A5083066953- Antifungal resistance and susceptibility
- Yeasts and Rust Fungi Studies
- Fungal and yeast genetics research
- Oral microbiology and periodontitis research
- Fungal Infections and Studies
- Dental Research and COVID-19
- Salivary Gland Disorders and Functions
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Probiotics and Fermented Foods
- Infectious Diseases and Mycology
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Dental Health and Care Utilization
- Cancer-related molecular mechanisms research
- HIV/AIDS oral health manifestations
- Mycorrhizal Fungi and Plant Interactions
- Insect Resistance and Genetics
- Molecular Biology Techniques and Applications
- Plant-Microbe Interactions and Immunity
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Oral and Craniofacial Lesions
- Coral and Marine Ecosystems Studies
Centre for Genomic Regulation
2015-2025
Universitat Politècnica de Catalunya
2020-2025
Barcelona Supercomputing Center
2020-2025
Institute for Research in Biomedicine
2020-2025
Barcelona Institute for Science and Technology
2017-2023
Universitat Pompeu Fabra
2015-2020
Institute of Science and Technology
2019
The oral cavity comprises a rich and diverse microbiome, which plays important roles in health disease. Previous studies have mostly focused on adult populations or very young children, whereas the adolescent microbiome remains poorly studied. Here, we used citizen science approach 16S profiling to assess of 1500 adolescents around Spain its relationships with lifestyle, diet, hygiene, socioeconomic environmental parameters.Our results provide detailed snapshot how it varies lifestyle other...
Candida metapsilosis is a rarely-isolated, opportunistic pathogen that belongs to clade of pathogenic yeasts known as the C. parapsilosis sensu lato species complex. To gain insight into recent evolution and genetic basis its virulence, we sequenced genome 11 clinical isolates from various locations, which compared each other available genomes two remaining members complex: orthopsilosis parapsilosis. Unexpectedly, found compelling genomic evidence highly heterozygous hybrid species, with...
Candida glabrata is an opportunistic fungal pathogen that ranks as the second most common cause of systemic candidiasis. Despite its genus name, this yeast more closely related to model Saccharomyces cerevisiae than other pathogens, and hence ability infect humans thought have emerged independently. Moreover, C. has all necessary genes undergo a sexual cycle but considered asexual organism due lack direct evidence reproduction. To reconstruct recent evolution find footprints reproduction, we...
Fungal infections are a growing medical concern, in part due to increased resistance one or multiple antifungal drugs. However, the evolutionary processes underpinning acquisition of drug poorly understood. Here, we used experimental microevolution study adaptation yeast pathogen Candida glabrata fluconazole and anidulafungin, two widely drugs with different modes action. Our results show widespread ability rapid both Resistance, including multidrug resistance, is often acquired at moderate...
The relevance of the human oral microbiome to our understanding health has grown in recent years as studies continue develop. Given links cavity with digestive, respiratory and circulatory systems, composition is relevant beyond just health, impacting systemic processes across body. However, we still have a very limited about intrinsic extrinsic factors that shape healthy microbiome. Here, followed citizen-science approach assess relative impact on selected biological, social, lifestyle 1648...
Hybridisation is a common event in yeasts often leading to genomic variability and adaptation. The yeast Candida orthopsilosis human-associated opportunistic pathogen belonging the parapsilosis species complex. Most C. clinical isolates are hybrids resulting from at least four independent crosses between two parental lineages, of which only one has been identified. rare presence or total absence parentals amongst hypothesised be consequence reduced pathogenicity with respect their hybrids....
Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although mechanisms underlying virulence and drug resistance in C. are now starting to be elucidated, we still lack a good understanding how this yeast adapts during course infection. Outstanding questions whether observed genomic plasticity plays role infection, or what levels genetic variation exist within infecting clonal population. To shed light onto populations,...
Abstract Background Hybrids are chimeric organisms with highly plastic heterozygous genomes that may confer unique traits enabling the adaptation to new environments. However, most evolutionary theory frameworks predict high levels of genetic heterozygosity present in hybrids from divergent parents likely result numerous deleterious epistatic interactions. Under this scenario, selection is expected favor recombination events resulting loss (LOH) affecting genes involved such negative...
Candida parapsilosis is an opportunistic fungal pathogen with the ability to cause infections in immunocompromised patients. Echinocandins are currently recommended first line of treatment for all species. Resistance albicans this drug type well characterized. C. strains have lowest vitro susceptibility echinocandins; however, patients such typically respond echinocandin therapy. There little knowledge acquired resistance and its consequences on other characteristics as virulence properties....
