ball (France)
2014
National Superconducting Cyclotron Laboratory
2014
Michigan State University
2014
National Research Nuclear University MEPhI
2014
Trinity College Dublin
1988-2004
University of Michigan
1991
Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report genome sequences six and compare these related pathogens non-pathogens. There significant expansions cell wall, secreted transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts homozygous three diploid possibly resulting from recent recombination events. Surprisingly, key components mating meiosis pathways missing several...
To date, most fungal phylogenies have been derived from single gene comparisons, or concatenated alignments of a small number genes. The increase in genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. As tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees infer relationships between 42 species fungi which complete sequences are available. A dataset 345,829 genes was extracted publicly available Supertree...
Malassezia is a unique lipophilic genus in class Malasseziomycetes Ustilaginomycotina, (Basidiomycota, fungi) that otherwise consists almost exclusively of plant pathogens. are typically isolated from warm-blooded animals, dominant members the human skin mycobiome and associated with common disorders. To characterize genetic basis phenotypes spp., we sequenced genomes all 14 accepted species used comparative genomics against broad panel fungal to comprehensively identify distinct features...
Two independent pathways of transcriptional regulation that show functional homology have been identified in yeast. It has demonstrated previously SWI5 encodes a zinc finger DNA-binding protein whose transcription and cellular localization both are cell cycle regulated. We ACE2, region is nearly identical to SWI5, shows patterns cycle-regulated nuclear similar those seen for SWI5. Despite their similarities, ACE2 function separate regulation. activator the HO endonuclease gene, whereas not....
The genetics of the mating-type ( MAT ) locus have been studied extensively in Saccharomyces cerevisiae , but relatively little is known about how this complex system evolved. We compared organization and mating-type-like MTL loci nine species spanning hemiascomycete phylogenetic tree. inferred that evolved a two-step process which silent HMR / HML cassettes appeared, followed by acquisition Ho endonuclease from mobile genetic element. Ho-mediated switching between an active exists only...
Candida dubliniensis is the closest known relative of albicans , most pathogenic yeast species in humans. However, despite both sharing many phenotypic characteristics, including ability to form true hyphae, C. a significantly less virulent and versatile pathogen. Therefore, identify -specific genes that may be responsible for an increased capacity cause disease, we have sequenced genome compared it with sequence. Although two sequences are highly similar synteny conserved throughout, 168...
Candida parapsilosis and albicans are human fungal pathogens that belong to the CTG clade in Saccharomycotina. In contrast C. albicans, relatively little is known about virulence properties of parapsilosis, a pathogen particularly associated with infections premature neonates. We describe here construction strains carrying double allele deletions 100 transcription factors, protein kinases species-specific genes. Two independent were constructed for each target gene. Growth >40 conditions was...
ABSTRACT The regulation of the response Candida albicans to hypoxic (low-oxygen) conditions is poorly understood. We used microarray and other transcriptional analyses investigate role Upc2 Bcr1 transcription factors in controlling expression genes involved cell wall metabolism, ergosterol synthesis, glycolysis during adaptation hypoxia. Hypoxic induction pathway mimicked by treatment with sterol-lowering drugs (ketoconazole) requires UPC2 . Expression three members family CFEM ( c ommon...
Malassezia commensal yeasts are associated with a number of skin disorders, such as atopic eczema/dermatitis and dandruff, they also can cause systemic infections. Here we describe the 7.67-Mbp genome sympodialis, species eczema, contrast its repertoire that globosa, well those other closely related fungi. Ninety percent predicted M. sympodialis protein coding genes were experimentally verified by mass spectrometry at level. We identified relatively limited to lipid biosynthesis, both lack...
The Candida Gene Order Browser (CGOB) was developed as a tool to visualize and analyze synteny relationships in multiple species, provide an accurate, manually curated set of orthologous genes for evolutionary analyses. Here, we describe major improvements CGOB. underlying structure the database has been changed significantly. Genomic features are now based directly on genome annotations rather than protein sequences, which allows non-protein such centromere locations albicans tRNA all...
Metagenomics uses nucleic acid sequencing to characterize species diversity in different niches such as environmental biomes or the human microbiome. Most studies have used 16S rRNA amplicon identify bacteria. However, decreasing cost of has resulted a gradual shift away from analyses and towards shotgun metagenomic sequencing. Shotgun data can be wide range species, but rarely been applied fungal identification. Here, we develop sequence classification pipeline, FindFungi, use it sequences...
Summary Morphogenesis between yeast and hyphal growth is a characteristic associated with virulence in Candida albicans involves changes the cell wall. In Saccharomyces cerevisiae , transcription factor pair Ace2p Swi5p are key regulators of wall metabolism. Here, we have characterized CaACE2 gene, which encodes only C. homologue S. ACE2 SWI5 . Deleting results defect separation, increased invasion solid agar medium inappropriate pseudohyphal growth, even absence external inducers. The...
The wealth of comparative genomics data from yeast species allows the molecular evolution these eukaryotes to be studied in great detail. We used “proximity plots” visually compare chromosomal gene order information 14 hemiascomycetes, including recent Génolevures survey, Saccharomyces cerevisiae . Contrary original reports, we find that strongly support hypothesis S. is a degenerate polyploid. Using alone, 70% genome can mapped into “sister” regions tile together with almost no overlap....
The yeast transcription factors Ace2p and Swi5p regulate the expression of several target genes involved in mating type switching, exit from mitosis cell wall function. We describe analysis 12 novel targets, some regulated by or alone both. show that is major regulator four ( CTS1 , YHR143W SCW11 YER124C ). Expression all inhibited Swi5p. Like Cts1p Scw11p, two new targets are associated with metabolism. Yhr143p localized to wall, deletion affects separation enhances pseudohyphal growth....
ABSTRACT Ace2 transcription factor family genes are found in many fungal genomes and required for regulation of expression involved cell separation. We used transcriptional profiling to identify the targets Candida albicans , we show that these include several wall components, such as glucanases glycosylphosphatidylinositol-anchored proteins. Expression is downregulated ace2 deletion mutants both yeast hyphal cells. In addition, deleting results dramatic changes metabolic pathways....
Generation of biofilms by the pathogenic yeast Candida parapsilosis is correlated closely with disease. The phenomenon phenotype switching in 20 isolates C. was examined and relationship biofilm development investigated. Four stable heritable phenotypes were identified – crepe, concentric, smooth crater. Cells from crepe concentric are almost entirely pseudohyphal, whilst cells crater mostly yeast-like. pseudohyphae approximately 45 % wider than those cells. cell size smaller other three...
We determined the genome-wide environmental stress response (ESR) expression profile of Candida glabrata, a human pathogen related to Saccharomyces cerevisiae. Despite different habitats, C. S. cerevisiae, Schizosaccharomyces pombe and albicans have qualitatively similar ESR. investigate function glabrata syntenic orthologues ESR transcription factor Msn2. The CgMsn2 CgMsn4 contain motif previously referred as HD1 (homology domain 1) also present in Msn2 from fungi closely show that regions...