A5042565094- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Cellular transport and secretion
- Ocular Infections and Treatments
- Corneal surgery and disorders
- Bacterial biofilms and quorum sensing
- Polyamine Metabolism and Applications
- Protist diversity and phylogeny
- Wound Healing and Treatments
- Fluorine in Organic Chemistry
- Skin Protection and Aging
- Planarian Biology and Electrostimulation
- Microtubule and mitosis dynamics
- Polysaccharides and Plant Cell Walls
- Ocular Surface and Contact Lens
- Wnt/β-catenin signaling in development and cancer
- Burn Injury Management and Outcomes
- Microbial Metabolites in Food Biotechnology
- Dermatology and Skin Diseases
- Gut microbiota and health
- Chemical Synthesis and Analysis
- melanin and skin pigmentation
- Click Chemistry and Applications
- Biochemical and Structural Characterization
University of Manchester
2016
University of Sheffield
2013-2014
Universidad de Extremadura
2006
Filamentous hyphae of the human pathogen, Candida albicans, invade mucosal layers and medical silicones. In vitro, hyphal tips reorient thigmotropically on contact with small obstacles. It is not known how surface topography sensed but lacking cortical marker, Rsr1/Bud1, are unresponsive. We show that, surfaces, morphology position internal polarity protein complexes asymmetrically skewed towards substratum biased softer two surfaces. nano-fabricated chambers, Spitzenkörper (Spk) responded...
ABSTRACT In the hyphal tip of Candida albicans we have made detailed quantitative measurements (i) exocyst components, (ii) Rho1, regulatory subunit (1,3)-β-glucan synthase, (iii) Rom2, specialized guanine-nucleotide exchange factor (GEF) and (iv) actin cortical patches, sites endocytosis. We use resulting data to construct test a 3-dimensional model fungal growth based on proposition that vesicles fuse with at rate determined by local density components. Enzymes such as synthase thus...
We have characterized the CDC14 gene, which encodes a dual-specificity protein phosphatase in Candida albicans, and demonstrated that its deletion results defects cell separation, mitotic exit morphogenesis. The C. albicans cdc14Δ mutants formed large aggregates of cells resembled those found ace2-null strains. In cells, expression Ace2p target genes was reduced did not accumulate specifically daughter nuclei. Taken together, these imply Cdc14p is required for activation daughter-specific...
In addition to producing melanin protect epidermal keratinocytes against DNA damage, melanocytes may have important roles in strengthening innate immunity pathogens. We developed a functional, pigmented, human full-thickness 3D skin equivalent determine whether the presence of impacts bacterial growth and regulates expression genes involved immune response. introduced primary construct 3-cell with dermal fibroblasts keratinocytes. Immunohistochemistry verified appropriate ratio spatial...
The exocyst, a conserved multiprotein complex, tethers secretory vesicles before fusion with the plasma membrane; thus it is essential for cell surface expansion. In both Saccharomyces cerevisiae and mammalian cells, expansion halted during mitosis. S. cerevisiae, phosphorylation of exocyst component Exo84 by Cdk1-Clb2 mitosis causes to disassemble. Here we show that hyphae human fungal pathogen Candida albicans continue extend throughout whole We CaExo84 phosphorylated Cdk1, which necessary...
Purpose: Some previous reports have established the use of photoactivated chromophore-induced corneal cross-linking (PACK-CXL) in treating fungal keratitis. The results these case often been conflicting. To systematically study effect PACK-CXL management Fusarium keratitis, we developed an ex vivo model human infection using eye-banked corneas. Methods: Sixteen healthy corneas were divided into four groups: (1) untreated control, (2) cross-linked, (3) infected with spores, and (4) spores...
Herein we report the preparation of BODIPY mesoionic acid fluorides through a short sequence involving an isocyanide multicomponent reaction as key synthetic step. These novel are water-stable electrophilic reagents that can be used for fluorescent derivatization amine-containing biomolecules using mild and activation-free conditions. As proof principle, have labeled antifungal natamycin generated fluorogenic probe imaging variety human plant fungal pathogens, with excellent selectivity over...
Candida albicans hyphae grow in a highly polarized fashion from their tips. This growth requires the continuous delivery of secretory vesicles to tip region. Vesicle depends on Sec2p, Guanine Exchange Factor (GEF) for Rab GTPase Sec4p. GTP bound Sec4p is required transit trans-Golgi sites growth. We previously showed that phosphorylation Sec2p at residue S584 was necessary support hyphal, but not yeast Here we show SEC2 mRNA physically associated with Sec2p. Moreover, allows dissociate and...
Burn injuries are a major cause of morbidity and mortality worldwide. Despite advances in therapeutic strategies for the management patients with severe burns, sequelae pathophysiologically profound, up to systemic metabolic levels. Management burn injury is long-term, complex process, treatment dependent on degree location total body surface area (TBSA) affected. In adverse conditions limited resources, efficient triage, stabilisation, rapid transfer specialised intensive care centre...