Candida glabrata is an opportunistic yeast pathogen thought to have a large genetic and phenotypic diversity highly plastic genome. However, the lack of chromosome-level genome assemblies representing this limits our ability accurately establish how chromosomal structure gene content vary across strains.Here, we expanded publicly available by using long-read sequencing technologies in twelve diverse strains, obtaining final set twenty-one genomes spanning known C. diversity. Using...
Abstract In bilaterian animals, gene regulation is shaped by a combination of linear and spatial regulatory information. Regulatory elements along the genome are integrated into landscapes through chromatin compartmentalization 1,2 , insulation neighbouring genomic regions 3,4 looping that brings together distal cis -regulatory sequences 5 . However, evolution these features unknown because three-dimensional architecture most animal lineages remains unexplored 6,7 To trace evolutionary...
Introduction: The oral cavity harbors an abundant and diverse microbial community (i.e. the microbiome), whose composition roles in health disease have been focus of intense research. Down syndrome (DS) is associated with particular characteristics cavity, a lower incidence caries higher periodontitis gingivitis compared to control populations. However, overall microbiome DS how it varies factors like host age or pH within mouth are still poorly understood. Methods: Using Citizen-Science...
The limited number of available antifungal drugs and the increasing fungal isolates that show drug or multidrug resistance pose a serious medical threat. Several yeast pathogens, such as
The formation of interspecific hybrids results in the coexistence two diverged genomes within same nucleus. It has been hypothesized that negative epistatic interactions and regulatory interferences between sub-genomes may elicit a so-called genomic shock involving, among other alterations, broad transcriptional changes. To assess magnitude this hybrid yeasts, we investigated transcriptomic differences newly formed Saccharomyces cerevisiae × uvarum diploid its parentals, which ∼20 mya. RNA...
Echinocandins are the newest class of antifungal drugs in clinical use. These agents inhibit β-glucan synthase, which catalyzes synthesis β-glucan, an essential component fungal cell wall, and have a high efficacy low toxicity. Echinocandin resistance is largely due to mutations gene encoding but mode action not fully understood. We developed fluorescent probes based on caspofungin, first clinically approved echinocandin, studied their cellular biology Candida species, most common cause...
Each year, infections caused by fungal pathogens claim the lives of about 1.6 million people and affect health over a billion worldwide. Among most recently developed antifungal drugs are echinocandins, which noncompetitively inhibit β-glucan synthase, membrane-bound protein complex that catalyzes formation main polysaccharide component cell wall. Resistance to echinocandins is conferred mutations in FKS genes, encode catalytic subunit synthase complex. Here, we report selective removal...
Long non-coding RNAs (lncRNAs) are a heterogeneous class of genes that do not code for proteins. Since lncRNAs (or fraction thereof) expected to be functional, many efforts have been dedicated catalog in numerous organisms, but our knowledge non vertebrate species remains very limited. Here, we annotated using transcriptomic data from the same larval stage four Caenorhabditis species. The number self-fertile nematodes was lower than out-crossing We used combination approaches identify...
Introduction: Cystic fibrosis (CF) is an autosomal genetic disease, associated with the production of excessively thick mucosa and life-threatening chronic lung infections. The microbiota oral cavity can act as a reservoir or barrier for infectious microorganisms that colonize lungs. However, specific composition microbiome in CF poorly understood.Methods: In collaboration associations Spain, we collected rinse samples from 31 persons (age range 7-47) matched controls, then performed 16S...
Interspecific hybridization can drive evolutionary adaptation to novel environments. The Saccharomycotina clade of budding yeasts includes many hybrid lineages, and has been proposed as a source for new pathogenic species. Candida orthopsilosis is an emerging opportunistic pathogen which most clinical isolates are hybrids, each derived from one at least four independent crosses between the same two parental lineages. To gain insight into transcriptomic aftermath in these pathogens, we...
Abstract Candida glabrata is an opportunistic fungal pathogen that ranks as the second most common cause of systemic candidiasis. Despite its genus name, this yeast more closely related to model Saccharomyces cerevisiae than other pathogens, and hence ability infect humans thought have emerged independently. Morover, C. has all necessary genes undergo a sexual cycle, but it considered asexual organism due lack direct evidence reproduction. Here, we assessed genomic phenotypic variation...
The Nakaseomyces clade is formed by at least nine described species among which three can be pathogenic to humans, namely glabratus (Candida glabrata), the second most-common cause of candidiasis worldwide, and two rarer emerging pathogens: (Candida) nivarensis bracarensis. Early comparative genomics analyses identified parallel expansions subtelomeric adhesin genes in N. nivarensis/bracarensis, suggested possible links with emergence virulence potential these species. However, as shown for...