A5010595989- Fungal and yeast genetics research
- Antifungal resistance and susceptibility
- Microbial Metabolic Engineering and Bioproduction
- Fungal Infections and Studies
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Polysaccharides and Plant Cell Walls
- Slime Mold and Myxomycetes Research
- Probiotics and Fermented Foods
- Protist diversity and phylogeny
- Diatoms and Algae Research
- Plant Reproductive Biology
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- DNA Repair Mechanisms
- Transgenic Plants and Applications
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Chemical synthesis and alkaloids
University of Sheffield
2008-2019
UiT The Arctic University of Norway
1978
University of Leicester
1973-1976
Candida species are the most common cause of opportunistic fungal infection worldwide. Here we report genome sequences six and compare these related pathogens non-pathogens. There significant expansions cell wall, secreted transporter gene families in pathogenic species, suggesting adaptations associated with virulence. Large genomic tracts homozygous three diploid possibly resulting from recent recombination events. Surprisingly, key components mating meiosis pathways missing several...
A central technique used to investigate the role of a Candida albicans gene is study phenotype cell in which both copies have been deleted. To date, such investigations can only be undertaken if not essential. We describe use MET3 promoter express conditionally an essential gene, so that consequences depletion product may investigated. The effects environmental conditions on its expression were investigated, using GFP as reporter gene. showed ≈85‐fold range expression, according presence or...
Fungi grow with a variety of morphologies: oval yeast cells, chains elongated cells called pseudohyphae and long, narrow, tube-like filaments hyphae. In filamentous fungi, hyphal growth is strongly polarised to the tip mediated by Spitzenkörper, which acts as supply centre concentrate delivery secretory vesicles tip. budding Saccharomyces cerevisiae, polarisome, surface cap proteins that nucleates formation actin cables delivering growing The human fungal pathogen, Candida albicans, can in...
The location of the septin ring in germ tubes Candida albicans hyphae and pseudohyphae was studied using an antibody to Saccharomyces cerevisiae Cdc11p. In induced by growth at 35°C YEPD or Lee's medium, a formed near (mean 1.8 µm) neck between mother cell tube. This became double later cycle, first mitosis took place across plane this ring. A also tube developing serum on medium 37°C. However, times, disorganized disappeared. second then appeared 10–15 µm 12.5 along length nucleus...
Abstract Techniques are described for the induction, isolation, and characterization of mutants Hansenula polymorpha . In addition, techniques controlled passage through life cycle genetic analyses, including complementation, tetrad random spore analysis, have been developed used to assign 62 complementation groups. We report that organism conforms expected genetics a homothallic yeast displays Mendelian segregation genes meiosis. Preliminary mapping data presented indicating linkage three...
During the extreme polarized growth of fungal hyphae, secretory vesicles are thought to accumulate in a subapical region called Spitzenkörper. The human pathogen Candida albicans can grow budding yeast or hyphal form. When it grows as Mlc1 accumulates spot suggestive Spitzenkörper-like structure, while polarisome components Spa2 and Bud6 localize surface crescent. Here we show that vesicle-associated protein Sec4 also localizes spot, confirming putative C. In contrast, exocyst Using...
In the development of hyphal germ tubes Candida albicans, a band septin forms at base tube (basal band). Later, ring forms, which organizes first septum within (septin ring). We have investigated role Nim1 kinases, Gin4 and Hsl1, in formation these structures. show that during formation, is required for organization but not basal band. Hsl1 either rings or bands. Unexpectedly, we found both gin4Δ hsl1Δ mutants form pseudohyphae constitutively, fashion case gin4Δ, partly independent Swe1....
ABSTRACT In Saccharomyces cerevisiae , the G 1 cyclin Cln3 initiates Start of a mitotic cell cycle in response to size and nutrient inputs. Loss delays but does not prevent Start, due eventual Cln3-independent transcription CLN1 CLN2. When unbudded cells human pathogen Candida albicans were depleted they increased did bud. Thus, unlike S. is essential for budding C. albicans. However, eventually large spontaneously produced filamentous forms. The morphology was growth medium dependent; on...
The human fungal pathogen Candida albicans can switch between yeast, pseudohyphal, and hyphal morphologies. To investigate whether the distinctive characteristics of hyphae are due to increased activity Cdc42 GTPase, strains lacking negative regulators were constructed. Unexpectedly, deletion Rho guanine dissociation inhibitor RDI1 resulted in reduced rather than enhanced polarized growth. However, when cells both GTPase-activating proteins, encoded by RGA2 BEM3, grown under...
Summary: A mutant of Hansenula polymorpha defective in β-isopropylmalate dehydrogenase was isolated. It transformed with YEp13, a shuttle vector containing the Saccharomyces cerevisiae LEU2 gene, either using protoplasting method or by treatment LiCl. Leu+ transformants were recovered at frequency 30-60 μg DNA)−1. The shown to contain an autonomously replicating plasmid stability tests, hybridization experiments and recovery YEp13 from cells via transformation Escherichia coli. linearized...
ABSTRACT Candida albicans is a commensal organism that under certain circumstances can become pathogenic. During systemic infection C. disseminated via the circulation to distant organs, where it causes multiple organ failure. Despite severity of infection, little known about mechanisms involved in adhesion this endothelium lining blood vessels. Previous studies have used static assays examine adhesion. However, these do not realistically model vessels, circulating cells must adhere...
ABSTRACT In the hyphal tip of Candida albicans we have made detailed quantitative measurements (i) exocyst components, (ii) Rho1, regulatory subunit (1,3)-β-glucan synthase, (iii) Rom2, specialized guanine-nucleotide exchange factor (GEF) and (iv) actin cortical patches, sites endocytosis. We use resulting data to construct test a 3-dimensional model fungal growth based on proposition that vesicles fuse with at rate determined by local density components. Enzymes such as synthase thus...
The glucose oxidase gene (god) from Aspergillus niger was expressed in Hansenula polymorpha using the methanol promoter and transcription termination region MF-alpha leader sequence Saccharomyces cerevisiae to direct secretion. expression cassette cloned into S. vector YEp13 used transform H. strain A16. In initial transformants plasmid replication unstable, but stabilized by a growth regime consisting of alternating cycles selective non-selective growth. grown high cell density fed-batch...
ABSTRACT The isolation of mutants Saccharomyces cerevisiae that divide at approximately half the size wild type is described. Three have been isolated in which small bud initiation due to a mutation single nuclear gene.